Restriction map for pF1KB9705
 (Linear) (Six-base) MAPSORT of: KIBB9705.seq  Check: 8384  from: 1  to: 1005



KIBB9705.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AccI GT'mk_AC

Cuts at:      0     791    1005 
   Size:        791     214
 
Acc65I G'GTAC_C

Cuts at:      0     659    1005 
   Size:        659     346
 
AfeI AGC'GCT

Cuts at:      0     339     347    1005 
   Size:        339       8     658

  Fragments arranged by size: 

                 658     339       8
 
AflIII A'CryG_T

Cuts at:      0     482     782    1005 
   Size:        482     300     223

  Fragments arranged by size: 

                 482     300     223
 
AhdI GACnn_n'nnGTC

Cuts at:      0     603    1005 
   Size:        603     402
 
ApoI r'AATT_y

Cuts at:      0     871    1005 
   Size:        871     134
 
AscI GG'CGCG_CC

Cuts at:      0     265    1005 
   Size:        265     740
 
AvaI C'yCGr_G

Cuts at:      0     205     333    1005 
   Size:        205     128     672

  Fragments arranged by size: 

                 672     205     128
 
BanI G'GyrC_C

Cuts at:      0      28      37     522     659    1005 
   Size:         28       9     485     137     346

  Fragments arranged by size: 

                 485     346     137      28       9
 
BanII G_rGCy'C

Cuts at:      0     229     571    1005 
   Size:        229     342     434

  Fragments arranged by size: 

                 434     342     229
 
BbeI G_GCGC'C

Cuts at:      0      32      41    1005 
   Size:         32       9     964

  Fragments arranged by size: 

                 964      32       9
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     555    1005 
   Size:        555     450
 
Bme1580I G_kGCm'C

Cuts at:      0     298     587    1005 
   Size:        298     289     418

  Fragments arranged by size: 

                 418     298     289
 
BmgBI CAC'GTC

Cuts at:      0     483    1005 
   Size:        483     522
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     868    1005 
   Size:        326     542     137

  Fragments arranged by size: 

                 542     326     137
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    1005 
   Size:        412     593
 
BsaAI yAC'GTr

Cuts at:      0     582    1005 
   Size:        582     423
 
BsaHI Gr'CG_yC

Cuts at:      0      29      38      82    1005 
   Size:         29       9      44     923

  Fragments arranged by size: 

                 923      44      29       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     841     871    1005 
   Size:        841      30     134

  Fragments arranged by size: 

                 841     134      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      45      48    1005 
   Size:         45       3     957

  Fragments arranged by size: 

                 957      45       3
 
BseYI C'CCAG_C

Cuts at:      0     282     297     348     450    1005 
   Size:        282      15      51     102     555

  Fragments arranged by size: 

                 555     282     102      51      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     690    1005 
   Size:        690     315
 
BsiEI CG_ry'CG

Cuts at:      0     320    1005 
   Size:        320     685
 
BsmI GAATG_Cn'

Cuts at:      0     132     426    1005 
   Size:        132     294     579

  Fragments arranged by size: 

                 579     294     132
 
Bsp1286I G_dGCh'C

Cuts at:      0     229     298     571     587    1005 
   Size:        229      69     273      16     418

  Fragments arranged by size: 

                 418     273     229      69      16
 
BsrFI r'CCGG_y

Cuts at:      0      31     510    1005 
   Size:         31     479     495

  Fragments arranged by size: 

                 495     479      31
 
BssHII G'CGCG_C

Cuts at:      0     265    1005 
   Size:        265     740
 
BssSI C'ACGA_G

Cuts at:      0     634    1005 
   Size:        634     371
 
BtgI C'CryG_G

Cuts at:      0      96     464     650    1005 
   Size:         96     368     186     355

  Fragments arranged by size: 

                 368     355     186      96
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     419    1005 
   Size:        419     586
 
DraIII CAC_nnn'GTG

Cuts at:      0     405    1005 
   Size:        405     600
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     787    1005 
   Size:        787     218
 
EaeI y'GGCC_r

Cuts at:      0     317     512    1005 
   Size:        317     195     493

  Fragments arranged by size: 

                 493     317     195
 
EagI C'GGCC_G

Cuts at:      0     317    1005 
   Size:        317     688
 
EarI CTCTTCn'nnn_

Cuts at:      0      54     234     252     792     805     951    1005 
   Size:         54     180      18     540      13     146      54

  Fragments arranged by size: 

                 540     180     146      54      54      18      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     868    1005 
   Size:        326     542     137

  Fragments arranged by size: 

