Restriction map for pF1KB9666
 (Linear) (Six-base) MAPSORT of: KIBB9666.seq  Check: 7120  from: 1  to: 1443



KIBB9666.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     506     590     758     842    1443 
   Size:        506      84     168      84     601

  Fragments arranged by size: 

                 601     506     168      84      84
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285    1443 
   Size:        285    1158
 
AfeI AGC'GCT

Cuts at:      0     821    1443 
   Size:        821     622
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     950    1443 
   Size:        950     493
 
AlwNI CAG_nnn'CTG

Cuts at:      0     498     834     943    1297    1443 
   Size:        498     336     109     354     146

  Fragments arranged by size: 

                 498     354     336     146     109
 
AvaI C'yCGr_G

Cuts at:      0     997    1443 
   Size:        997     446
 
BamHI G'GATC_C

Cuts at:      0    1155    1443 
   Size:       1155     288
 
BanI G'GyrC_C

Cuts at:      0     310    1102    1443 
   Size:        310     792     341

  Fragments arranged by size: 

                 792     341     310
 
BanII G_rGCy'C

Cuts at:      0     565     649     733     817    1443 
   Size:        565      84      84      84     626

  Fragments arranged by size: 

                 626     565      84      84      84
 
BbvCI CC'TCA_GC

Cuts at:      0      76    1093    1443 
   Size:         76    1017     350

  Fragments arranged by size: 

                1017     350      76
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     366     400     450     484     618     652     702     736 
   Size:        366      34      50      34     134      34      50      34

Cuts at:    736    1443 
   Size:        707

  Fragments arranged by size: 

                 707     366     134      50      50      34      34      34
                  34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     319    1443 
   Size:        319    1124
 
BlpI GC'TnA_GC

Cuts at:      0     235     323     816    1443 
   Size:        235      88     493     627

  Fragments arranged by size: 

                 627     493     235      88
 
Bme1580I G_kGCm'C

Cuts at:      0     476     535    1147    1238    1262    1286    1310    1425 
   Size:        476      59     612      91      24      24      24     115

Cuts at:   1425    1443 
   Size:         18

  Fragments arranged by size: 

                 612     476     115      91      59      24      24      24
                  18
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     353    1443 
   Size:        353    1090
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811     885    1167    1443 
   Size:        811      74     282     276

  Fragments arranged by size: 

                 811     282     276      74
 
Bpu10I CC'TnA_GC

Cuts at:      0      76    1009    1093    1443 
   Size:         76     933      84     350

  Fragments arranged by size: 

                 933     350      84      76
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1047    1443 
   Size:       1047     396
 
BsaAI yAC'GTr

Cuts at:      0     840    1443 
   Size:        840     603
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121    1443 
   Size:         91      30    1322

  Fragments arranged by size: 

                1322      91      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      99    1443 
   Size:         99    1344
 
BseYI C'CCAG_C

Cuts at:      0    1159    1195    1231    1261    1285    1309    1374    1428 
   Size:       1159      36      36      30      24      24      65      54

Cuts at:   1428    1443 
   Size:         15

  Fragments arranged by size: 

                1159      65      54      36      36      30      24      24
                  15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820     956    1443 
   Size:        820     136     487

  Fragments arranged by size: 

                 820     487     136
 
BsiEI CG_ry'CG

Cuts at:      0     355     683     962     983    1443 
   Size:        355     328     279      21     460

  Fragments arranged by size: 

                 460     355     328     279      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     161     649     733     817    1076    1443 
   Size:        161     488      84      84     259     367

  Fragments arranged by size: 

                 488     367     259     161      84      84
 
BsmI GAATG_Cn'

Cuts at:      0     843    1443 
   Size:        843     600
 
Bsp1286I G_dGCh'C

Cuts at:      0     161     476     535     565     649     733     817    1076 
   Size:        161     315      59      30      84      84      84     259

Cuts at:   1076    1147    1238    1262    1286    1310    1425    1443 
   Size:         71      91      24      24      24     115      18

  Fragments arranged by size: 

                 315     259     161     115      91      84      84      84
                  71      59      30      24      24      24      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     506     590     758     842    1029    1443 
   Size:        506      84     168      84     187     414

  Fragments arranged by size: 

                 506     414     187     168      84      84
 
BsrBI CCG'CTC

Cuts at:      0     603     771    1443 
   Size:        603     168     672

  Fragments arranged by size: 

                 672     603     168
 
BsrFI r'CCGG_y

Cuts at:      0     985    1387    1443 
   Size:        985     402      56

  Fragments arranged by size: 

                 985     402      56
 
BssSI C'ACGA_G

Cuts at:      0    1358    1443 
   Size:       1358      85
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     943    1443 
   Size:        943     500
 
BstYI r'GATC_y

Cuts at:      0    1155    1186    1443 
   Size:       1155      31     257

  Fragments arranged by size: 

                1155     257      31
 
BtgI C'CryG_G

Cuts at:      0     152     306     330     517     956    1443 
   Size:        152     154      24     187     439     487

  Fragments arranged by size: 

                 487     439     187     154     152      24
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     229     397    1443 
   Size:        229     168    1046

  Fragments arranged by size: 

                1046     229     168
 
BtsI GCAGTG_nn'

