Restriction map for
pF1KB9695
(Circular) (Six-base) MAPSORT of: pF1KB9695.seq Check: 9262 from: 1 to: 3905
pF1KB9695 3905 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 666 666
Size: 3905
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1730 2162 2909 3177 1730
Size: 432 747 268 2458
Fragments arranged by size:
2458 747 432 268
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 571 598 571
Size: 27 3878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 586 618 586
Size: 32 3873
AlwNI CAG_nnn'CTG
Cuts at: 1210 2777 1210
Size: 1567 2338
ApaI G_GGCC'C
Cuts at: 317 317
Size: 3905
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 909 3258 3514 909
Size: 2349 256 1300
Fragments arranged by size:
2349 1300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 169 319 418 925 169
Size: 150 99 507 3149
Fragments arranged by size:
3149 507 150 99
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 165 241 760 921 1335 1583 1618 165
Size: 76 519 161 414 248 35 2452
Fragments arranged by size:
2452 519 414 248 161 76 35
BanII G_rGCy'C
Cuts at: 317 919 1949 317
Size: 602 1030 2273
Fragments arranged by size:
2273 1030 602
BbeI G_GCGC'C
Cuts at: 169 245 1339 1587 169
Size: 76 1094 248 2487
Fragments arranged by size:
2487 1094 248 76
BbvCI CC'TCA_GC
Cuts at: 813 813
Size: 3905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 627 661 90
Size: 34 503 34 3334
Fragments arranged by size:
3334 503 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 1425 1425
Size: 3905
BglI GCCn_nnn'nGGC
Cuts at: 321 321
Size: 3905
BglII A'GATC_T
Cuts at: 683 1420 3457 683
Size: 737 2037 1131
Fragments arranged by size:
2037 1131 737
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 209 283 317 1530 1623 2679 209
Size: 74 34 1213 93 1056 1435
Fragments arranged by size:
1435 1213 1056 93 74 34
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1198 1533 3677 1117
Size: 81 335 2144 1345
Fragments arranged by size:
2144 1345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 3905
Bpu10I CC'TnA_GC
Cuts at: 813 813
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1081 1960 2452 2750 328
Size: 753 879 492 298 1483
Fragments arranged by size:
1483 879 753 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 235 3609 235
Size: 3374 531
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 1419 3456 6
Size: 1413 2037 455
Fragments arranged by size:
2037 1413 455
BsaHI Gr'CG_yC
Cuts at: 166 242 1336 1584 2286 3782 166
Size: 76 1094 248 702 1496 289
Fragments arranged by size:
1496 1094 702 289 248 76
BsaWI w'CCGG_w
Cuts at: 1094 1615 2293 2567 2714 3125 1094
Size: 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 457 487 586 616 879 909 457
Size: 30 99 30 263 30 3453
Fragments arranged by size:
3453 263 99 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 801 852 801
Size: 51 3854
BseYI C'CCAG_C
Cuts at: 262 541 1330 2088 2665 3161 262
Size: 279 789 758 577 496 1006
Fragments arranged by size:
1006 789 758 577 496 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 208
Size: 3905
BsiEI CG_ry'CG
Cuts at: 104 440 879 1447 1493 2701 104
Size: 336 439 568 46 1208 1308
Fragments arranged by size:
1308 1208 568 439 336 46
BsiHKAI G_wGCw'C
Cuts at: 826 919 1697 1887 2679 826
Size: 93 778 190 792 2052
Fragments arranged by size:
2052 792 778 190 93
Bsp1286I G_dGCh'C
Cuts at: 209 283 317 826 919 1530 1623 1697 1887
Size: 74 34 509 93 611 93 74 190
Cuts at: 1887 1949 2679 209
Size: 62 730 1435
Fragments arranged by size:
1435 730 611 509 190 93 93 74
74 62 34
BspEI T'CCGG_A
Cuts at: 3125 3125
Size: 3905
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 666 955 1169 1471 1852 666
Size: 289 214 302 381 2719
Fragments arranged by size:
2719 381 302 289 214
BsrBI CCG'CTC
Cuts at: 50 189 285 2197 2251 3735 3900 50
Size: 139 96 1912 54 1484 165 55
Fragments arranged by size:
1912 1484 165 139 96 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 763 1903 2084 2293 763
Size: 1140 181 209 2375
Fragments arranged by size:
2375 1140 209 181
BssHII G'CGCG_C
Cuts at: 253 253
Size: 3905
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 222 1282 222
Size: 1060 2845
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstYI r'GATC_y
Cuts at: 1 683 882 930 1397 1420 1755 2001 3013
Size: 682 199 48 467 23 335 246 1012
Cuts at: 3013 3099 3111 3457 1
