Restriction map for pF1KB9681
 (Circular) (Six-base) MAPSORT of: pF1KB9681.seq  Check: 367  from: 1  to: 3533



pF1KB9681  3533 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:31  ..

AccI GT'mk_AC

Cuts at:    571     571 
   Size:       3533
 
Acc65I G'GTAC_C

Cuts at:    549     549 
   Size:       3533
 
AclI AA'CG_TT

Cuts at:   3373    3373 
   Size:       3533
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1358    1790    2537    2805    1358 
   Size:        432     747     268    2086

  Fragments arranged by size: 

                2086     747     432     268
 
AflIII A'CryG_T

Cuts at:    263     281     885    1989    2966     263 
   Size:         18     604    1104     977     830

  Fragments arranged by size: 

                1104     977     830     604      18
 
AgeI A'CCGG_T

Cuts at:   1921    1921 
   Size:       3533
 
AlwNI CAG_nnn'CTG

Cuts at:    838    2405     838 
   Size:       1567    1966
 
ApaLI G'TGCA_C

Cuts at:   2303    2303 
   Size:       3533
 
ApoI r'AATT_y

Cuts at:    430     537    2886    3142     430 
   Size:        107    2349     256     821

  Fragments arranged by size: 

                2349     821     256     107
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3533
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3533
 
AvaI C'yCGr_G

Cuts at:    553     553 
   Size:       3533
 
BamHI G'GATC_C

Cuts at:    558     558 
   Size:       3533
 
BanI G'GyrC_C

Cuts at:    549     963    1211    1246     549 
   Size:        414     248      35    2836

  Fragments arranged by size: 

                2836     414     248      35
 
BanII G_rGCy'C

Cuts at:    547    1577     547 
   Size:       1030    2503
 
BbeI G_GCGC'C

Cuts at:    967    1215     967 
   Size:        248    3285
 
BciVI GTATCCnnnnn_n'

Cuts at:   1431    2192    3533    1431 
   Size:        761    1341    1431

  Fragments arranged by size: 

                1431    1341     761
 
BclI T'GATC_A

Cuts at:   1053    1053 
   Size:       3533
 
BglII A'GATC_T

Cuts at:   1048    3085    1048 
   Size:       2037    1496
 
BlpI GC'TnA_GC

Cuts at:    645     645 
   Size:       3533
 
Bme1580I G_kGCm'C

Cuts at:   1158    1251    2307    1158 
   Size:         93    1056    2384

  Fragments arranged by size: 

                2384    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    745     826    1161    3305     745 
   Size:         81     335    2144     973

  Fragments arranged by size: 

                2144     973     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1900    1900 
   Size:       3533
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    709    1588    2080    2378     709 
   Size:        879     492     298    1864

  Fragments arranged by size: 

                1864     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3237    3237 
   Size:       3533
 
BsaAI yAC'GTr

Cuts at:    886     886 
   Size:       3533
 
BsaBI GATnn'nnATC

Cuts at:      6    1047    3084       6 
   Size:       1041    2037     455

  Fragments arranged by size: 

                2037    1041     455
 
BsaHI Gr'CG_yC

Cuts at:    964    1212    1914    3410     964 
   Size:        248     702    1496    1087

  Fragments arranged by size: 

                1496    1087     702     248
 
BsaWI w'CCGG_w

Cuts at:    722    1243    1921    2195    2342    2753     722 
   Size:        521     678     274     147     411    1502

  Fragments arranged by size: 

                1502     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    181     958    1716    2293    2789     181 
   Size:        777     758     577     496     925

  Fragments arranged by size: 

                 925     777     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1075    1121    2329     104 
   Size:        971      46    1208    1308

  Fragments arranged by size: 

                1308    1208     971      46
 
BsiHKAI G_wGCw'C

Cuts at:    547    1325    1515    2307     547 
   Size:        778     190     792    1773

  Fragments arranged by size: 

                1773     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    547    1158    1251    1325    1515    1577    2307     547 
   Size:        611      93      74     190      62     730    1773

  Fragments arranged by size: 

                1773     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2753    2753 
   Size:       3533
 
BspHI T'CATG_A

Cuts at:    366    2709     366 
   Size:       2343    1190
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    583     797    1099    1480     583 
   Size:        214     302     381    2636

  Fragments arranged by size: 

                2636     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1825    1879    3363    3528      50 
   Size:       1775      54    1484     165      55

  Fragments arranged by size: 

                1775    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1445    1445 
   Size:       3533
 
BsrFI r'CCGG_y

Cuts at:   1531    1712    1921    1531 
   Size:        181     209    3143

  Fragments arranged by size: 

                3143     209     181
 
BssSI C'ACGA_G

Cuts at:   1804    2162    1804 
   Size:        358    3175
 
BstAPI GCAn_nnn'nTGC

Cuts at:    910     910 
   Size:       3533
 
BstBI TT'CG_AA

Cuts at:   1894    1894 
   Size:       3533
 
BstEII G'GTnAC_C

Cuts at:    139     139 
   Size:       3533
 
BstYI r'GATC_y

Cuts at:      1     558    1025    1048    1383    1629    2641    2727    2739 
   Size:        557     467      23     335     246    1012      86      12

Cuts at:   2739    3085       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     557     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    338     338 
   Size:       3533
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1483    1664    1827    1483 
   Size:        181     163    3189

  Fragments arranged by size: 

                3189     181     163
 
BtsI GCAGTG_nn'

