Restriction map for pF1KB9675
##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MunI        MvnI        
NarI        NciI        NcoI        NdeI        NdeII       
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tru9I       TseI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Asp700I       209  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          79  GGTCC                G^G[AT]CC
BanII         153  GGGCTC               G[GA]GC[CT]^C
BmyI          153  GGGCTC               G[AGT]GC[ACT]^C
BpmI          175  CTGGAG               CTGGAG(16/14)
BsaAI          64  TACGTG               [CT]AC^GT[GA]
BseRI         218  CTCCTC               GAGGAG(10/8)
BsiYI         174  CCTGGAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          174  CCTGGAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          80  GTCCC                GGGAC(10/14)
Bsp1286I      153  GGGCTC               G[AGT]GC[ACT]^C
BsrFI          56  GCCGGC               [GA]^CCGG[CT]
BssKI         174  CCTGG                ^CC[ACGT]GG
BstAPI         90  GCAAGGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         174  CCTGG                CC^[AT]GG
Cac8I          56  GCCGGC               GC[ACGT]^[ACGT]GC
Cfr10I         56  GCCGGC               [GA]^CCGG[CT]
Cfr13I         79  GGTCC                G^G[ACGT]CC
DdeI           72  CTCAG                C^T[ACGT]AG
EaeI          128  CGGCCA               [CT]^GGCC[GA]
EcoNI         174  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        174  CCTGG                ^CC[AT]GG
HaeIII        129  GGCC                 GG^CC
HapII          57  CCGG                 C^CGG
HpaII          57  CCGG                 C^CGG
HphI          168  GGTGA                GGTGA(8/7)
MaeIII        105  GTCAC                ^GT[ACGT]AC
MspI           57  CCGG                 C^CGG
MvaI          174  CCTGG                CC^[AT]GG
NaeI           56  GCCGGC               GCC^GGC
NgoMIV         56  GCCGGC               G^CCGGC
NlaIII        203  CATG                 CATG^
NlaIV          79  GGTCCC               GG[ACGT]^[ACGT]CC
PleI           70  GACTC                GAGTC(4/5)
Sau96I         79  GGTCC                G^G[ACGT]CC
ScrFI         174  CCTGG                CC^[ACGT]GG
SmlI          226  CTCAAG               C^T[CT][GA]AG
TfiI           24  GATTC                G^A[AT]TC
Tsp45I        105  GTCAC                ^GT[GC]AC
Tsp509I       210  AATT                 ^AATT
XmnI          209  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           99  GCGG                 CCGC(-3/-1)
              127  GCGG                
AfaI           14  GTAC                 GT^AC
               63  GTAC                
EarI          221  CTCTTC               CTCTTC(1/4)
              118  GAAGAG              
Fnu4HI         99  GCGGC                GC^[ACGT]GC
              127  GCGGC               
HinfI          24  GATTC                G^A[ACGT]TC
               70  GACTC               
ItaI           99  GCGGC                GC^[ACGT]GC
              127  GCGGC               
Ksp632I       221  CTCTTC               CTCTTC(1/4)
              118  GAAGAG              
MaeII          65  ACGT                 A^CGT
              192  ACGT                
MboII         118  GAAGA                GAAGA(8/7)
              222  TCTTC               
MnlI           48  CCTC                 CCTC(7/6)
              220  CCTC                
MwoI           12  GCGTACCGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               90  GCAAGGACTGC         
RsaI           14  GTAC                 GT^AC
               63  GTAC                
TspRI          35  CAGTG                CA[GC]TG[ACGT][ACGT]^
              233  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################


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