Size: 86 12 346 449
Fragments arranged by size:
1012 682 467 449 346 335 246 199
86 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 864 864
Size: 3905
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 788 1855 2036 2199 788
Size: 1067 181 163 2494
Fragments arranged by size:
2494 1067 181 163
BtsI GCAGTG_nn'
Cuts at: 504 1148 504
Size: 644 3261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 723 758 723
Size: 35 3870
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 437 1490 1664 2055 2082 3427 437
Size: 1053 174 391 27 1345 915
Fragments arranged by size:
1345 1053 915 391 174 27
EagI C'GGCC_G
Cuts at: 437 1490 437
Size: 1053 2852
EarI CTCTTCn'nnn_
Cuts at: 196 1928 2138 196
Size: 1732 210 1963
Fragments arranged by size:
1963 1732 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1730 2162 2909 3177 613
Size: 1117 432 747 268 1341
Fragments arranged by size:
1341 1117 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 314 1044 314
Size: 730 3175
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 369 1924 369
Size: 1555 2350
FspI TGC'GCA
Cuts at: 559 801 1686 559
Size: 242 885 2778
Fragments arranged by size:
2778 885 242
FspAI rTGC'GCAy
Cuts at: 559 801 559
Size: 242 3663
HaeII r_GCGC'y
Cuts at: 169 245 1339 1587 2609 3588 169
Size: 76 1094 248 1022 979 486
Fragments arranged by size:
1094 1022 979 486 248 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 511 543 1747 1777 1779 1809 511
Size: 32 1204 30 2 30 2607
Fragments arranged by size:
2607 1204 32 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 165 241 1335 1583 165
Size: 76 1094 248 2487
Fragments arranged by size:
2487 1094 248 76
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2576 2760 2576
Size: 184 3721
MscI TGG'CCA
Cuts at: 1666 1666
Size: 3905
MslI CAynn'nnrTG
Cuts at: 514 1894 2021 514
Size: 1380 127 2398
Fragments arranged by size:
2398 1380 127
MspA1I CmG'CkG
Cuts at: 306 445 483 537 582 1016 1330 1690 2703
Size: 139 38 54 45 434 314 360 1013
Cuts at: 2703 2948 306
Size: 245 1263
Fragments arranged by size:
1263 1013 434 360 314 245 139 54
45 38
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 3905
NarI GG'CG_CC
Cuts at: 166 242 1336 1584 166
Size: 76 1094 248 2487
Fragments arranged by size:
2487 1094 248 76
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 3905
NruI TCG'CGA
Cuts at: 649 649
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PasI CC'CwG_GG
Cuts at: 390 390
Size: 3905
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 586 618 3044 3076 586
Size: 32 2426 32 1415
Fragments arranged by size:
2426 1415 32 32
PspOMI G'GGCC_C
Cuts at: 313 313
Size: 3905
PstI C_TGCA'G
Cuts at: 952 1637 952
Size: 685 3220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 306 537 582 1330 1690 306
Size: 231 45 748 360 2521
Fragments arranged by size:
2521 748 360 231 45
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 196 1928 2138 196
Size: 1732 210 1963
Fragments arranged by size:
1963 1732 210
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 376 3422 376
Size: 3046 859
SfcI C'TryA_G
Cuts at: 33 948 1633 2626 2817 33
Size: 915 685 993 191 1121
Fragments arranged by size:
1121 993 915 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 321 321
Size: 3905
SfoI GGC'GCC
Cuts at: 167 243 1337 1585 167
Size: 76 1094 248 2487
Fragments arranged by size:
2487 1094 248 76
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 321 420 927 321
Size: 99 507 3299
Fragments arranged by size:
3299 507 99
SmlI C'TyrA_G
Cuts at: 343 1060 1975 2467 2729 343
Size: 717 915 492 262 1519
Fragments arranged by size:
1519 915 717 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
StyI C'CwwG_G
Cuts at: 1039 1393 1039
Size: 354 3551
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1488 1621 2080 2289 1488
Size: 133 459 209 3104
Fragments arranged by size:
3104 459 209 133
TatI w'GTAC_w
Cuts at: 374 3420 374
Size: 3046 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1400 1400
Size: 3905
XmaI C'CCGG_G
Cuts at: 319 418 925 319
Size: 99 507 3299
Fragments arranged by size:
3299 507 99
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NruI NspI
PasI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I BtgI ClaI DraIII EcoNI EcoRV
FseI HindIII HpaI MfeI NcoI NdeI NheI NotI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI