Restriction map for pF1KB9716
 (Linear) (Six-base) MAPSORT of: KIBB9716.seq  Check: 9105  from: 1  to: 1179



KIBB9716.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AatII G_ACGT'C

Cuts at:      0     755    1179 
   Size:        755     424
 
AflIII A'CryG_T

Cuts at:      0     881    1179 
   Size:        881     298
 
AleI CACnn'nnGTG

Cuts at:      0      50     137     185    1179 
   Size:         50      87      48     994

  Fragments arranged by size: 

                 994      87      50      48
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     955    1179 
   Size:        955     224
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     784     816    1179 
   Size:        784      32     363

  Fragments arranged by size: 

                 784     363      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     708    1179 
   Size:        708     471
 
AvaI C'yCGr_G

Cuts at:      0      64    1133    1179 
   Size:         64    1069      46

  Fragments arranged by size: 

                1069      64      46
 
BamHI G'GATC_C

Cuts at:      0     544     563    1179 
   Size:        544      19     616

  Fragments arranged by size: 

                 616     544      19
 
BanI G'GyrC_C

Cuts at:      0    1011    1179 
   Size:       1011     168
 
BanII G_rGCy'C

Cuts at:      0     628    1141    1179 
   Size:        628     513      38

  Fragments arranged by size: 

                 628     513      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     747    1179 
   Size:        747     432
 
Bme1580I G_kGCm'C

Cuts at:      0     386     965    1179 
   Size:        386     579     214

  Fragments arranged by size: 

                 579     386     214
 
BmgBI CAC'GTC

Cuts at:      0     102    1179 
   Size:        102    1077
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     532    1047    1179 
   Size:        532     515     132

  Fragments arranged by size: 

                 532     515     132
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     364     953    1179 
   Size:        364     589     226

  Fragments arranged by size: 

                 589     364     226
 
Bpu10I CC'TnA_GC

Cuts at:      0     866    1179 
   Size:        866     313
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     211     335    1179 
   Size:        211     124     844

  Fragments arranged by size: 

                 844     211     124
 
BsaAI yAC'GTr

Cuts at:      0     960    1179 
   Size:        960     219
 
BsaHI Gr'CG_yC

Cuts at:      0     271     752    1179 
   Size:        271     481     427

  Fragments arranged by size: 

                 481     427     271
 
BsaWI w'CCGG_w

Cuts at:      0     413    1179 
   Size:        413     766
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      13      43     784     814     976    1006    1179 
   Size:         13      30     741      30     162      30     173

  Fragments arranged by size: 

                 741     173     162      30      30      30      13
 
BseYI C'CCAG_C

Cuts at:      0     385     457     496     698     807     870    1179 
   Size:        385      72      39     202     109      63     309

  Fragments arranged by size: 

                 385     309     202     109      72      63      39
 
BsiEI CG_ry'CG

Cuts at:      0      87    1179 
   Size:         87    1092
 
BsiHKAI G_wGCw'C

Cuts at:      0     773     934    1179 
   Size:        773     161     245

  Fragments arranged by size: 

                 773     245     161
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     396    1179 
   Size:        396     783
 
Bsp1286I G_dGCh'C

Cuts at:      0     386     628     773     934     965    1141    1179 
   Size:        386     242     145     161      31     176      38

  Fragments arranged by size: 

                 386     242     176     161     145      38      31
 
BspEI T'CCGG_A

Cuts at:      0     413    1179 
   Size:        413     766
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197    1179 
   Size:        197     982
 
BsrFI r'CCGG_y

Cuts at:      0      41      86     220     344    1179 
   Size:         41      45     134     124     835

  Fragments arranged by size: 

                 835     134     124      45      41
 
BstEII G'GTnAC_C

Cuts at:      0     946    1179 
   Size:        946     233
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     282    1179 
   Size:        282     897
 
BstYI r'GATC_y

Cuts at:      0     544     563    1179 
   Size:        544      19     616

  Fragments arranged by size: 

