Restriction map for pF1KB9695
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AvrII       BalI        
BamHI       BbrPI       BbsI        BciVI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BsrI        
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        Eam1105I    
Eco47III    EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfcI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AlwI          774  GGATC                GGATC(4/5)
AocI          755  CCTCAGG              CC^T[ACGT]AGG
ApaI          205  GGGCCC               GGGCC^C
AspHI         714  GTGCTC               G[AT]GC[AT]^C
BanII         205  GGGCCC               G[GA]GC[CT]^C
BbvCI         704  CCTCAGC              CCTCAGC(-5/-2)
BcgI          530  GCATCGAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          207  GCCCCCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         575  AGATCT               A^GATCT
BpmI          484  CTGGAG               CTGGAG(16/14)
BsaI          121  GGTCTC               GGTCTC(1/5)
BsgI          115  CTGCAC               GTGCAG(16/14)
BsiHKAI       714  GTGCTC               G[AT]GC[AT]^C
BsmFI         516  GTCCC                GGGAC(10/14)
BspMI         567  GCAGGT               ACCTGC(4/8)
BsrFI         655  GCCGGT               [GA]^CCGG[CT]
BssHII        145  GCGCGC               G^CGCGC
BstAPI        108  GCACGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        755  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        655  GCCGGT               [GA]^CCGG[CT]
DraII         205  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI          329  CGGCCG               [CT]^GGCC[GA]
EagI          329  CGGCCG               C^GGCCG
EarI           82  CTCTTC               CTCTTC(1/4)
EclXI         329  CGGCCG               C^GGCCG
Eco52I        329  CGGCCG               C^GGCCG
Eco81I        755  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      205  GGGCCCC              [GA]G^G[ACGT]CC[CT]
HgaI          182  GCGTC                GACGC(5/10)
Ksp632I        82  CTCTTC               CTCTTC(1/4)
MaeII         525  ACGT                 A^CGT
MslI          402  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI          539  TCGCGA               TCG^CGA
PleI           22  GACTC                GAGTC(4/5)
SapI           81  GCTCTTC              GCTCTTC(1/4)
ScaI          266  AGTACT               AGT^ACT
SfiI          206  GGCCCCCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          235  CTCAAG               C^T[CT][GA]AG
TaqI          533  TCGA                 T^CGA
TfiI          552  GAATC                G^A[AT]TC
Tsp509I       734  AATT                 ^AATT
TspRI         397  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       533  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI           57  GGCGCC               G[GA]^CG[CT]C
              133  GGCGCC              
AfaI          267  GTAC                 GT^AC
              510  GTAC                
AviII         449  TGCGCA               TGC^GCA
              691  TGCGCA              
BbeI           57  GGCGCC               GGCGC^C
              133  GGCGCC              
BfaI           28  CTAG                 C^TAG
              478  CTAG                
BsaHI          57  GGCGCC               G[GA]^CG[CT]C
              133  GGCGCC              
BseRI         702  CTCCTC               GAGGAG(10/8)
              753  CTCCTC              
BsiEI         329  CGGCCG               CG[GA][CT]^CG
              768  CGACCG              
BsiYI         250  CCACCCTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              752  CCTCCTCAGGG         
BslI          250  CCACCCTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              752  CCTCCTCAGGG         
BsmAI         122  GTCTC                GTCTC(1/5)
              744  GTCTC               
BsrBI          79  CCGCTC               CCGCTC(-3/-3)
              175  CCGCTC              
BstF5I        446  GGATG                GGATG(2/0)
              723  GGATG               
BstYI         575  AGATCT               [GA]^GATC[CT]
              774  GGATCT              
DdeI          705  CTCAG                C^T[ACGT]AG
              756  CTCAG               
FokI          446  GGATG                GGATG(9/13)
              723  GGATG               
FspI          449  TGCGCA               TGC^GCA
              691  TGCGCA              
HaeII          57  GGCGCC               [GA]GCGC^[CT]
              133  GGCGCC              
Hin1I          57  GGCGCC               G[GA]^CG[CT]C
              133  GGCGCC              
HinfI          22  GACTC                G^A[ACGT]TC
              552  GAATC               
KasI           57  GGCGCC               G^GCGCC
              133  GGCGCC              
MaeI           28  CTAG                 C^TAG
              478  CTAG                
MaeIII        184  GTCAC                ^GT[ACGT]AC
              784  GTCAC               
MflI          575  AGATCT               [GA]^GATC[CT]
              774  GGATCT              
NarI           57  GGCGCC               GG^CGCC
              133  GGCGCC              
NlaIII        202  CATG                 CATG^
              741  CATG                
RsaI          267  GTAC                 GT^AC
              510  GTAC                
SfoI           57  GGCGCC               GGC^GCC
              133  GGCGCC              
SmaI          211  CCCGGG               CCC^GGG
              310  CCCGGG              
Tsp45I        184  GTCAC                ^GT[GC]AC
              784  GTCAC               
XhoII         575  AGATCT               [GA]^GATC[CT]
              774  GGATCT              
XmaCI         211  CCCGGG               C^CCGGG
              310  CCCGGG              
XmaI          211  CCCGGG               C^CCGGG
              310  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        BanI        
BbvI        BcnI        BmyI        BsaJI       BsoBI       
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DpnII       EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIV       PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
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