Restriction map for pF1KB9704
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspHI       AspI        AvrII       BamHI       BbeI        
BbrPI       BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiHKAI     BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstBI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DdeI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HaeII       Hin1I       HincII      HindII      
HindIII     HpaI        KasI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tsp509I     TspRI       Tth111I     Van91I      
XbaI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        671  GGTACC               G^GTACC
AlwI          390  GATCC                GGATC(4/5)
AlwNI         279  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          212  GGGCCC               GGGCC^C
Asp718I       671  GGTACC               G^GTACC
AviII         578  TGCGCA               TGC^GCA
BalI          275  TGGCCA               TGG^CCA
BbsI          624  GAAGAC               GAAGAC(2/6)
BcgI          830  CGAGCGGCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI           19  CTAG                 C^TAG
BpuAI         624  GAAGAC               GAAGAC(2/6)
BseRI         298  GAGGAG               GAGGAG(10/8)
BsmFI         611  GGGAC                GGGAC(10/14)
BspMI         270  GCAGGT               ACCTGC(4/8)
BsrDI         631  CATTGC               GCAATG(2/0)
BssSI         382  CACGAG               CACGAG(-5/-1)
BstAPI        701  GCAAGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        966  GGTAACC              G^GT[ACGT]ACC
BstPI         966  GGTAACC              G^GT[ACGT]ACC
DpnII         390  GATC                 ^GATC
EagI          543  CGGCCG               C^GGCCG
EarI          223  GAAGAG               CTCTTC(1/4)
EclXI         543  CGGCCG               C^GGCCG
Eco52I        543  CGGCCG               C^GGCCG
EcoO65I       966  GGTAACC              G^GT[ACGT]ACC
EcoT14I       473  CCAAGG               C^C[AT][AT]GG
FspI          578  TGCGCA               TGC^GCA
KpnI          671  GGTACC               GGTAC^C
Ksp632I       223  GAAGAG               CTCTTC(1/4)
KspI          788  CCGCGG               CCGC^GG
MaeI           19  CTAG                 C^TAG
MaeIII        967  GTAAC                ^GT[ACGT]AC
MboI          390  GATC                 ^GATC
MluNI         275  TGGCCA               TGG^CCA
MscI          275  TGGCCA               TGG^CCA
MseI          761  TTAA                 T^TAA
MslI          912  CACCAGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         390  GATC                 ^GATC
NotI          542  GCGGCCGC             GC^GGCCGC
NspI          836  GCATGC               [GA]CATG^[CT]
PaeR7I        508  CTCGAG               C^TCGAG
PpuMI         680  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          493  CTGCAG               CTGCA^G
PvuII         241  CAGCTG               CAG^CTG
SacII         788  CCGCGG               CCGC^GG
Sau3AI        390  GATC                 ^GATC
SmaI          355  CCCGGG               CCC^GGG
SphI          836  GCATGC               GCATG^C
StyI          473  CCAAGG               C^C[AT][AT]GG
TfiI           61  GATTC                G^A[AT]TC
Tru9I         761  TTAA                 T^TAA
XcmI          598  CCACGCACCATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          508  CTCGAG               C^TCGAG
XmaCI         355  CCCGGG               C^CCGGG
XmaI          355  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          570  GTAC                 GT^AC
              672  GTAC                
AvaI          355  CCCGGG               C^[CT]CG[GA]G
              508  CTCGAG              
AvaII         681  GGACC                G^G[AT]CC
              826  GGACC               
BanI          671  GGTACC               G^G[CT][GA]CC
              949  GGCACC              
BanII         212  GGGCCC               G[GA]GC[CT]^C
              853  GAGCCC              
BmyI          212  GGGCCC               G[AGT]GC[ACT]^C
              853  GAGCCC              
BsiEI         543  CGGCCG               CG[GA][CT]^CG
              780  CGACCG              
BsmBI         289  GAGACG               CGTCTC(1/5)
              301  GAGACG              
BsoBI         355  CCCGGG               C^[CT]CG[GA]G
              508  CTCGAG              
Bsp1286I      212  GGGCCC               G[AGT]GC[ACT]^C
              853  GAGCCC              
BsrI          714  ACTGG                ACTGG(1/-1)
              755  ACTGG               
BstF5I        450  CATCC                GGATG(2/0)
              957  CATCC               
BstXI          77  CCATACTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              816  CCAGGCCATTGG        
FokI          450  CATCC                GGATG(9/13)
              957  CATCC               
MboII         223  GAAGA                GAAGA(8/7)
              624  GAAGA               
RsaI          570  GTAC                 GT^AC
              672  GTAC                
SfcI          493  CTGCAG               C^T[GA][CT]AG
              676  CTACAG              
SmlI          454  CTTGAG               C^T[CT][GA]AG
              508  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BcnI        
BglI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsrBI       BsrFI       BssHII      BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       PleI        Sau96I      ScrFI       TaqI        
TseI        TthHB8I     
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