Restriction map for pF1KB9653
 (Linear) (Six-base) MAPSORT of: KIBB9653.seq  Check: 6488  from: 1  to: 828



KIBB9653.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AccI GT'mk_AC

Cuts at:      0     189     828 
   Size:        189     639
 
AfeI AGC'GCT

Cuts at:      0     121     828 
   Size:        121     707
 
AflIII A'CryG_T

Cuts at:      0     438     572     828 
   Size:        438     134     256

  Fragments arranged by size: 

                 438     256     134
 
AvaI C'yCGr_G

Cuts at:      0     400     684     693     736     828 
   Size:        400     284       9      43      92

  Fragments arranged by size: 

                 400     284      92      43       9
 
BanI G'GyrC_C

Cuts at:      0     127     285     340     375     607     828 
   Size:        127     158      55      35     232     221

  Fragments arranged by size: 

                 232     221     158     127      55      35
 
BanII G_rGCy'C

Cuts at:      0      63     529     828 
   Size:         63     466     299

  Fragments arranged by size: 

                 466     299      63
 
BbeI G_GCGC'C

Cuts at:      0     131     344     611     828 
   Size:        131     213     267     217

  Fragments arranged by size: 

                 267     217     213     131
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     435     828 
   Size:        435     393
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     307     341     527     561     828 
   Size:        307      34     186      34     267

  Fragments arranged by size: 

                 307     267     186      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     302     828 
   Size:        302     526
 
BglII A'GATC_T

Cuts at:      0     542     828 
   Size:        542     286
 
BlpI GC'TnA_GC

Cuts at:      0      62     828 
   Size:         62     766
 
Bme1580I G_kGCm'C

Cuts at:      0     290     665     828 
   Size:        290     375     163

  Fragments arranged by size: 

                 375     290     163
 
BmgBI CAC'GTC

Cuts at:      0      21     828 
   Size:         21     807
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     316     828 
   Size:        244      72     512

  Fragments arranged by size: 

                 512     244      72
 
BsaHI Gr'CG_yC

Cuts at:      0     128     314     341     608     828 
   Size:        128     186      27     267     220

  Fragments arranged by size: 

                 267     220     186     128      27
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     511     541     828 
   Size:        511      30     287

  Fragments arranged by size: 

                 511     287      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     247     828 
   Size:        247     581
 
BseYI C'CCAG_C

Cuts at:      0     582     679     730     788     828 
   Size:        582      97      51      58      40

  Fragments arranged by size: 

                 582      97      58      51      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     828 
   Size:        334     494
 
BsiEI CG_ry'CG

Cuts at:      0     166     235     413     419     461     596     647     828 
   Size:        166      69     178       6      42     135      51     181

  Fragments arranged by size: 

                 181     178     166     135      69      51      42       6
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     215     323     828 
   Size:        215     108     505

  Fragments arranged by size: 

                 505     215     108
 
BsiWI C'GTAC_G

Cuts at:      0     569     828 
   Size:        569     259
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     215     290     323     529     665     828 
   Size:         63     152      75      33     206     136     163

  Fragments arranged by size: 

                 206     163     152     136      75      63      33
 
BsrBI CCG'CTC

Cuts at:      0     753     828 
   Size:        753      75
 
BsrFI r'CCGG_y

Cuts at:      0       9     230     828 
   Size:          9     221     598

  Fragments arranged by size: 

                 598     221       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12     828 
   Size:         12     816
 
BstYI r'GATC_y

Cuts at:      0     542     828 
   Size:        542     286
 
BtgI C'CryG_G

Cuts at:      0     235     413     494     596     615     806     828 
   Size:        235     178      81     102      19     191      22

  Fragments arranged by size: 

                 235     191     178     102      81      22      19
 
EaeI y'GGCC_r

Cuts at:      0     163     232     410     416     458     644     828 
   Size:        163      69     178       6      42     186     184

  Fragments arranged by size: 

