Restriction map for
pF1KB9737
(Circular) (Six-base) MAPSORT of: pF1KB9737.seq Check: 1885 from: 1 to: 4613
pF1KB9737 4613 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 726 726
Size: 4613
AccI GT'mk_AC
Cuts at: 1651 1651
Size: 4613
Acc65I G'GTAC_C
Cuts at: 1629 1629
Size: 4613
AclI AA'CG_TT
Cuts at: 4453 4453
Size: 4613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1411 2438 2870 3617 3885 634
Size: 777 1027 432 747 268 1362
Fragments arranged by size:
1362 1027 777 747 432 268
AflIII A'CryG_T
Cuts at: 1965 3069 4046 1965
Size: 1104 977 2532
Fragments arranged by size:
2532 1104 977
AgeI A'CCGG_T
Cuts at: 3001 3001
Size: 4613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1300 1300
Size: 4613
AlwNI CAG_nnn'CTG
Cuts at: 1203 1212 1918 3485 1203
Size: 9 706 1567 2331
Fragments arranged by size:
2331 1567 706 9
ApaLI G'TGCA_C
Cuts at: 3383 3383
Size: 4613
ApoI r'AATT_y
Cuts at: 707 1617 3966 4222 707
Size: 910 2349 256 1098
Fragments arranged by size:
2349 1098 910 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4613
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
AvaI C'yCGr_G
Cuts at: 180 1633 180
Size: 1453 3160
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1081 1114 1081
Size: 33 4580
BamHI G'GATC_C
Cuts at: 1638 1638
Size: 4613
BanI G'GyrC_C
Cuts at: 125 256 298 313 1629 2043 2291 2326 125
Size: 131 42 15 1316 414 248 35 2412
Fragments arranged by size:
2412 1316 414 248 131 42 35 15
BanII G_rGCy'C
Cuts at: 136 1067 1265 1627 2657 136
Size: 931 198 362 1030 2092
Fragments arranged by size:
2092 1030 931 362 198
BbeI G_GCGC'C
Cuts at: 302 2047 2295 302
Size: 1745 248 2620
Fragments arranged by size:
2620 1745 248
BbvCI CC'TCA_GC
Cuts at: 196 1013 196
Size: 817 3796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 325 359 325
Size: 34 4579
BciVI GTATCCnnnnn_n'
Cuts at: 1549 2511 3272 4613 1549
Size: 962 761 1341 1549
Fragments arranged by size:
1549 1341 962 761
BclI T'GATC_A
Cuts at: 2133 2133
Size: 4613
BglI GCCn_nnn'nGGC
Cuts at: 215 1321 215
Size: 1106 3507
BglII A'GATC_T
Cuts at: 2128 4165 2128
Size: 2037 2576
BlpI GC'TnA_GC
Cuts at: 764 1243 1725 764
Size: 479 482 3652
Fragments arranged by size:
3652 482 479
Bme1580I G_kGCm'C
Cuts at: 259 1367 1457 1543 2238 2331 3387 259
Size: 1108 90 86 695 93 1056 1485
Fragments arranged by size:
1485 1108 1056 695 93 90 86
BmrI ACTGGGnnnn_n'
Cuts at: 206 1825 1906 2241 4385 206
Size: 1619 81 335 2144 434
Fragments arranged by size:
2144 1619 434 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1056 2980 1056
Size: 1924 2689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 1081 1287 1405 1414 1465 1585 120
Size: 961 206 118 9 51 120 3148
Fragments arranged by size:
3148 961 206 120 118 51 9
Bpu10I CC'TnA_GC
Cuts at: 196 697 1013 1547 196
Size: 501 316 534 3262
Fragments arranged by size:
3262 534 501 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 885 1093 1123 1789 2668 3160 3458 885
Size: 208 30 666 879 492 298 2040
Fragments arranged by size:
2040 879 666 492 298 208 30
BsaI GGTCTCn'nnnn_
Cuts at: 4317 4317
Size: 4613
BsaAI yAC'GTr
Cuts at: 1966 1966
Size: 4613
BsaBI GATnn'nnATC
Cuts at: 6 952 2127 4164 6
Size: 946 1175 2037 455
Fragments arranged by size:
2037 1175 946 455
BsaHI Gr'CG_yC
Cuts at: 227 299 2044 2292 2994 4490 227
Size: 72 1745 248 702 1496 350
Fragments arranged by size:
1745 1496 702 350 248 72
BsaWI w'CCGG_w
Cuts at: 1802 2323 3001 3275 3422 3833 1802
Size: 521 678 274 147 411 2582
Fragments arranged by size:
2582 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 118 148 118
Size: 30 4583
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1048 1138 1273 1048
Size: 90 135 4388
Fragments arranged by size:
4388 135 90
BseYI C'CCAG_C
Cuts at: 306 875 2038 2796 3373 3869 306
Size: 569 1163 758 577 496 1050
Fragments arranged by size:
