Restriction map for pF1KB9637
 (Linear) (Six-base) MAPSORT of: KIBB9637.seq  Check: 2495  from: 1  to: 1233



KIBB9637.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565    1233 
   Size:        565     668
 
AleI CACnn'nnGTG

Cuts at:      0      32     241    1233 
   Size:         32     209     992

  Fragments arranged by size: 

                 992     209      32
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     259    1233 
   Size:        259     974
 
AlwNI CAG_nnn'CTG

Cuts at:      0     474     675     949    1233 
   Size:        474     201     274     284

  Fragments arranged by size: 

                 474     284     274     201
 
BanI G'GyrC_C

Cuts at:      0     167    1233 
   Size:        167    1066
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      55     463    1233 
   Size:         55     408     770

  Fragments arranged by size: 

                 770     408      55
 
BbvCI CC'TCA_GC

Cuts at:      0     617    1233 
   Size:        617     616
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     720    1233 
   Size:        720     513
 
BfrBI ATG'CAT

Cuts at:      0    1225    1233 
   Size:       1225       8
 
Bme1580I G_kGCm'C

Cuts at:      0     222     414    1135    1233 
   Size:        222     192     721      98

  Fragments arranged by size: 

                 721     222     192      98
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     244    1233 
   Size:         70     174     989

  Fragments arranged by size: 

                 989     174      70
 
Bpu10I CC'TnA_GC

Cuts at:      0     617     899    1233 
   Size:        617     282     334

  Fragments arranged by size: 

                 617     334     282
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     494    1233 
   Size:        494     739
 
BsaHI Gr'CG_yC

Cuts at:      0     360    1233 
   Size:        360     873
 
BsaWI w'CCGG_w

Cuts at:      0      59     496    1233 
   Size:         59     437     737

  Fragments arranged by size: 

                 737     437      59
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      27    1233 
   Size:         27    1206
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139     713    1233 
   Size:        139     574     520

  Fragments arranged by size: 

                 574     520     139
 
BseYI C'CCAG_C

Cuts at:      0     153     659    1233 
   Size:        153     506     574

  Fragments arranged by size: 

                 574     506     153
 
BsiHKAI G_wGCw'C

Cuts at:      0      39     238     514    1233 
   Size:         39     199     276     719

  Fragments arranged by size: 

                 719     276     199      39
 
BsmI GAATG_Cn'

Cuts at:      0    1225    1233 
   Size:       1225       8
 
Bsp1286I G_dGCh'C

Cuts at:      0      39     222     238     414     514    1135    1233 
   Size:         39     183      16     176     100     621      98

  Fragments arranged by size: 

                 621     183     176     100      98      39      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     932     975    1233 
   Size:        932      43     258

  Fragments arranged by size: 

                 932     258      43
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     675    1233 
   Size:        675     558
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     108     421    1233 
   Size:        108     313     812

  Fragments arranged by size: 

                 812     313     108
 
BtgI C'CryG_G

Cuts at:      0    1127    1233 
   Size:       1127     106
 
BtsI GCAGTG_nn'

Cuts at:      0     198    1233 
   Size:        198    1035
 
DraIII CAC_nnn'GTG

Cuts at:      0     369    1233 
   Size:        369     864
 
EaeI y'GGCC_r

Cuts at:      0     185    1233 
   Size:        185    1048
 
EarI CTCTTCn'nnn_

Cuts at:      0     269    1233 
   Size:        269     964
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     716    1233 
   Size:        716     517
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     244     565    1233 
   Size:         70     174     321     668

  Fragments arranged by size: 

                 668     321     174      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     928    1233 
   Size:        928     305
 
EcoO109I rG'GnC_Cy

Cuts at:      0     198    1060    1233 
   Size:        198     862     173

  Fragments arranged by size: 

                 862     198     173
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     847     879    1233 
   Size:        847      32     354

  Fragments arranged by size: 

                 847     354      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     564    1233 
   Size:        564     669
 
MslI CAynn'nnrTG

Cuts at:      0      32     241    1233 
   Size:         32     209     992

  Fragments arranged by size: 

                 992     209      32
 
MspA1I CmG'CkG

Cuts at:      0     616     675    1008    1186    1233 
   Size:        616      59     333     178      47

  Fragments arranged by size: 

                 616     333     178      59      47
 
NcoI C'CATG_G

Cuts at:      0    1127    1233 
   Size:       1127     106
 
NsiI A_TGCA'T

Cuts at:      0    1227    1233 
   Size:       1227       6
 
PasI CC'CwG_GG

Cuts at:      0    1056    1233 
   Size:       1056     177
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     369    1233 
   Size:        369     864
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      37      69    1233 
   Size:         37      32    1164

  Fragments arranged by size: 

                1164      37      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     198    1233 
   Size:        198    1035
 
PstI C_TGCA'G

Cuts at:      0     945    1233 
   Size:        945     288
 
PvuII CAG'CTG

Cuts at:      0     616    1008    1186    1233 
   Size:        616     392     178      47

  Fragments arranged by size: 

                 616     392     178      47
 
SexAI A'CCwGG_T

Cuts at:      0     365    1233 
   Size:        365     868
 
SfcI C'TryA_G

Cuts at:      0     941    1233 
   Size:        941     292
 
StyI C'CwwG_G

Cuts at:      0     296     929    1127    1233 
   Size:        296     633     198     106

  Fragments arranged by size: 

                 633     296     198     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     107    1233 
   Size:        107    1126
 
TatI w'GTAC_w

Cuts at:      0     140     749    1233 
   Size:        140     609     484

  Fragments arranged by size: 

                 609     484     140
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     382     414     624     656    1233 
   Size:        382      32     210      32     577

  Fragments arranged by size: 

                 577     382     210      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1033    1233 
   Size:       1033     200
 
XmnI GAAnn'nnTTC

Cuts at:      0      52    1233 
   Size:         52    1181
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     BanI     BbsI    BbvCI    BciVI
    BfrBI Bme1580I     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI   BstAPI    BstXI
     BtgI     BtsI   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    Hin4I     MmeI     MslI   MspA1I     NcoI     NsiI     PasI
    PflMI     PpiI    PpuMI     PstI    PvuII    SexAI     SfcI     StyI
    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI
     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaBI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PciI     PfoI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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