                 186     184     178     163      69      42       6
 
EagI C'GGCC_G

Cuts at:      0     163     232     410     416     458     644     828 
   Size:        163      69     178       6      42     186     184

  Fragments arranged by size: 

                 186     184     178     163      69      42       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     325     828 
   Size:        325     503
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     262     828 
   Size:        262     566
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     294     828 
   Size:        294     534
 
HaeII r_GCGC'y

Cuts at:      0     123     131     344     611     828 
   Size:        123       8     213     267     217

  Fragments arranged by size: 

                 267     217     213     123       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     511     532     543     564     828 
   Size:        511      21      11      21     264

  Fragments arranged by size: 

                 511     264      21      21      11
 
KasI G'GCGC_C

Cuts at:      0     127     340     607     828 
   Size:        127     213     267     221

  Fragments arranged by size: 

                 267     221     213     127
 
MluI A'CGCG_T

Cuts at:      0     438     572     828 
   Size:        438     134     256

  Fragments arranged by size: 

                 438     256     134
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     828 
   Size:        134     694
 
MslI CAynn'nnrTG

Cuts at:      0     202     828 
   Size:        202     626
 
MspA1I CmG'CkG

Cuts at:      0      66     237     415     427     463     475     496     598 
   Size:         66     171     178      12      36      12      21     102

Cuts at:    598     640     792     808     828 
   Size:         42     152      16      20

  Fragments arranged by size: 

                 178     171     152     102      66      42      36      21
                  20      16      12      12
 
NarI GG'CG_CC

Cuts at:      0     128     341     608     828 
   Size:        128     213     267     220

  Fragments arranged by size: 

                 267     220     213     128
 
NotI GC'GGCC_GC

Cuts at:      0     410     416     644     828 
   Size:        410       6     228     184

  Fragments arranged by size: 

                 410     228     184       6
 
PstI C_TGCA'G

Cuts at:      0     639     721     746     828 
   Size:        639      82      25      82

  Fragments arranged by size: 

                 639      82      82      25
 
PvuII CAG'CTG

Cuts at:      0     792     828 
   Size:        792      36
 
SacII CC_GC'GG

Cuts at:      0     238     416     497     599     809     828 
   Size:        238     178      81     102     210      19

  Fragments arranged by size: 

                 238     210     178     102      81      19
 
SfcI C'TryA_G

Cuts at:      0     635     717     742     828 
   Size:        635      82      25      86

  Fragments arranged by size: 

                 635      86      82      25
 
SfoI GGC'GCC

Cuts at:      0     129     342     609     828 
   Size:        129     213     267     219

  Fragments arranged by size: 

                 267     219     213     129
 
SmaI CCC'GGG

Cuts at:      0     686     695     738     828 
   Size:        686       9      43      90

  Fragments arranged by size: 

                 686      90      43       9
 
SmlI C'TyrA_G

Cuts at:      0     259     331     828 
   Size:        259      72     497

  Fragments arranged by size: 

                 497     259      72
 
SrfI GCCC'GGGC

Cuts at:      0     686     828 
   Size:        686     142
 
Tth111I GACn'n_nGTC

Cuts at:      0      19     828 
   Size:         19     809
 
XmaI C'CCGG_G

Cuts at:      0     684     693     736     828 
   Size:        684       9      43      92

  Fragments arranged by size: 

                 684      92      43       9
 
XmnI GAAnn'nnTTC

Cuts at:      0      40     828 
   Size:         40     788
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     AvaI     BanI    BanII     BbeI     BbsI
     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI    BpuEI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BsrBI    BsrFI    BstXI    BstYI     BtgI     EaeI     EagI     EciI
    EcoNI     FalI    HaeII    Hin4I     KasI     MluI     MmeI     MslI
   MspA1I     NarI     NotI     PstI    PvuII    SacII     SfcI     SfoI
     SmaI     SmlI     SrfI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI
    BfrBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MscI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     ZraI
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