Restriction map for pF1KB9673
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AvaI        AviII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvI        BcgI        BciVI       BcnI        
BfrI        BglI        BglII       BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        Hin1I       HincII      HindII      HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspI        MunI        MvaI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TseI        TthHB8I     
Van91I      XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI          164  AAATTT               [GA]^AATT[CT]
ApoI          164  AAATTT               [GA]^AATT[CT]
AspI          504  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         337  CCTAGG               C^CTAGG
BbvCI         617  CCTCAGC              CCTCAGC(-5/-2)
BlnI          337  CCTAGG               C^CTAGG
BpmI          587  CTCCAG               CTGGAG(16/14)
BseRI         254  CTCCTC               GAGGAG(10/8)
BsmAI         585  GTCTC                GTCTC(1/5)
BsrI          225  CCAGT                ACTGG(1/-1)
Cac8I          32  GCATGC               GC[ACGT]^[ACGT]GC
CfoI          233  GCGC                 GCG^C
DraII         333  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI          531  GACTCCCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          436  CCGCGG               C^C[GA][CT]GG
EcoO109I      333  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       337  CCTAGG               C^C[AT][AT]GG
EcoT22I       725  ATGCAT               ATGCA^T
Fnu4HI        230  GCCGC                GC^[ACGT]GC
HhaI          233  GCGC                 GCG^C
HinP1I        233  GCGC                 G^CGC
HindIII       599  AAGCTT               A^AGCTT
ItaI          230  GCCGC                GC^[ACGT]GC
KspI          436  CCGCGG               CCGC^GG
MaeII         741  ACGT                 A^CGT
MslI          681  CACTAATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV         658  GGGTCC               GG[ACGT]^[ACGT]CC
NsiI          725  ATGCAT               ATGCA^T
NspI           32  GCATGC               [GA]CATG^[CT]
PflFI         504  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         333  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         590  CAGCTG               CAG^CTG
SacII         436  CCGCGG               CCGC^GG
ScaI          472  AGTACT               AGT^ACT
SfcI          378  CTATAG               C^T[GA][CT]AG
SphI           32  GCATGC               GCATG^C
StyI          337  CCTAGG               C^C[AT][AT]GG
TfiI          715  GAATC                G^A[AT]TC
Tsp45I        139  GTGAC                ^GT[GC]AC
TspRI         226  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       504  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          150  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII         232  CGCG                 CG^CG
              437  CGCG                
AfaI          473  GTAC                 GT^AC
              565  GTAC                
AseI          640  ATTAAT               AT^TAAT
              651  ATTAAT              
AsnI          640  ATTAAT               AT^TAAT
              651  ATTAAT              
AvaII         334  GGTCC                G^G[AT]CC
              659  GGTCC               
BclI          237  TGATCA               T^GATCA
              317  TGATCA              
BsaJI         337  CCTAGG               C^C[ACGT][ACGT]GG
              436  CCGCGG              
BsiYI         325  CCATATTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              478  CCCACACTAGG         
BslI          325  CCATATTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              478  CCCACACTAGG         
BstUI         232  CGCG                 CG^CG
              437  CGCG                
Cfr13I        334  GGTCC                G^G[ACGT]CC
              659  GGTCC               
DpnII         238  GATC                 ^GATC
              318  GATC                
FauI          435  CCCGC                CCCGC(4/6)
              418  GCGGG               
FbaI          237  TGATCA               T^GATCA
              317  TGATCA              
MaeIII        139  GTGAC                ^GT[ACGT]AC
              457  GTTAC               
MboI          238  GATC                 ^GATC
              318  GATC                
MboII         422  GAAGA                GAAGA(8/7)
              745  GAAGA               
MspA1I        436  CCGCGG               C[AC]G^C[GT]G
              590  CAGCTG              
MvnI          232  CGCG                 CG^CG
              437  CGCG                
NdeII         238  GATC                 ^GATC
              318  GATC                
PleI          531  GACTC                GAGTC(4/5)
                4  GAGTC               
PshBI         640  ATTAAT               AT^TAAT
              651  ATTAAT              
RsaI          473  GTAC                 GT^AC
              565  GTAC                
Sau3AI        238  GATC                 ^GATC
              318  GATC                
Sau96I        334  GGTCC                G^G[ACGT]CC
              659  GGTCC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AciI        AluI        BfaI        BsmFI       DdeI        
HinfI       MaeI        MnlI        MseI        MwoI        
NlaIII      SfaNI       Tru9I       Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com