Restriction map for pF1KB9634
 (Linear) (Six-base) MAPSORT of: KIBB9634.seq  Check: 7268  from: 1  to: 975



KIBB9634.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

Acc65I G'GTAC_C

Cuts at:      0     876     975 
   Size:        876      99
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     558     640     975 
   Size:        141     417      82     335

  Fragments arranged by size: 

                 417     335     141      82
 
AflIII A'CryG_T

Cuts at:      0     847     975 
   Size:        847     128
 
AhdI GACnn_n'nnGTC

Cuts at:      0     947     975 
   Size:        947      28
 
AlwNI CAG_nnn'CTG

Cuts at:      0     404     975 
   Size:        404     571
 
ApaI G_GGCC'C

Cuts at:      0     548     777     975 
   Size:        548     229     198

  Fragments arranged by size: 

                 548     229     198
 
AvaI C'yCGr_G

Cuts at:      0     118     726     975 
   Size:        118     608     249

  Fragments arranged by size: 

                 608     249     118
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     731     764     975 
   Size:        731      33     211

  Fragments arranged by size: 

                 731     211      33
 
BanI G'GyrC_C

Cuts at:      0     699     876     975 
   Size:        699     177      99

  Fragments arranged by size: 

                 699     177      99
 
BanII G_rGCy'C

Cuts at:      0     548     616     727     777     975 
   Size:        548      68     111      50     198

  Fragments arranged by size: 

                 548     198     111      68      50
 
BbvCI CC'TCA_GC

Cuts at:      0     237     273     975 
   Size:        237      36     702

  Fragments arranged by size: 

                 702     237      36
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      19     725     732     975 
   Size:         19     706       7     243

  Fragments arranged by size: 

                 706     243      19       7
 
Bme1580I G_kGCm'C

Cuts at:      0     548     777     975 
   Size:        548     229     198

  Fragments arranged by size: 

                 548     229     198
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     349     975 
   Size:        349     626
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360     542     975 
   Size:        360     182     433

  Fragments arranged by size: 

                 433     360     182
 
Bpu10I CC'TnA_GC

Cuts at:      0     237     273     346     975 
   Size:        237      36      73     629

  Fragments arranged by size: 

                 629     237      73      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739     975 
   Size:        739     236
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     955     975 
   Size:        955      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     225     234     237     246     956     975 
   Size:        225       9       3       9     710      19

  Fragments arranged by size: 

                 710     225      19       9       9       3
 
BseYI C'CCAG_C

Cuts at:      0     151     430     446     487     592     598     651     975 
   Size:        151     279      16      41     105       6      53     324

  Fragments arranged by size: 

                 324     279     151     105      53      41      16       6
            
 
Bsp1286I G_dGCh'C

Cuts at:      0     548     616     727     777     975 
   Size:        548      68     111      50     198

  Fragments arranged by size: 

                 548     198     111      68      50
 
BsrBI CCG'CTC

Cuts at:      0     350     770     975 
   Size:        350     420     205

  Fragments arranged by size: 

                 420     350     205
 
BsrFI r'CCGG_y

Cuts at:      0     586     975 
   Size:        586     389
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     404     975 
   Size:        404     571
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     108     835     975 
   Size:        108     727     140

  Fragments arranged by size: 

                 727     140     108
 
BtgI C'CryG_G

Cuts at:      0     101     975 
   Size:        101     874
 
BtsI GCAGTG_nn'

Cuts at:      0     140     975 
   Size:        140     835
 
EaeI y'GGCC_r

Cuts at:      0     110     474     975 
   Size:        110     364     501

  Fragments arranged by size: 

                 501     364     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     360     542     558     640     975 
   Size:        141     219     182      16      82     335

  Fragments arranged by size: 

                 335     219     182     141      82      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0      46     366     545     773     774     897     941     975 
   Size:         46     320     179     228       1     123      44      34

  Fragments arranged by size: 

                 320     228     179     123      46      44      34       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     885     975 
   Size:        885      90
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387     933     965     975 
   Size:        355      32     546      32      10

  Fragments arranged by size: 

                 546     355      32      32      10
 
KpnI G_GTAC'C

Cuts at:      0     880     975 
   Size:        880      95
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     887     975 
   Size:        887      88
 
MscI TGG'CCA

Cuts at:      0     112     975 
   Size:        112     863
 
MslI CAynn'nnrTG

Cuts at:      0     166     836     975 
   Size:        166     670     139

  Fragments arranged by size: 

                 670     166     139
 
MspA1I CmG'CkG

Cuts at:      0     133     210     528     651     797     975 
   Size:        133      77     318     123     146     178

  Fragments arranged by size: 

                 318     178     146     133     123      77
 
NcoI C'CATG_G

Cuts at:      0     101     975 
   Size:        101     874
 
NspI r_CATG'y

Cuts at:      0     851     975 
   Size:        851     124
 
PasI CC'CwG_GG

Cuts at:      0     458     957     975 
   Size:        458     499      18

  Fragments arranged by size: 

                 499     458      18
 
PciI A'CATG_T

Cuts at:      0     847     975 
   Size:        847     128
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     651     975 
   Size:        651     324
 
PfoI T'CCnGG_A

Cuts at:      0      42     975 
   Size:         42     933
 
PpuMI rG'GwC_Cy

Cuts at:      0      46     941     975 
   Size:         46     895      34

  Fragments arranged by size: 

                 895      46      34
 
PspOMI G'GGCC_C

Cuts at:      0     544     773     975 
   Size:        544     229     202

  Fragments arranged by size: 

                 544     229     202
 
PvuII CAG'CTG

Cuts at:      0     210     528     651     975 
   Size:        210     318     123     324

  Fragments arranged by size: 

                 324     318     210     123
 
SanDI GG'GwC_CC

Cuts at:      0      46     975 
   Size:         46     929
 
SmlI C'TyrA_G

Cuts at:      0     718     975 
   Size:        718     257
 
StuI AGG'CCT

Cuts at:      0     506     975 
   Size:        506     469
 
StyI C'CwwG_G

Cuts at:      0     101     758     975 
   Size:        101     657     217

  Fragments arranged by size: 

                 657     217     101
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     658     717     975 
   Size:        658      59     258

  Fragments arranged by size: 

                 658     258      59
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      19     105     600     648     975 
   Size:         19      86     495      48     327

  Fragments arranged by size: 

                 495     327      86      48      19
 
XmnI GAAnn'nnTTC

Cuts at:      0     625     975 
   Size:        625     350
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AhdI    AlwNI     ApaI     AvaI     BaeI
     BanI    BanII    BbvCI    BciVI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BseRI    BseYI Bsp1286I    BsrBI    BsrFI   BstAPI
    BstXI     BtgI     BtsI     EaeI  Eco57MI EcoO109I     FalI    Hin4I
     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PasI
     PciI    PflMI     PfoI    PpuMI   PspOMI    PvuII    SanDI     SmlI
     StuI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
    BamHI     BbeI     BbsI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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