Restriction map for pF1KB9666
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BbeI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BsaBI       BsaHI       BsaWI       BseAI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    EarI        Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     MaeI        
MaeIII      MamI        MfeI        MluI        MroI        
MseI        MunI        NarI        NcoI        NdeI        
NheI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tsp45I      
Tsp509I     Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

Aor51HI       819  AGCGCT               AGC^GCT
AvaI          997  CTCGGG               C^[CT]CG[GA]G
BalI          140  TGGCCA               TGG^CCA
BamHI        1155  GGATCC               G^GATCC
BbrPI         838  CACGTG               CAC^GTG
BbsI            3  GTCTTC               GAAGAC(2/6)
BglI          313  GCCACCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI           3  GTCTTC               GAAGAC(2/6)
BsaAI         838  CACGTG               [CT]AC^GT[GA]
BsaI         1041  GGTCTC               GGTCTC(1/5)
BseRI         108  CTCCTC               GAGGAG(10/8)
BsmAI        1042  GTCTC                GTCTC(1/5)
BsmI          843  GCATTC               GAATGC(1/-1)
BsoBI         997  CTCGGG               C^[CT]CG[GA]G
BssSI        1358  CTCGTG               CACGAG(-5/-1)
BstAPI        937  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III      819  AGCGCT               AGC^GCT
HphI          908  TCACC                GGTGA(8/7)
MaeII         839  ACGT                 A^CGT
MluNI         140  TGGCCA               TGG^CCA
MscI          140  TGGCCA               TGG^CCA
MslI          928  CATGAGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          985  GCCGGC               GCC^GGC
NgoMIV        985  GCCGGC               G^CCGGC
PleI         1395  GAGTC                GAGTC(4/5)
PmaCI         838  CACGTG               CAC^GTG
PmlI          838  CACGTG               CAC^GTG
PpuMI        1053  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1240  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Asp700I       294  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              882  GAAGGCCTTC          
BanI          310  GGTGCC               G^G[CT][GA]CC
             1102  GGCACC              
BbvCI          75  CCTCAGC              CCTCAGC(-5/-2)
             1092  CCTCAGC             
BsgI          835  CTGCAC               GTGCAG(16/14)
              935  GTGCAG              
BsmFI         857  GTCCC                GGGAC(10/14)
             1247  GTCCC               
BsrBI         601  GAGCGG               CCGCTC(-3/-3)
              769  GAGCGG              
BsrFI         985  GCCGGC               [GA]^CCGG[CT]
             1387  ACCGGC              
BsrI          343  ACTGG                ACTGG(1/-1)
              360  CCAGT               
BstYI        1155  GGATCC               [GA]^GATC[CT]
             1186  AGATCC              
Cfr10I        985  GCCGGC               [GA]^CCGG[CT]
             1387  ACCGGC              
DraII        1053  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1431  GGGGCCT             
EcoO109I     1053  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1431  GGGGCCT             
EcoT14I       545  CCAAGG               C^C[AT][AT]GG
              797  CCAAGG              
HgaI         1240  GACGC                GACGC(5/10)
              855  GCGTC               
MboII           4  TCTTC                GAAGA(8/7)
             1216  TCTTC               
MflI         1155  GGATCC               [GA]^GATC[CT]
             1186  AGATCC              
NlaIII        928  CATG                 CATG^
             1023  CATG                
NotI          958  GCGGCCGC             GC^GGCCGC
              979  GCGGCCGC            
PflMI         132  CCAGGTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1225  CCAAAGGCTGG         
PvuII         950  CAGCTG               CAG^CTG
              968  CAGCTG              
StyI          545  CCAAGG               C^C[AT][AT]GG
              797  CCAAGG              
TfiI           51  GATTC                G^A[AT]TC
             1213  GAATC               
Van91I        132  CCAGGTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1225  CCAAAGGCTGG         
XhoII        1155  GGATCC               [GA]^GATC[CT]
             1186  AGATCC              
XmnI          294  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              882  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaII       AviII       BanII       
BbvI        BcgI        BcnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaJI       BsiEI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BspMI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EagI        EclXI       Eco52I      EcoRII      
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        KspI        MboI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIV       PstI        RsaI        
SacI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        TaqI        TseI        
TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com