                 616     544      19
 
BtgI C'CryG_G

Cuts at:      0     180    1179 
   Size:        180     999
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      49    1179 
   Size:         49    1130
 
BtsI GCAGTG_nn'

Cuts at:      0       9    1179 
   Size:          9    1170
 
EaeI y'GGCC_r

Cuts at:      0      84     353     480     972    1179 
   Size:         84     269     127     492     207

  Fragments arranged by size: 

                 492     269     207     127      84
 
EagI C'GGCC_G

Cuts at:      0      84    1179 
   Size:         84    1095
 
EarI CTCTTCn'nnn_

Cuts at:      0     399     589    1179 
   Size:        399     190     590

  Fragments arranged by size: 

                 590     399     190
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1131    1179 
   Size:       1131      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     355    1179 
   Size:        355     824
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1127    1179 
   Size:       1127      52
 
FseI GG_CCGG'CC

Cuts at:      0      90    1179 
   Size:         90    1089
 
HaeII r_GCGC'y

Cuts at:      0     527    1179 
   Size:        527     652
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45    1179 
   Size:         13      32    1134

  Fragments arranged by size: 

                1134      32      13
 
HindIII A'AGCT_T

Cuts at:      0     713    1179 
   Size:        713     466
 
MscI TGG'CCA

Cuts at:      0     482     974    1179 
   Size:        482     492     205

  Fragments arranged by size: 

                 492     482     205
 
MslI CAynn'nnrTG

Cuts at:      0      50     137     185    1179 
   Size:         50      87      48     994

  Fragments arranged by size: 

                 994      87      50      48
 
MspA1I CmG'CkG

Cuts at:      0     168     708    1153    1162    1179 
   Size:        168     540     445       9      17

  Fragments arranged by size: 

                 540     445     168      17       9
 
NaeI GCC'GGC

Cuts at:      0      88    1179 
   Size:         88    1091
 
NgoMIV G'CCGG_C

Cuts at:      0      86    1179 
   Size:         86    1093
 
PasI CC'CwG_GG

Cuts at:      0     820    1179 
   Size:        820     359
 
PmlI CAC'GTG

Cuts at:      0     960    1179 
   Size:        960     219
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     784     816    1179 
   Size:        784      32     363

  Fragments arranged by size: 

                 784     363      32
 
PvuII CAG'CTG

Cuts at:      0     168     708    1179 
   Size:        168     540     471

  Fragments arranged by size: 

                 540     471     168
 
ScaI AGT'ACT

Cuts at:      0     799    1179 
   Size:        799     380
 
SmaI CCC'GGG

Cuts at:      0    1135    1179 
   Size:       1135      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     455     712    1179 
   Size:        455     257     467

  Fragments arranged by size: 

                 467     455     257
 
TatI w'GTAC_w

Cuts at:      0     797    1055    1179 
   Size:        797     258     124

  Fragments arranged by size: 

                 797     258     124
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     477     509    1179 
   Size:        477      32     670

  Fragments arranged by size: 

                 670     477      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     261     537     867     996    1179 
   Size:        261     276     330     129     183

  Fragments arranged by size: 

                 330     276     261     183     129
 
XmaI C'CCGG_G

Cuts at:      0    1133    1179 
   Size:       1133      46
 
ZraI GAC'GTC

Cuts at:      0     753    1179 
   Size:        753     426
 

 Enzymes that do cut:

    AatII   AflIII     AleI     AlfI     AloI    AlwNI     AvaI    BamHI
     BanI    BanII     BbsI Bme1580I    BmgBI     BmrI     BplI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspMI    BsrFI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     EaeI     EagI     EarI     EciI    EcoNI
     FalI     FseI    HaeII    Hin4I  HindIII     MscI     MslI   MspA1I
     NaeI   NgoMIV     PasI     PmlI     PpiI    PvuII     ScaI     SmaI
    TaqII     TatI     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmtI     BpmI    BpuEI    BsaBI    BseRI     BsgI
    BsiWI     BsmI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI  Tth111I     XbaI     XhoI     XmnI
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