Restriction map for pF1KB9696
 (Linear) (Six-base) MAPSORT of: KIBB9696.seq  Check: 6875  from: 1  to: 795



KIBB9696.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     579     795 
   Size:        579     216
 
AccI GT'mk_AC

Cuts at:      0     479     795 
   Size:        479     316
 
ApaI G_GGCC'C

Cuts at:      0      79     238     281     320     355     795 
   Size:         79     159      43      39      35     440

  Fragments arranged by size: 

                 440     159      79      43      39      35
 
AvaI C'yCGr_G

Cuts at:      0     231     324     346     354     795 
   Size:        231      93      22       8     441

  Fragments arranged by size: 

                 441     231      93      22       8
 
BanI G'GyrC_C

Cuts at:      0     111     186     300     306     795 
   Size:        111      75     114       6     489

  Fragments arranged by size: 

                 489     114     111      75       6
 
BanII G_rGCy'C

Cuts at:      0      79     238     281     320     355     578     795 
   Size:         79     159      43      39      35     223     217

  Fragments arranged by size: 

                 223     217     159      79      43      39      35
 
BbeI G_GCGC'C

Cuts at:      0     115     190     310     795 
   Size:        115      75     120     485

  Fragments arranged by size: 

                 485     120     115      75
 
BglI GCCn_nnn'nGGC

Cuts at:      0     184     315     795 
   Size:        184     131     480

  Fragments arranged by size: 

                 480     184     131
 
BglII A'GATC_T

Cuts at:      0     596     795 
   Size:        596     199
 
Bme1580I G_kGCm'C

Cuts at:      0      79     238     281     320     355     361     795 
   Size:         79     159      43      39      35       6     434

  Fragments arranged by size: 

                 434     159      79      43      39      35       6
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     222     795 
   Size:        222     573
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     269     520     526     795 
   Size:        120     149     251       6     269

  Fragments arranged by size: 

                 269     251     149     120       6
 
BsaAI yAC'GTr

Cuts at:      0     150     795 
   Size:        150     645
 
BsaHI Gr'CG_yC

Cuts at:      0     112     187     307     391     795 
   Size:        112      75     120      84     404

  Fragments arranged by size: 

                 404     120     112      84      75
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     499     529     747     777     795 
   Size:        499      30     218      30      18

  Fragments arranged by size: 

                 499     218      30      30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     750     795 
   Size:        750      45
 
BseYI C'CCAG_C

Cuts at:      0      48     258     702     733     795 
   Size:         48     210     444      31      62

  Fragments arranged by size: 

                 444     210      62      48      31
 
BsiEI CG_ry'CG

Cuts at:      0     425     795 
   Size:        425     370
 
BsmI GAATG_Cn'

Cuts at:      0     271     795 
   Size:        271     524
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     238     281     320     355     361     578     795 
   Size:         79     159      43      39      35       6     217     217

  Fragments arranged by size: 

                 217     217     159      79      43      39      35       6
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     579     795 
   Size:        579     216
 
BsrFI r'CCGG_y

Cuts at:      0     156     303     611     795 
   Size:        156     147     308     184

  Fragments arranged by size: 

                 308     184     156     147
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     750     795 
   Size:        750      45
 
BstYI r'GATC_y

Cuts at:      0     596     795 
   Size:        596     199
 
BtgI C'CryG_G

Cuts at:      0     239     795 
   Size:        239     556
 
EaeI y'GGCC_r

Cuts at:      0     422     695     752     795 
   Size:        422     273      57      43

  Fragments arranged by size: 

                 422     273      57      43
 
EagI C'GGCC_G

Cuts at:      0     422     795 
   Size:        422     373
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     402     561     795 
   Size:        402     159     234

  Fragments arranged by size: 

                 402     234     159
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     269     520     526     795 
   Size:        120     149     251       6     269

  Fragments arranged by size: 

                 269     251     149     120       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     235     278     351     723     795 
   Size:        235      43      73     372      72

  Fragments arranged by size: 

                 372     235      73      72      43
 
HaeII r_GCGC'y

Cuts at:      0     115     190     310     795 
   Size:        115      75     120     485

  Fragments arranged by size: 

                 485     120     115      75
 
KasI G'GCGC_C

Cuts at:      0     111     186     306     795 
   Size:        111      75     120     489

  Fragments arranged by size: 

                 489     120     111      75
 
MscI TGG'CCA

Cuts at:      0     754     795 
   Size:        754      41
 
MspA1I CmG'CkG

Cuts at:      0     241     443     795 
   Size:        241     202     352

  Fragments arranged by size: 

                 352     241     202
 
NaeI GCC'GGC

Cuts at:      0     158     305     795 
   Size:        158     147     490

  Fragments arranged by size: 

                 490     158     147
 
NarI GG'CG_CC

Cuts at:      0     112     187     307     795 
   Size:        112      75     120     488

  Fragments arranged by size: 

                 488     120     112      75
 
NgoMIV G'CCGG_C

Cuts at:      0     156     303     795 
   Size:        156     147     492

  Fragments arranged by size: 

                 492     156     147
 
PasI CC'CwG_GG

Cuts at:      0     313     717     795 
   Size:        313     404      78

  Fragments arranged by size: 

                 404     313      78
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     498     795 
   Size:        498     297
 
PmlI CAC'GTG

Cuts at:      0     150     795 
   Size:        150     645
 
PspOMI G'GGCC_C

Cuts at:      0      75     234     277     316     351     795 
   Size:         75     159      43      39      35     444

  Fragments arranged by size: 

                 444     159      75      43      39      35
 
SacII CC_GC'GG

Cuts at:      0     242     795 
   Size:        242     553
 
SfoI GGC'GCC

Cuts at:      0     113     188     308     795 
   Size:        113      75     120     487

  Fragments arranged by size: 

                 487     120     113      75
 
SmaI CCC'GGG

Cuts at:      0     233     326     356     795 
   Size:        233      93      30     439

  Fragments arranged by size: 

                 439     233      93      30
 
SrfI GCCC'GGGC

Cuts at:      0     233     326     356     795 
   Size:        233      93      30     439

  Fragments arranged by size: 

                 439     233      93      30
 
StyI C'CwwG_G

Cuts at:      0     203     458     795 
   Size:        203     255     337

  Fragments arranged by size: 

                 337     255     203
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     747     779     795 
   Size:        747      32      16

  Fragments arranged by size: 

                 747      32      16
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      69     795 
   Size:         69     726
 
XmaI C'CCGG_G

Cuts at:      0     231     324     354     795 
   Size:        231      93      30     441

  Fragments arranged by size: 

                 441     231      93      30
 

 Enzymes that do cut:

     AarI     AccI     ApaI     AvaI     BanI    BanII     BbeI     BglI
    BglII Bme1580I     BmrI     BpmI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI    BsiEI     BsmI Bsp1286I    BspMI    BsrFI    BstXI    BstYI
     BtgI     EaeI     EagI     EciI  Eco57MI EcoO109I    HaeII     KasI
     MscI   MspA1I     NaeI     NarI   NgoMIV     PasI    PflMI     PmlI
   PspOMI    SacII     SfoI     SmaI     SrfI     StyI     TstI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI     BsgI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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