Cuts at:      0     119     444     628     705     789    1423    1443 
   Size:        119     325     184      77      84     634      20

  Fragments arranged by size: 

                 634     325     184     119      84      77      20
 
EaeI y'GGCC_r

Cuts at:      0     140     352     555     680     807     891     959     980 
   Size:        140     212     203     125     127      84      68      21

Cuts at:    980    1113    1443 
   Size:        133     330

  Fragments arranged by size: 

                 330     212     203     140     133     127     125      84
                  68      21
 
EagI C'GGCC_G

Cuts at:      0     352     680     959     980    1443 
   Size:        352     328     279      21     463

  Fragments arranged by size: 

                 463     352     328     279      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     208    1443 
   Size:        208    1235
 
EcoICRI GAG'CTC

Cuts at:      0     647     731     815    1443 
   Size:        647      84      84     628

  Fragments arranged by size: 

                 647     628      84      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285     811     885    1167    1443 
   Size:        285     526      74     282     276

  Fragments arranged by size: 

                 526     285     282     276      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1054    1432    1443 
   Size:       1054     378      11

  Fragments arranged by size: 

                1054     378      11
 
FspI TGC'GCA

Cuts at:      0     502     586     754    1443 
   Size:        502      84     168     689

  Fragments arranged by size: 

                 689     502     168      84
 
HaeII r_GCGC'y

Cuts at:      0     396     655     690     823    1443 
   Size:        396     259      35     133     620

  Fragments arranged by size: 

                 620     396     259     133      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      42      74      91     123     228     260    1117    1147 
   Size:         42      32      17      32     105      32     857      30

Cuts at:   1147    1149    1179    1443 
   Size:          2      30     264

  Fragments arranged by size: 

                 857     264     105      42      32      32      32      30
                  30      17       2
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     758    1443 
   Size:        758     685
 
MscI TGG'CCA

Cuts at:      0     142    1443 
   Size:        142    1301
 
MslI CAynn'nnrTG

Cuts at:      0     932    1443 
   Size:        932     511
 
MspA1I CmG'CkG

Cuts at:      0     154     260     278     428     519     698     943     952 
   Size:        154     106      18     150      91     179     245       9

Cuts at:    952     958     964     970     979    1443 
   Size:          6       6       6       9     464

  Fragments arranged by size: 

                 464     245     179     154     150     106      91      18
                   9       9       6       6       6
 
NaeI GCC'GGC

Cuts at:      0     987    1443 
   Size:        987     456
 
NgoMIV G'CCGG_C

Cuts at:      0     985    1443 
   Size:        985     458
 
NotI GC'GGCC_GC

Cuts at:      0     959     980    1443 
   Size:        959      21     463

  Fragments arranged by size: 

                 959     463      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     138    1231    1443 
   Size:        138    1093     212

  Fragments arranged by size: 

                1093     212     138
 
PmlI CAC'GTG

Cuts at:      0     840    1443 
   Size:        840     603
 
PpuMI rG'GwC_Cy

Cuts at:      0    1054    1443 
   Size:       1054     389
 
PshAI GACnn'nnGTC

Cuts at:      0    1244    1443 
   Size:       1244     199
 
PstI C_TGCA'G

Cuts at:      0     662     951     969     975    1443 
   Size:        662     289      18       6     468

  Fragments arranged by size: 

                 662     468     289      18       6
 
PvuII CAG'CTG

Cuts at:      0     952     970    1443 
   Size:        952      18     473

  Fragments arranged by size: 

                 952     473      18
 
SacI G_AGCT'C

Cuts at:      0     649     733     817    1443 
   Size:        649      84      84     626

  Fragments arranged by size: 

                 649     626      84      84
 
SacII CC_GC'GG

Cuts at:      0     155     520     959    1443 
   Size:        155     365     439     484

  Fragments arranged by size: 

                 484     439     365     155
 
SfcI C'TryA_G

Cuts at:      0     658     947     965     971    1443 
   Size:        658     289      18       6     472

  Fragments arranged by size: 

                 658     472     289      18       6
 
StuI AGG'CCT

Cuts at:      0     382     466     634     802     886     898    1443 
   Size:        382      84     168     168      84      12     545

  Fragments arranged by size: 

                 545     382     168     168      84      84      12
 
StyI C'CwwG_G

Cuts at:      0     545     797    1443 
   Size:        545     252     646

  Fragments arranged by size: 

                 646     545     252
 
XmnI GAAnn'nnTTC

Cuts at:      0     298     886    1443 
   Size:        298     588     557

  Fragments arranged by size: 

                 588     557     298
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AlfI    AlwNI     AvaI    BamHI     BanI
    BanII    BbvCI     BcgI     BglI     BlpI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstAPI
    BstYI     BtgI    BtgZI     BtsI     EaeI     EagI     EciI  EcoICRI
  Eco57MI EcoO109I     FspI    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NaeI   NgoMIV     NotI    PflMI     PmlI    PpuMI    PshAI
     PstI    PvuII     SacI    SacII     SfcI     StuI     StyI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BbsI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmtI     BplI    BpuEI    BsaBI    BsaHI    BsaWI    BsiWI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
     EarI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NcoI
     NdeI     NheI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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