Restriction map for pF1KB9696
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiHKAI     BsiWI       
BsmAI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DdeI        DraI        DraIII      
DrdI        Eam1105I    EarI        Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     MaeI        
MamI        MfeI        MluI        MroI        MseI        
MslI        MunI        NcoI        NdeI        NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          478  GTCTAC               GT^[AC][GT]AC
AvaII         455  GGACC                G^G[AT]CC
BalI          752  TGGCCA               TGG^CCA
BbrPI         148  CACGTG               CAC^GTG
BglII         596  AGATCT               A^GATCT
BsaAI         148  CACGTG               [CT]AC^GT[GA]
BsiEI         422  CGGCCG               CG[GA][CT]^CG
BsmFI         731  GTCCC                GGGAC(10/14)
BsmI          271  GCATTC               GAATGC(1/-1)
BspMI         588  GCAGGT               ACCTGC(4/8)
BsrI          212  ACTGG                ACTGG(1/-1)
BstXI         743  CCAGCCTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         596  AGATCT               [GA]^GATC[CT]
DpnII         597  GATC                 ^GATC
DsaI          239  CCGCGG               C^C[GA][CT]GG
EagI          422  CGGCCG               C^GGCCG
EclXI         422  CGGCCG               C^GGCCG
Eco52I        422  CGGCCG               C^GGCCG
HgaI          390  GACGC                GACGC(5/10)
HphI          591  GGTGA                GGTGA(8/7)
KspI          239  CCGCGG               CCGC^GG
MaeII         149  ACGT                 A^CGT
MaeIII        530  GTTAC                ^GT[ACGT]AC
MboI          597  GATC                 ^GATC
MflI          596  AGATCT               [GA]^GATC[CT]
MluNI         752  TGGCCA               TGG^CCA
MscI          752  TGGCCA               TGG^CCA
NdeII         597  GATC                 ^GATC
PflMI         492  CCAACGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          450  GACTC                GAGTC(4/5)
PmaCI         148  CACGTG               CAC^GTG
PmlI          148  CACGTG               CAC^GTG
SacII         239  CCGCGG               CCGC^GG
Sau3AI        597  GATC                 ^GATC
SfaNI         405  GATGC                GCATC(5/9)
TfiI           28  GATTC                G^A[AT]TC
TspRI         579  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        492  CCAACGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           62  CCAGCCTCGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         596  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BglI          178  GCCTGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              309  GCCCCCTGGGC         
BseRI         759  CTCCTC               GAGGAG(10/8)
              781  GAGGAG              
BstF5I        713  CATCC                GGATG(2/0)
              404  GGATG               
EcoT14I       203  CCAAGG               C^C[AT][AT]GG
              458  CCAAGG              
FokI          713  CATCC                GGATG(9/13)
              404  GGATG               
HinfI          28  GATTC                G^A[ACGT]TC
              450  GACTC               
MboII         594  GAAGA                GAAGA(8/7)
              642  GAAGA               
MspA1I        239  CCGCGG               C[AC]G^C[GT]G
              441  CAGCGG              
NaeI          156  GCCGGC               GCC^GGC
              303  GCCGGC              
NgoMIV        156  GCCGGC               G^CCGGC
              303  GCCGGC              
StyI          203  CCAAGG               C^C[AT][AT]GG
              458  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
ApaI        AvaI        BanI        BanII       BbeI        
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BsiYI       BslI        BsoBI       Bsp1286I    
BsrFI       BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HpaII       ItaI        KasI        MnlI        MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NlaIV       RsaI        Sau96I      ScrFI       SfoI        
SmaI        TseI        XmaCI       XmaI        
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