                 542     326     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     209     728    1005 
   Size:        209     519     277

  Fragments arranged by size: 

                 519     277     209
 
HaeII r_GCGC'y

Cuts at:      0      32      41     331     341     349    1005 
   Size:         32       9     290      10       8     656

  Fragments arranged by size: 

                 656     290      32      10       9       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     589     621     985    1005 
   Size:        589      32     364      20

  Fragments arranged by size: 

                 589     364      32      20
 
KasI G'GCGC_C

Cuts at:      0      28      37    1005 
   Size:         28       9     968

  Fragments arranged by size: 

                 968      28       9
 
KpnI G_GTAC'C

Cuts at:      0     663    1005 
   Size:        663     342
 
MslI CAynn'nnrTG

Cuts at:      0     481     649    1005 
   Size:        481     168     356

  Fragments arranged by size: 

                 481     356     168
 
MspA1I CmG'CkG

Cuts at:      0      98     160     181     316     322     386    1005 
   Size:         98      62      21     135       6      64     619

  Fragments arranged by size: 

                 619     135      98      64      62      21       6
 
NarI GG'CG_CC

Cuts at:      0      29      38    1005 
   Size:         29       9     967

  Fragments arranged by size: 

                 967      29       9
 
NcoI C'CATG_G

Cuts at:      0     650    1005 
   Size:        650     355
 
NotI GC'GGCC_GC

Cuts at:      0     317    1005 
   Size:        317     688
 
NspI r_CATG'y

Cuts at:      0     786    1005 
   Size:        786     219
 
PciI A'CATG_T

Cuts at:      0     782    1005 
   Size:        782     223
 
PfoI T'CCnGG_A

Cuts at:      0     667    1005 
   Size:        667     338
 
PmlI CAC'GTG

Cuts at:      0     582    1005 
   Size:        582     423
 
PpuMI rG'GwC_Cy

Cuts at:      0     209     728    1005 
   Size:        209     519     277

  Fragments arranged by size: 

                 519     277     209
 
PstI C_TGCA'G

Cuts at:      0     315     327     836    1005 
   Size:        315      12     509     169

  Fragments arranged by size: 

                 509     315     169      12
 
PvuII CAG'CTG

Cuts at:      0     386    1005 
   Size:        386     619
 
SacII CC_GC'GG

Cuts at:      0      99    1005 
   Size:         99     906
 
SanDI GG'GwC_CC

Cuts at:      0     728    1005 
   Size:        728     277
 
SapI GCTCTTCn'nnn_

Cuts at:      0     234    1005 
   Size:        234     771
 
SfcI C'TryA_G

Cuts at:      0     311     323     381     832    1005 
   Size:        311      12      58     451     173

  Fragments arranged by size: 

                 451     311     173      58      12
 
SfoI GGC'GCC

Cuts at:      0      30      39    1005 
   Size:         30       9     966

  Fragments arranged by size: 

                 966      30       9
 
SgrAI Cr'CCGG_yG

Cuts at:      0      31    1005 
   Size:         31     974
 
SmaI CCC'GGG

Cuts at:      0     207    1005 
   Size:        207     798
 
SmlI C'TyrA_G

Cuts at:      0     427    1005 
   Size:        427     578
 
StuI AGG'CCT

Cuts at:      0     491     640    1005 
   Size:        491     149     365

  Fragments arranged by size: 

                 491     365     149
 
StyI C'CwwG_G

Cuts at:      0     536     650     710    1005 
   Size:        536     114      60     295

  Fragments arranged by size: 

                 536     295     114      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     318    1005 
   Size:        318     687
 
TatI w'GTAC_w

Cuts at:      0     473     701    1005 
   Size:        473     228     304

  Fragments arranged by size: 

                 473     304     228
 
XmaI C'CCGG_G

Cuts at:      0     205    1005 
   Size:        205     800
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI   AflIII     AhdI     ApoI     AscI     AvaI
     BanI    BanII     BbeI    BciVI Bme1580I    BmgBI     BpmI    BpuEI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI     BsmI
 Bsp1286I    BsrFI   BssHII    BssSI     BtgI    BtgZI   DraIII     DrdI
     EaeI     EagI     EarI  Eco57MI EcoO109I    HaeII    Hin4I     KasI
     KpnI     MslI   MspA1I     NarI     NcoI     NotI     NspI     PciI
     PfoI     PmlI    PpuMI     PstI    PvuII    SacII    SanDI     SapI
     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII     AgeI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaWI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     MfeI     MluI     MmeI     MscI     NaeI     NdeI   NgoMIV     NheI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     SbfI     ScaI    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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