Restriction map for pF1KB9690
 (Linear) (Six-base) MAPSORT of: KIBB9690.seq  Check: 809  from: 1  to: 678



KIBB9690.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AatII G_ACGT'C

Cuts at:      0      77     678 
   Size:         77     601
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     678 
   Size:        424     254
 
AfeI AGC'GCT

Cuts at:      0     310     418     678 
   Size:        310     108     260

  Fragments arranged by size: 

                 310     260     108
 
AgeI A'CCGG_T

Cuts at:      0     295     678 
   Size:        295     383
 
ApaI G_GGCC'C

Cuts at:      0     110     678 
   Size:        110     568
 
AvaI C'yCGr_G

Cuts at:      0     103     214     302     678 
   Size:        103     111      88     376

  Fragments arranged by size: 

                 376     111     103      88
 
BanI G'GyrC_C

Cuts at:      0      84     292     678 
   Size:         84     208     386

  Fragments arranged by size: 

                 386     208      84
 
BanII G_rGCy'C

Cuts at:      0     110     597     678 
   Size:        110     487      81

  Fragments arranged by size: 

                 487     110      81
 
BbeI G_GCGC'C

Cuts at:      0      88     678 
   Size:         88     590
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     176     678 
   Size:        176     502
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     293     327     678 
   Size:        293      34     351

  Fragments arranged by size: 

                 351     293      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     105     407     496     678 
   Size:        105     302      89     182

  Fragments arranged by size: 

                 302     182     105      89
 
BlpI GC'TnA_GC

Cuts at:      0     349     678 
   Size:        349     329
 
Bme1580I G_kGCm'C

Cuts at:      0     110     678 
   Size:        110     568
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      45     593     678 
   Size:         45     548      85

  Fragments arranged by size: 

                 548      85      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     678 
   Size:         79     599
 
BsaHI Gr'CG_yC

Cuts at:      0      74      85     678 
   Size:         74      11     593

  Fragments arranged by size: 

                 593      74      11
 
BsaWI w'CCGG_w

Cuts at:      0     295     503     678 
   Size:        295     208     175

  Fragments arranged by size: 

                 295     208     175
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     211     678 
   Size:        211     467
 
BseYI C'CCAG_C

Cuts at:      0     109     678 
   Size:        109     569
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     678 
   Size:        297     381
 
BsiEI CG_ry'CG

Cuts at:      0      29     678 
   Size:         29     649
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     597     678 
   Size:        110     487      81

  Fragments arranged by size: 

                 487     110      81
 
BspEI T'CCGG_A

Cuts at:      0     503     678 
   Size:        503     175
 
BsrBI CCG'CTC

Cuts at:      0     267     471     678 
   Size:        267     204     207

  Fragments arranged by size: 

                 267     207     204
 
BsrFI r'CCGG_y

Cuts at:      0     120     289     295     678 
   Size:        120     169       6     383

  Fragments arranged by size: 

                 383     169     120       6
 
BsrGI T'GTAC_A

Cuts at:      0     659     678 
   Size:        659      19
 
BssHII G'CGCG_C

Cuts at:      0     368     370     678 
   Size:        368       2     308

  Fragments arranged by size: 

                 368     308       2
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     606     678 
   Size:        606      72
 
Bsu36I CC'TnA_GG

Cuts at:      0     275     678 
   Size:        275     403
 
BtgI C'CryG_G

Cuts at:      0      23      88     320     384     678 
   Size:         23      65     232      64     294

  Fragments arranged by size: 

                 294     232      65      64      23
 
ClaI AT'CG_AT

Cuts at:      0      44     678 
   Size:         44     634
 
DraIII CAC_nnn'GTG

Cuts at:      0     390     678 
   Size:        390     288
 
EaeI y'GGCC_r

Cuts at:      0       2      26     225     485     608     678 
   Size:          2      24     199     260     123      70

  Fragments arranged by size: 

                 260     199     123      70      24       2
 
EagI C'GGCC_G

Cuts at:      0      26     678 
   Size:         26     652
 
EarI CTCTTCn'nnn_

Cuts at:      0      62     176     678 
   Size:         62     114     502

  Fragments arranged by size: 

                 502     114      62
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     139     678 
   Size:        139     539
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     424     678 
   Size:         79     345     254

  Fragments arranged by size: 

                 345     254      79
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     649     678 
   Size:        649      29
 
HaeII r_GCGC'y

Cuts at:      0      88     312     420     502     678 
   Size:         88     224     108      82     176

  Fragments arranged by size: 

                 224     176     108      88      82
 
KasI G'GCGC_C

Cuts at:      0      84     678 
   Size:         84     594
 
MscI TGG'CCA

Cuts at:      0     610     678 
   Size:        610      68
 
MspA1I CmG'CkG

Cuts at:      0      25      90     240     322     678 
   Size:         25      65     150      82     356

  Fragments arranged by size: 

                 356     150      82      65      25
 
NaeI GCC'GGC

Cuts at:      0     122     291     678 
   Size:        122     169     387

  Fragments arranged by size: 

                 387     169     122
 
NarI GG'CG_CC

Cuts at:      0      85     678 
   Size:         85     593
 
NgoMIV G'CCGG_C

Cuts at:      0     120     289     678 
   Size:        120     169     389

  Fragments arranged by size: 

                 389     169     120
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     390     678 
   Size:        390     288
 
PfoI T'CCnGG_A

Cuts at:      0     541     678 
   Size:        541     137
 
PspOMI G'GGCC_C

Cuts at:      0     106     678 
   Size:        106     572
 
SacII CC_GC'GG

Cuts at:      0      26      91     323     678 
   Size:         26      65     232     355

  Fragments arranged by size: 

                 355     232      65      26
 
SfcI C'TryA_G

Cuts at:      0     645     678 
   Size:        645      33
 
SfoI GGC'GCC

Cuts at:      0      86     678 
   Size:         86     592
 
SgrAI Cr'CCGG_yG

Cuts at:      0     120     678 
   Size:        120     558
 
SmaI CCC'GGG

Cuts at:      0     105     216     678 
   Size:        105     111     462

  Fragments arranged by size: 

                 462     111     105
 
StuI AGG'CCT

Cuts at:      0     280     678 
   Size:        280     398
 
StyI C'CwwG_G

Cuts at:      0     633     678 
   Size:        633      45
 
TatI w'GTAC_w

Cuts at:      0     659     678 
   Size:        659      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     456     678 
   Size:        456     222
 
XmaI C'CCGG_G

Cuts at:      0     103     214     678 
   Size:        103     111     464

  Fragments arranged by size: 

                 464     111     103
 
XmnI GAAnn'nnTTC

Cuts at:      0     556     678 
   Size:        556     122
 
ZraI GAC'GTC

Cuts at:      0      75     678 
   Size:         75     603
 

 Enzymes that do cut:

    AatII     AcuI     AfeI     AgeI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI     BcgI     BglI     BlpI Bme1580I     BmrI     BpmI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI Bsp1286I    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BstXI   Bsu36I     BtgI     ClaI
   DraIII     EaeI     EagI     EarI     EciI  Eco57MI     FalI    HaeII
     KasI     MscI   MspA1I     NaeI     NarI   NgoMIV    PflMI     PfoI
   PspOMI    SacII     SfcI     SfoI    SgrAI     SmaI     StuI     StyI
     TatI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrDI
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     BtsI
    CspCI     DraI     DrdI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MslI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
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