Restriction map for pF1KB9714
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BcgI        BclI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
BstBI       BstEII      BstPI       Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    RsrII       SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccII          39  CGCG                 CG^CG
AcsI         1128  GAATTC               [GA]^AATT[CT]
AlwI          928  GATCC                GGATC(4/5)
ApaI          326  GGGCCC               GGGCC^C
ApoI         1128  GAATTC               [GA]^AATT[CT]
AspHI         810  GAGCTC               G[AT]GC[AT]^C
AspI          938  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         570  GGACC                G^G[AT]CC
BanI          988  GGTGCC               G^G[CT][GA]CC
BbvCI         556  GCTGAGG              CCTCAGC(-5/-2)
BciVI         646  GTATCC               GTATCC(6/5)
BcnI          225  CCGGG                CC^[GC]GG
BfaI         1057  CTAG                 C^TAG
BglI          532  GCCCATGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI          178  GTGCAG               GTGCAG(16/14)
BsiEI          41  CGATCG               CG[GA][CT]^CG
BsiHKAI       810  GAGCTC               G[AT]GC[AT]^C
BsmI          625  GCATTC               GAATGC(1/-1)
BspHI         857  TCATGA               T^CATGA
BspMI         214  ACCTGC               ACCTGC(4/8)
Bst1107I      340  GTATAC               GTA^TAC
BstAPI        171  GCAGTGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        845  GGATG                GGATG(2/0)
BstUI          39  CGCG                 CG^CG
BstXI         957  CCAGATGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         927  AGATCC               [GA]^GATC[CT]
BstZ17I       340  GTATAC               GTA^TAC
DsaI          534  CCATGG               C^C[GA][CT]GG
EarI          333  GAAGAG               CTCTTC(1/4)
EcoNI         408  CCTGAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1128  GAATTC               G^AATTC
EcoT14I       534  CCATGG               C^C[AT][AT]GG
FokI          845  GGATG                GGATG(9/13)
HaeII         578  AGCGCC               [GA]GCGC^[CT]
HapII         225  CCGG                 C^CGG
HpaII         225  CCGG                 C^CGG
Ksp632I       333  GAAGAG               CTCTTC(1/4)
MaeI         1057  CTAG                 C^TAG
MaeII         588  ACGT                 A^CGT
MflI          927  AGATCC               [GA]^GATC[CT]
MseI          275  TTAA                 T^TAA
MslI          979  CACCCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          225  CCGG                 C^CGG
MvnI           39  CGCG                 CG^CG
NciI          225  CCGGG                CC^[GC]GG
NcoI          534  CCATGG               C^CATGG
NspI          974  ACATGC               [GA]CATG^[CT]
PflFI         938  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1005  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI           41  CGATCG               CGAT^CG
PvuII         421  CAGCTG               CAG^CTG
RcaI          857  TCATGA               T^CATGA
SacI          810  GAGCTC               GAGCT^C
SbfI          659  CCTGCAGG             CCTGCA^GG
SfaNI         960  GATGC                GCATC(5/9)
Sse8387I      659  CCTGCAGG             CCTGCA^GG
StuI          632  AGGCCT               AGG^CCT
StyI          534  CCATGG               C^C[AT][AT]GG
TfiI          854  GAATC                G^A[AT]TC
Tru9I         275  TTAA                 T^TAA
Tsp45I       1046  GTGAC                ^GT[GC]AC
Tsp509I      1129  AATT                 ^AATT
Tth111I       938  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1005  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         927  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI          340  GTATAC               GT^[AC][GT]AC
              916  GTCTAC              
AfaI          240  GTAC                 GT^AC
             1116  GTAC                
BalI          758  TGGCCA               TGG^CCA
              899  TGGCCA              
BlpI          462  GCTCAGC              GC^T[ACGT]AGC
              807  GCTGAGC             
BpmI          205  CTGGAG               CTGGAG(16/14)
              965  CTGGAG              
Bpu1102I      462  GCTCAGC              GC^T[ACGT]AGC
              807  GCTGAGC             
BseRI          63  CTCCTC               GAGGAG(10/8)
               22  GAGGAG              
BsmFI         227  GGGAC                GGGAC(10/14)
              437  GGGAC               
BsrBI          80  GAGCGG               CCGCTC(-3/-3)
              451  GAGCGG              
CelII         462  GCTCAGC              GC^T[ACGT]AGC
              807  GCTGAGC             
EaeI          758  TGGCCA               [CT]^GGCC[GA]
              899  TGGCCA              
MaeIII       1037  GTAAC                ^GT[ACGT]AC
             1046  GTGAC               
MluNI         758  TGGCCA               TGG^CCA
              899  TGGCCA              
MscI          758  TGGCCA               TGG^CCA
              899  TGGCCA              
MspA1I        115  CAGCGG               C[AC]G^C[GT]G
              421  CAGCTG              
PleI          490  GACTC                GAGTC(4/5)
              678  GACTC               
RsaI          240  GTAC                 GT^AC
             1116  GTAC                
TspRI         172  CAGTG                CA[GC]TG[ACGT][ACGT]^
              181  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AlwNI       BanII       BbvI        
BmyI        BsaI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
FauI        Fnu4HI      HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        MboI        MboII       
MnlI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PstI        Sau3AI      Sau96I      ScrFI       
SfcI        TaqI        TseI        TthHB8I     
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