Cuts at:    776     776 
   Size:       3533
 
DraI TTT'AAA

Cuts at:    532     532 
   Size:       3533
 
DrdI GACnn_nn'nnGTC

Cuts at:   1239    2097    1239 
   Size:        858    2675
 
EaeI y'GGCC_r

Cuts at:   1118    1292    1683    1710    3055    1118 
   Size:        174     391      27    1345    1596

  Fragments arranged by size: 

                1596    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1118    1118 
   Size:       3533
 
EarI CTCTTCn'nnn_

Cuts at:   1556    1766    1556 
   Size:        210    3323
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1741    2051    2197    3343    1741 
   Size:        310     146    1146    1931

  Fragments arranged by size: 

                1931    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    545     545 
   Size:       3533
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1358    1790    2537    2805    1358 
   Size:        432     747     268    2086

  Fragments arranged by size: 

                2086     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    672     672 
   Size:       3533
 
EcoRI G'AATT_C

Cuts at:    537     537 
   Size:       3533
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    417    1552     417 
   Size:       1135    2398
 
FspI TGC'GCA

Cuts at:   1314    1314 
   Size:       3533
 
HaeII r_GCGC'y

Cuts at:    967    1215    2237    3216     967 
   Size:        248    1022     979    1284

  Fragments arranged by size: 

                1284    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1375    1405    1407    1437    1375 
   Size:         30       2      30    3471

  Fragments arranged by size: 

                3471      30      30       2
 
HincII GTy'rAC

Cuts at:    572     572 
   Size:       3533
 
HindIII A'AGCT_T

Cuts at:    117     117 
   Size:       3533
 
KasI G'GCGC_C

Cuts at:    963    1211     963 
   Size:        248    3285
 
KpnI G_GTAC'C

Cuts at:    553     553 
   Size:       3533
 
MluI A'CGCG_T

Cuts at:   2966    2966 
   Size:       3533
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2204    2388    2204 
   Size:        184    3349
 
MscI TGG'CCA

Cuts at:   1294    1294 
   Size:       3533
 
MslI CAynn'nnrTG

Cuts at:   1522    1649    1522 
   Size:        127    3406
 
MspA1I CmG'CkG

Cuts at:    181     340     644     958    1318    2331    2576     181 
   Size:        159     304     314     360    1013     245    1138

  Fragments arranged by size: 

                1138    1013     360     314     304     245     159
 
NaeI GCC'GGC

Cuts at:   1714    1714 
   Size:       3533
 
NarI GG'CG_CC

Cuts at:    964    1212     964 
   Size:        248    3285
 
NdeI CA'TA_TG

Cuts at:    347     347 
   Size:       3533
 
NgoMIV G'CCGG_C

Cuts at:   1712    1712 
   Size:       3533
 
NspI r_CATG'y

Cuts at:    267     285     586    1086    1993     267 
   Size:         18     301     500     907    1807

  Fragments arranged by size: 

                1807     907     500     301      18
 
PciI A'CATG_T

Cuts at:    263     281    1989     263 
   Size:         18    1708    1807

  Fragments arranged by size: 

                1807    1708      18
 
PmeI GTTT'AAAC

Cuts at:    532     532 
   Size:       3533
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2672    2704    2672 
   Size:         32    3501
 
PshAI GACnn'nnGTC

Cuts at:    209     209 
   Size:       3533
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    268     300     268 
   Size:         32    3501
 
PstI C_TGCA'G

Cuts at:    580    1265     580 
   Size:        685    2848
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3533
 
PvuII CAG'CTG

Cuts at:    181     958    1318     181 
   Size:        777     360    2396

  Fragments arranged by size: 

                2396     777     360
 
RsrII CG'GwC_CG

Cuts at:   1728    1728 
   Size:       3533
 
SacI G_AGCT'C

Cuts at:    547     547 
   Size:       3533
 
SacII CC_GC'GG

Cuts at:    341     341 
   Size:       3533
 
SalI G'TCGA_C

Cuts at:    570     570 
   Size:       3533
 
SapI GCTCTTCn'nnn_

Cuts at:   1556    1766    1556 
   Size:        210    3323
 
SbfI CC_TGCA'GG

Cuts at:    580     580 
   Size:       3533
 
ScaI AGT'ACT

Cuts at:   3050    3050 
   Size:       3533
 
SfcI C'TryA_G

Cuts at:     33     576    1261    2254    2445      33 
   Size:        543     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     543     191
 
SfoI GGC'GCC

Cuts at:    965    1213     965 
   Size:        248    3285
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3533
 
SmaI CCC'GGG

Cuts at:    555     555 
   Size:       3533
 
SmlI C'TyrA_G

Cuts at:    688    1603    2095    2357     688 
   Size:        915     492     262    1864

  Fragments arranged by size: 

                1864     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3533
 
SphI G_CATG'C

Cuts at:    586    1086     586 
   Size:        500    3033
 
StyI C'CwwG_G

Cuts at:    667    1021     667 
   Size:        354    3179
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1116    1249    1708    1917    1116 
   Size:        133     459     209    2732

  Fragments arranged by size: 

                2732     459     209     133
 
TatI w'GTAC_w

Cuts at:    284    3048     284 
   Size:       2764     769
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3050    3082    3050 
   Size:         32    3501
 
Tth111I GACn'n_nGTC

Cuts at:   1330    1330 
   Size:       3533
 
XbaI T'CTAG_A

Cuts at:    564     564 
   Size:       3533
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1028    1028 
   Size:       3533
 
XmaI C'CCGG_G

Cuts at:    553     553 
   Size:       3533
 
XmnI GAAnn'nnTTC

Cuts at:    223     223 
   Size:       3533
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NspI     PciI     PmeI     PpiI    PshAI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI
     MfeI     NcoI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI    PspXI    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     ZraI
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