Restriction map for pF1KB9691
 (Linear) (Six-base) MAPSORT of: KIBB9691.seq  Check: 9876  from: 1  to: 690



KIBB9691.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     359     690 
   Size:        359     331
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     427     459     690 
   Size:        427      32     231

  Fragments arranged by size: 

                 427     231      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     382     426     690 
   Size:        382      44     264

  Fragments arranged by size: 

                 382     264      44
 
AvaI C'yCGr_G

Cuts at:      0     268     598     690 
   Size:        268     330      92

  Fragments arranged by size: 

                 330     268      92
 
BanI G'GyrC_C

Cuts at:      0     127     133     217     295     470     604     690 
   Size:        127       6      84      78     175     134      86

  Fragments arranged by size: 

                 175     134     127      86      84      78       6
 
BanII G_rGCy'C

Cuts at:      0     193     275     690 
   Size:        193      82     415

  Fragments arranged by size: 

                 415     193      82
 
BbeI G_GCGC'C

Cuts at:      0     131     137     221     299     474     608     690 
   Size:        131       6      84      78     175     134      82

  Fragments arranged by size: 

                 175     134     131      84      82      78       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     365     683     690 
   Size:        365     318       7

  Fragments arranged by size: 

                 365     318       7
 
BbvCI CC'TCA_GC

Cuts at:      0     338     690 
   Size:        338     352
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     267     301     468     502     690 
   Size:        267      34     167      34     188

  Fragments arranged by size: 

                 267     188     167      34      34
 
BglII A'GATC_T

Cuts at:      0     524     690 
   Size:        524     166
 
Bme1580I G_kGCm'C

Cuts at:      0     614     690 
   Size:        614      76
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     454     690 
   Size:        448       6     236

  Fragments arranged by size: 

                 448     236       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     338     690 
   Size:        338     352
 
BsaBI GATnn'nnATC

Cuts at:      0     523     690 
   Size:        523     167
 
BsaHI Gr'CG_yC

Cuts at:      0     128     134     218     224     296     471     605     690 
   Size:        128       6      84       6      72     175     134      85

  Fragments arranged by size: 

                 175     134     128      85      84      72       6       6
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     427     457     690 
   Size:        427      30     233

  Fragments arranged by size: 

                 427     233      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     667     690 
   Size:        667      23
 
BsiEI CG_ry'CG

Cuts at:      0     149     690 
   Size:        149     541
 
Bsp1286I G_dGCh'C

Cuts at:      0     193     275     614     690 
   Size:        193      82     339      76

  Fragments arranged by size: 

                 339     193      82      76
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     245     690 
   Size:        245     445
 
BsrFI r'CCGG_y

Cuts at:      0     130     690 
   Size:        130     560
 
BssHII G'CGCG_C

Cuts at:      0     320     690 
   Size:        320     370
 
BstYI r'GATC_y

Cuts at:      0     524     690 
   Size:        524     166
 
BtgI C'CryG_G

Cuts at:      0     635     690 
   Size:        635      55
 
BtsI GCAGTG_nn'

Cuts at:      0     285     626     690 
   Size:        285     341      64

  Fragments arranged by size: 

                 341     285      64
 
EarI CTCTTCn'nnn_

Cuts at:      0     665     690 
   Size:        665      25
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     359     448     454     690 
   Size:        359      89       6     236

  Fragments arranged by size: 

                 359     236      89       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     379     690 
   Size:        379     311
 
HaeII r_GCGC'y

Cuts at:      0     131     137     221     299     474     516     608     690 
   Size:        131       6      84      78     175      42      92      82

  Fragments arranged by size: 

                 175     131      92      84      82      78      42       6
            
 
HincII GTy'rAC

Cuts at:      0     171     690 
   Size:        171     519
 
KasI G'GCGC_C

Cuts at:      0     127     133     217     295     470     604     690 
   Size:        127       6      84      78     175     134      86

  Fragments arranged by size: 

                 175     134     127      86      84      78       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     690 
   Size:        604      86
 
MspA1I CmG'CkG

Cuts at:      0     637     690 
   Size:        637      53
 
NaeI GCC'GGC

Cuts at:      0     132     690 
   Size:        132     558
 
NarI GG'CG_CC

Cuts at:      0     128     134     218     296     471     605     690 
   Size:        128       6      84      78     175     134      85

  Fragments arranged by size: 

                 175     134     128      85      84      78       6
 
NgoMIV G'CCGG_C

Cuts at:      0     130     690 
   Size:        130     560
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     426     690 
   Size:        426     264
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     427     459     690 
   Size:        427      32     231

  Fragments arranged by size: 

                 427     231      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     379     690 
   Size:        379     311
 
PshAI GACnn'nnGTC

Cuts at:      0     575     690 
   Size:        575     115
 
PstI C_TGCA'G

Cuts at:      0     280     690 
   Size:        280     410
 
SacII CC_GC'GG

Cuts at:      0     638     690 
   Size:        638      52
 
SfcI C'TryA_G

Cuts at:      0     276     690 
   Size:        276     414
 
SfoI GGC'GCC

Cuts at:      0     129     135     219     297     472     606     690 
   Size:        129       6      84      78     175     134      84

  Fragments arranged by size: 

                 175     134     129      84      84      78       6
 
SgrAI Cr'CCGG_yG

Cuts at:      0     130     690 
   Size:        130     560
 
SmaI CCC'GGG

Cuts at:      0     270     690 
   Size:        270     420
 
XmaI C'CCGG_G

Cuts at:      0     268     690 
   Size:        268     422
 
XmnI GAAnn'nnTTC

Cuts at:      0     442     690 
   Size:        442     248
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BglII Bme1580I     BpmI   Bpu10I    BsaBI    BsaHI
    BsaXI    BseRI    BsiEI Bsp1286I    BspMI    BsrFI   BssHII    BstYI
     BtgI     BtsI     EarI  Eco57MI EcoO109I    HaeII   HincII     KasI
     MmeI   MspA1I     NaeI     NarI   NgoMIV    PflMI     PpiI    PpuMI
    PshAI     PstI    SacII     SfcI     SfoI    SgrAI     SmaI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaWI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI
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