Restriction map for pF1KB9668
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AgeI        AhdI        AocI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvrII       BalI        BamHI       BciVI       
BclI        BfaI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaI        BsaWI       
BseAI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    EarI        Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     MaeI        MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        ScaI        
SexAI       SfiI        SfuI        SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        StyI        
SwaI        TfiI        Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcyI         1144  GGCGCC               G[GA]^CG[CT]C
AfaI          399  GTAC                 GT^AC
AflII        1371  CTTAAG               C^TTAAG
AflIII        401  ACACGT               A^C[GA][CT]GT
AlwI          798  GATCC                GGATC(4/5)
Aor51HI      1106  AGCGCT               AGC^GCT
BanI         1144  GGCGCC               G^G[CT][GA]CC
BbeI         1144  GGCGCC               GGCGC^C
BbrPI         402  CACGTG               CAC^GTG
BbsI           66  GAAGAC               GAAGAC(2/6)
BbvCI         444  CCTCAGC              CCTCAGC(-5/-2)
BfrI         1371  CTTAAG               C^TTAAG
BpuAI          66  GAAGAC               GAAGAC(2/6)
BsaAI         402  CACGTG               [CT]AC^GT[GA]
BsaHI        1144  GGCGCC               G[GA]^CG[CT]C
BseRI         304  GAGGAG               GAGGAG(10/8)
BsmAI         544  GAGAC                GTCTC(1/5)
BsmI         1252  GAATGC               GAATGC(1/-1)
BspMI          27  GCAGGT               ACCTGC(4/8)
BsrBI         979  GAGCGG               CCGCTC(-3/-3)
BsrDI          59  GCAATG               GCAATG(2/0)
BssSI          75  CACGAG               CACGAG(-5/-1)
BstYI         797  AGATCC               [GA]^GATC[CT]
DpnII         798  GATC                 ^GATC
Eco47III     1106  AGCGCT               AGC^GCT
EcoNI          84  CCTCTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          626  GACGC                GACGC(5/10)
Hin1I        1144  GGCGCC               G[GA]^CG[CT]C
HinfI           4  GAGTC                G^A[ACGT]TC
HphI          976  GGTGA                GGTGA(8/7)
KasI         1144  GGCGCC               G^GCGCC
MaeII         403  ACGT                 A^CGT
MboI          798  GATC                 ^GATC
MflI          797  AGATCC               [GA]^GATC[CT]
MseI         1372  TTAA                 T^TAA
NarI         1144  GGCGCC               GG^CGCC
NdeII         798  GATC                 ^GATC
NotI          729  GCGGCCGC             GC^GGCCGC
PleI            4  GAGTC                GAGTC(4/5)
PmaCI         402  CACGTG               CAC^GTG
PmlI          402  CACGTG               CAC^GTG
PpuMI          50  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         681  CAGCTG               CAG^CTG
RsaI          399  GTAC                 GT^AC
Sau3AI        798  GATC                 ^GATC
SbfI           24  CCTGCAGG             CCTGCA^GG
SfoI         1144  GGCGCC               GGC^GCC
SmlI         1371  CTTAAG               C^T[CT][GA]AG
Sse8387I       24  CCTGCAGG             CCTGCA^GG
TaqI          989  TCGA                 T^CGA
Tru9I        1372  TTAA                 T^TAA
Tsp509I       694  AATT                 ^AATT
TthHB8I       989  TCGA                 T^CGA
XhoII         797  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI         946  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1297  CAGCGCCTG           
ApaI          222  GGGCCC               GGGCC^C
              253  GGGCCC              
AvaI          409  CCCGAG               C^[CT]CG[GA]G
             1140  CTCGGG              
AvaII          20  GGACC                G^G[AT]CC
               51  GGTCC               
AviII        1246  TGCGCA               TGC^GCA
             1355  TGCGCA              
BsgI         1303  CTGCAC               GTGCAG(16/14)
              616  GTGCAG              
BsiEI         597  CGGCCG               CG[GA][CT]^CG
              730  CGGCCG              
BsoBI         409  CCCGAG               C^[CT]CG[GA]G
             1140  CTCGGG              
BssHII        515  GCGCGC               G^CGCGC
              725  GCGCGC              
BstAPI        491  GCAGGGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              945  GCAGCTCCTGC         
BstF5I        462  CATCC                GGATG(2/0)
              531  CATCC               
BstXI          10  CCAAGAGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              692  CCAATTTCCTGG        
EagI          597  CGGCCG               C^GGCCG
              730  CGGCCG              
EclXI         597  CGGCCG               C^GGCCG
              730  CGGCCG              
Eco52I        597  CGGCCG               C^GGCCG
              730  CGGCCG              
FokI          462  CATCC                GGATG(9/13)
              531  CATCC               
FspI         1246  TGCGCA               TGC^GCA
             1355  TGCGCA              
KspI         1185  CCGCGG               CCGC^GG
             1269  CCGCGG              
MboII          66  GAAGA                GAAGA(8/7)
              795  GAAGA               
MslI          609  CACGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              970  CACACTGGTG          
NlaIII       1020  CATG                 CATG^
             1072  CATG                
SacII        1185  CCGCGG               CCGC^GG
             1269  CCGCGG              
SfaNI         369  GCATC                GCATC(5/9)
              461  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AluI        Alw44I      ApaLI       
Asp700I     AspHI       BanII       BbvI        BcgI        
BcnI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BsrFI       
BsrI        BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIV       PstI        SacI        Sau96I      ScrFI       
SfcI        SgrAI       StuI        TseI        TspRI       
XmnI        
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