1163 1050 758 577 569 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 1348 1201
Size: 147 4466
BsiEI CG_ry'CG
Cuts at: 104 1316 2155 2201 3409 104
Size: 1212 839 46 1208 1308
Fragments arranged by size:
1308 1212 1208 839 46
BsiHKAI G_wGCw'C
Cuts at: 1040 1067 1627 2405 2595 3387 1040
Size: 27 560 778 190 792 2266
Fragments arranged by size:
2266 792 778 560 190 27
Bsp1286I G_dGCh'C
Cuts at: 136 259 1040 1067 1265 1367 1457 1543 1627
Size: 123 781 27 198 102 90 86 84
Cuts at: 1627 2238 2331 2405 2595 2657 3387 136
Size: 611 93 74 190 62 730 1362
Fragments arranged by size:
1362 781 730 611 198 190 123 102
93 90 86 84 74 62 27
BspEI T'CCGG_A
Cuts at: 3833 3833
Size: 4613
BspHI T'CATG_A
Cuts at: 587 3789 587
Size: 3202 1411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 726 1085 1220 1579 1663 1877 2179 2560 726
Size: 359 135 359 84 214 302 381 2779
Fragments arranged by size:
2779 381 359 359 302 214 135 84
BsrBI CCG'CTC
Cuts at: 50 980 2905 2959 4443 4608 50
Size: 930 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 930 165 55 54
BsrDI GCAATG_nn'
Cuts at: 431 2525 431
Size: 2094 2519
BsrFI r'CCGG_y
Cuts at: 2611 2792 3001 2611
Size: 181 209 4223
Fragments arranged by size:
4223 209 181
BsrGI T'GTAC_A
Cuts at: 1172 1172
Size: 4613
BssSI C'ACGA_G
Cuts at: 418 1219 2884 3242 418
Size: 801 1665 358 1789
Fragments arranged by size:
1789 1665 801 358
BstAPI GCAn_nnn'nTGC
Cuts at: 320 569 1212 1491 1990 320
Size: 249 643 279 499 2943
Fragments arranged by size:
2943 643 499 279 249
BstBI TT'CG_AA
Cuts at: 2974 2974
Size: 4613
BstXI CCAn_nnnn'nTGG
Cuts at: 273 273
Size: 4613
BstYI r'GATC_y
Cuts at: 1 1182 1638 2105 2128 2463 2709 3721 3807
Size: 1181 456 467 23 335 246 1012 86
Cuts at: 3807 3819 4165 1
Size: 12 346 449
Fragments arranged by size:
1181 1012 467 456 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 806 806
Size: 4613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2563 2744 2907 2563
Size: 181 163 4269
Fragments arranged by size:
4269 181 163
BtsI GCAGTG_nn'
Cuts at: 1225 1291 1541 1856 1225
Size: 66 250 315 3982
Fragments arranged by size:
3982 315 250 66
ClaI AT'CG_AT
Cuts at: 188 188
Size: 4613
DraI TTT'AAA
Cuts at: 1612 1612
Size: 4613
DrdI GACnn_nn'nnGTC
Cuts at: 165 2319 3177 165
Size: 2154 858 1601
Fragments arranged by size:
2154 1601 858
EaeI y'GGCC_r
Cuts at: 368 527 1313 1594 2198 2372 2763 2790 4135
Size: 159 786 281 604 174 391 27 1345
Cuts at: 4135 368
Size: 846
Fragments arranged by size:
1345 846 786 604 391 281 174 159
27
EagI C'GGCC_G
Cuts at: 1313 2198 1313
Size: 885 3728
EarI CTCTTCn'nnn_
Cuts at: 1384 2636 2846 1384
Size: 1252 210 3151
Fragments arranged by size:
3151 1252 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2821 3131 3277 4423 2821
Size: 310 146 1146 3011
Fragments arranged by size:
3011 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1065 1625 1065
Size: 560 4053
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 634 1081 1287 1405 1411 1414 1465 1585
Size: 514 447 206 118 6 3 51 120
Cuts at: 1585 2438 2870 3617 3885 120
Size: 853 432 747 268 848
Fragments arranged by size:
853 848 747 514 447 432 268 206
120 118 51 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1410 1410
Size: 4613
EcoO109I rG'GnC_Cy
Cuts at: 822 1143 1209 1752 822
Size: 321 66 543 3683
Fragments arranged by size:
3683 543 321 66
EcoRI G'AATT_C
Cuts at: 1617 1617
Size: 4613
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 501 2632 501
Size: 2131 2482
FspI TGC'GCA
Cuts at: 2394 2394
Size: 4613
HaeII r_GCGC'y
Cuts at: 302 411 1254 2047 2295 3317 4296 302
Size: 109 843 793 248 1022 979 619
Fragments arranged by size:
1022 979 843 793 619 248 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 453 2455 2485 2487 2517 421
Size: 32 2002 30 2 30 2517
Fragments arranged by size:
2517 2002 32 30 30 2
HincII GTy'rAC
Cuts at: 1652 1652
Size: 4613
KasI G'GCGC_C
Cuts at: 298 2043 2291 298
