Restriction map for pF1KB9653
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BciVI       BclI        BfaI        BfrI        BlnI        
BpmI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsmAI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        Eam1105I    
EarI        Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
HphI        KpnI        Ksp632I     MaeI        MaeIII      
MamI        MfeI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       Van91I      XbaI        
XcmI        XhoI        


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# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccI          188  GTCTAC               GT^[AC][GT]AC
AluI          791  AGCT                 AG^CT
AlwI          705  GATCC                GGATC(4/5)
Aor51HI       119  AGCGCT               AGC^GCT
Asp700I        36  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           16  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          442  GTCTTC               GAAGAC(2/6)
BglI          296  GCCTGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         542  AGATCT               A^GATCT
BlpI           61  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       61  GCTCAGC              GC^T[ACGT]AGC
BpuAI         442  GTCTTC               GAAGAC(2/6)
BseRI         256  CTCCTC               GAGGAG(10/8)
BsgI          349  CTGCAC               GTGCAG(16/14)
BsiWI         569  CGTACG               C^GTACG
BsrBI         751  CCGCTC               CCGCTC(-3/-3)
BstF5I        762  CATCC                GGATG(2/0)
BstXI           5  CCAAGCCGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         542  AGATCT               [GA]^GATC[CT]
CelII          61  GCTCAGC              GC^T[ACGT]AGC
Eco47III      119  AGCGCT               AGC^GCT
EcoNI         258  CCTCAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          762  CATCC                GGATG(9/13)
MaeII          20  ACGT                 A^CGT
MflI          542  AGATCT               [GA]^GATC[CT]
MslI          198  CATGCACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI          16  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         790  CAGCTG               CAG^CTG
SfaNI          96  GATGC                GCATC(5/9)
Tth111I        16  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         542  AGATCT               [GA]^GATC[CT]
XmnI           36  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          228  GTAC                 GT^AC
              570  GTAC                
AflIII        438  ACGCGT               A^C[GA][CT]GT
              572  ACGCGT              
AspHI         211  GAGCAC               G[AT]GC[AT]^C
              319  GAGCAC              
AvaII          15  GGACC                G^G[AT]CC
              795  GGACC               
BanII          59  GGGCTC               G[GA]GC[CT]^C
              525  GGGCTC              
BcgI          318  CGAGCACCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              538  GCAGAGATCTCG        
BsiHKAI       211  GAGCAC               G[AT]GC[AT]^C
              319  GAGCAC              
BsrFI           9  GCCGGT               [GA]^CCGG[CT]
              230  ACCGGC              
Cfr10I          9  GCCGGT               [GA]^CCGG[CT]
              230  ACCGGC              
DdeI           62  CTCAG                C^T[ACGT]AG
              380  CTGAG               
HinfI         151  GAGTC                G^A[ACGT]TC
              382  GAGTC               
MboII         443  TCTTC                GAAGA(8/7)
              446  TCTTC               
MluI          438  ACGCGT               A^CGCGT
              572  ACGCGT              
PleI          151  GAGTC                GAGTC(4/5)
              382  GAGTC               
RsaI          228  GTAC                 GT^AC
              570  GTAC                
SmlI          259  CTCAAG               C^T[CT][GA]AG
              331  CTCAAG              
TaqI          173  TCGA                 T^CGA
              518  TCGA                
TthHB8I       173  TCGA                 T^CGA
              518  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AvaI        BanI        
BbeI        BbvI        BcnI        BmyI        BsaHI       
BsaJI       BsiEI       BsiYI       BslI        BsoBI       
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DpnII       DsaI        EaeI        
EagI        EclXI       Eco52I      EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaII       ItaI        
KasI        KspI        MboI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NdeII       NlaIII      NlaIV       NotI        
PstI        SacII       Sau3AI      Sau96I      ScrFI       
SfcI        SfoI        SmaI        TseI        XmaCI       
XmaI        
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