Restriction map for pF1KB9700
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BfrI        
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaI        BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HhaI        HinP1I      
HinfI       HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
MwoI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tth111I     
TthHB8I     Van91I      XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI          852  GCGG                 CCGC(-3/-1)
AcyI          286  GGCGTC               G[GA]^CG[CT]C
AflIII        736  ACATGT               A^C[GA][CT]GT
AspHI         109  GTGCTC               G[AT]GC[AT]^C
BamHI          33  GGATCC               G^GATCC
BanI          374  GGCACC               G^G[CT][GA]CC
BciVI         169  GTATCC               GTATCC(6/5)
BcnI          174  CCCGG                CC^[GC]GG
BglII         606  AGATCT               A^GATCT
BmyI          109  GTGCTC               G[AGT]GC[ACT]^C
BsaHI         286  GGCGTC               G[GA]^CG[CT]C
BsaWI         788  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       109  GTGCTC               G[AT]GC[AT]^C
Bsp1286I      109  GTGCTC               G[AGT]GC[ACT]^C
BspHI         741  TCATGA               T^CATGA
BspLU11I      736  ACATGT               A^CATGT
BsrI          388  CCAGT                ACTGG(1/-1)
Cfr13I         48  GGCCC                G^G[ACGT]CC
DraI          481  TTTAAA               TTT^AAA
DraII          47  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        358  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          157  CCATGG               C^C[GA][CT]GG
EcoO109I       47  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         542  GAATTC               G^AATTC
HgaI          287  GCGTC                GACGC(5/10)
Hin1I         286  GGCGTC               G[GA]^CG[CT]C
HincII        572  GTCAAC               GT[CT]^[GA]AC
HindII        572  GTCAAC               GT[CT]^[GA]AC
HindIII       672  AAGCTT               A^AGCTT
HphI          805  GGTGA                GGTGA(8/7)
MaeIII        449  GTCAC                ^GT[ACGT]AC
NciI          174  CCCGG                CC^[GC]GG
NcoI          157  CCATGG               C^CATGG
NdeI          828  CATATG               CA^TATG
NspI          736  ACATGT               [GA]CATG^[CT]
RcaI          741  TCATGA               T^CATGA
SapI          151  GAAGAGC              GCTCTTC(1/4)
Sau96I         48  GGCCC                G^G[ACGT]CC
SfaNI         277  GCATC                GCATC(5/9)
SfcI          206  CTACAG               C^T[GA][CT]AG
Tsp45I        449  GTCAC                ^GT[GC]AC
XbaI          138  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Asp700I       501  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              669  GAAAAGCTTC          
BbvI          810  GCAGC                GCAGC(8/12)
              429  GCTGC               
BfaI           97  CTAG                 C^TAG
              139  CTAG                
BsmAI          82  GAGAC                GTCTC(1/5)
              252  GTCTC               
BstNI          12  CCAGG                CC^[AT]GG
              416  CCTGG               
BstYI          33  GGATCC               [GA]^GATC[CT]
              606  AGATCT              
EarI          151  GAAGAG               CTCTTC(1/4)
              553  GAAGAG              
EcoRII         12  CCAGG                ^CC[AT]GG
              416  CCTGG               
EcoT14I       157  CCATGG               C^C[AT][AT]GG
              633  CCAAGG              
Fnu4HI        429  GCTGC                GC^[ACGT]GC
              810  GCAGC               
HaeIII         48  GGCC                 GG^CC
              303  GGCC                
ItaI          429  GCTGC                GC^[ACGT]GC
              810  GCAGC               
Ksp632I       151  GAAGAG               CTCTTC(1/4)
              553  GAAGAG              
MaeI           97  CTAG                 C^TAG
              139  CTAG                
MflI           33  GGATCC               [GA]^GATC[CT]
              606  AGATCT              
MnlI           51  CCTC                 CCTC(7/6)
              588  GAGG                
MvaI           12  CCAGG                CC^[AT]GG
              416  CCTGG               
NlaIV          33  GGATCC               GG[ACGT]^[ACGT]CC
              374  GGCACC              
StyI          157  CCATGG               C^C[AT][AT]GG
              633  CCAAGG              
TseI          429  GCTGC                G^C[AT]GC
              810  GCAGC               
XhoII          33  GGATCC               [GA]^GATC[CT]
              606  AGATCT              
XmnI          501  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              669  GAAAAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BsaJI       
BssKI       Cac8I       DdeI        DpnII       HapII       
HpaII       MboI        MboII       MseI        MslI        
MspI        NdeII       NlaIII      Sau3AI      ScrFI       
Tru9I       Tsp509I     TspRI       
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