Size: 1745 248 2620
Fragments arranged by size:
2620 1745 248
KpnI G_GTAC'C
Cuts at: 1633 1633
Size: 4613
MluI A'CGCG_T
Cuts at: 4046 4046
Size: 4613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 3284 3468 248
Size: 3036 184 1393
Fragments arranged by size:
3036 1393 184
MscI TGG'CCA
Cuts at: 1596 2374 1596
Size: 778 3835
MslI CAynn'nnrTG
Cuts at: 2602 2729 2602
Size: 127 4486
MspA1I CmG'CkG
Cuts at: 140 233 350 521 1086 1299 1606 1724 2038
Size: 93 117 171 565 213 307 118 314
Cuts at: 2038 2398 3411 3656 140
Size: 360 1013 245 1097
Fragments arranged by size:
1097 1013 565 360 314 307 245 213
171 118 117 93
NaeI GCC'GGC
Cuts at: 2794 2794
Size: 4613
NarI GG'CG_CC
Cuts at: 299 2044 2292 299
Size: 1745 248 2620
Fragments arranged by size:
2620 1745 248
NgoMIV G'CCGG_C
Cuts at: 2792 2792
Size: 4613
NspI r_CATG'y
Cuts at: 390 1492 1666 2166 3073 390
Size: 1102 174 500 907 1930
Fragments arranged by size:
1930 1102 907 500 174
PasI CC'CwG_GG
Cuts at: 252 912 252
Size: 660 3953
PciI A'CATG_T
Cuts at: 3069 3069
Size: 4613
PflMI CCAn_nnn'nTGG
Cuts at: 1406 1406
Size: 4613
PfoI T'CCnGG_A
Cuts at: 1382 1382
Size: 4613
PmeI GTTT'AAAC
Cuts at: 1612 1612
Size: 4613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4581
PpuMI rG'GwC_Cy
Cuts at: 1209 1209
Size: 4613
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 499 531 499
Size: 32 4581
PstI C_TGCA'G
Cuts at: 938 1085 1208 1232 1355 1660 2345 938
Size: 147 123 24 123 305 685 3206
Fragments arranged by size:
3206 685 305 147 123 123 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4613
PvuII CAG'CTG
Cuts at: 140 521 1086 1606 2038 2398 140
Size: 381 565 520 432 360 2355
Fragments arranged by size:
2355 565 520 432 381 360
RsrII CG'GwC_CG
Cuts at: 2808 2808
Size: 4613
SacI G_AGCT'C
Cuts at: 1067 1627 1067
Size: 560 4053
SalI G'TCGA_C
Cuts at: 1650 1650
Size: 4613
SapI GCTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
SbfI CC_TGCA'GG
Cuts at: 1660 1660
Size: 4613
ScaI AGT'ACT
Cuts at: 4130 4130
Size: 4613
SfcI C'TryA_G
Cuts at: 33 934 1081 1204 1228 1338 1351 1656 2341
Size: 901 147 123 24 110 13 305 685
Cuts at: 2341 3334 3525 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 901 685 305 191 147 123
110 24 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 1321 1321
Size: 4613
SfoI GGC'GCC
Cuts at: 300 2045 2293 300
Size: 1745 248 2620
Fragments arranged by size:
2620 1745 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
SmaI CCC'GGG
Cuts at: 1635 1635
Size: 4613
SmlI C'TyrA_G
Cuts at: 900 1108 1138 1768 2683 3175 3437 900
Size: 208 30 630 915 492 262 2076
Fragments arranged by size:
2076 915 630 492 262 208 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4613
SphI G_CATG'C
Cuts at: 1666 2166 1666
Size: 500 4113
StuI AGG'CCT
Cuts at: 481 577 1405 481
Size: 96 828 3689
Fragments arranged by size:
3689 828 96
StyI C'CwwG_G
Cuts at: 501 1319 1400 1747 2101 501
Size: 818 81 347 354 3013
Fragments arranged by size:
3013 818 354 347 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 300 866 1524 2196 2329 2788 2997 274
Size: 26 566 658 672 133 459 209 1890
Fragments arranged by size:
1890 672 658 566 459 209 133 26
TatI w'GTAC_w
Cuts at: 383 515 1172 4128 383
Size: 132 657 2956 868
Fragments arranged by size:
2956 868 657 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 118 150 4130 4162 118
Size: 32 3980 32 569
Fragments arranged by size:
3980 569 32 32
Tth111I GACn'n_nGTC
Cuts at: 2410 2410
Size: 4613
XbaI T'CTAG_A
Cuts at: 1185 1644 1185
Size: 459 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 211 354 1083 1529 2108 211
Size: 143 729 446 579 2716
Fragments arranged by size:
2716 729 579 446 143
XmaI C'CCGG_G
Cuts at: 1633 1633
Size: 4613
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BbsI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I BtgI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI