Restriction map for pF1KB9627
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
AocI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvrII       
BalI        BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BpmI        BsaBI       BsaI        BsaWI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FokI        FseI        HincII      
HindII      HpaI        KpnI        KspI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp45I      Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AhdI          550  GACCTAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          525  GATCC                GGATC(4/5)
Aor51HI       350  AGCGCT               AGC^GCT
AspEI         550  GACCTAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         797  GGACC                G^G[AT]CC
AviII         431  TGCGCA               TGC^GCA
BbeI          658  GGCGCC               GGCGC^C
BlpI          894  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      894  GCTCAGC              GC^T[ACGT]AGC
BsaAI         562  TACGTG               [CT]AC^GT[GA]
BsgI          565  GTGCAG               GTGCAG(16/14)
BspMI         428  ACCTGC               ACCTGC(4/8)
BssHII        340  GCGCGC               G^CGCGC
BssSI         955  CACGAG               CACGAG(-5/-1)
BstAPI        786  GCACGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         524  AGATCC               [GA]^GATC[CT]
CelII         894  GCTCAGC              GC^T[ACGT]AGC
DdeI          895  CTCAG                C^T[ACGT]AG
Eam1105I      550  GACCTAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      350  AGCGCT               AGC^GCT
EcoNI         429  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       335  CCAAGG               C^C[AT][AT]GG
FspI          431  TGCGCA               TGC^GCA
HindIII       358  AAGCTT               A^AGCTT
HphI         1095  TCACC                GGTGA(8/7)
KasI          658  GGCGCC               G^GCGCC
MflI          524  AGATCC               [GA]^GATC[CT]
MspA1I         57  CAGCTG               C[AC]G^C[GT]G
NarI          658  GGCGCC               GG^CGCC
NspI          398  GCATGC               [GA]CATG^[CT]
PstI          622  CTGCAG               CTGCA^G
PvuII          57  CAGCTG               CAG^CTG
SacI         1123  GAGCTC               GAGCT^C
SfcI          622  CTGCAG               C^T[GA][CT]AG
SfiI          201  GGCCACGTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          658  GGCGCC               GGC^GCC
SmlI          931  CTCAAG               C^T[CT][GA]AG
SphI          398  GCATGC               GCATG^C
StyI          335  CCAAGG               C^C[AT][AT]GG
TaqI          479  TCGA                 T^CGA
TfiI         1068  GAATC                G^A[AT]TC
TspRI         748  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       479  TCGA                 T^CGA
XhoII         524  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI          658  GGCGCC               G[GA]^CG[CT]C
             1054  GGCGTC              
AlwNI         463  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              568  CAGACTCTG           
AspHI         726  GTGCTC               G[AT]GC[AT]^C
             1123  GAGCTC              
BbsI           73  GAAGAC               GAAGAC(2/6)
             1027  GTCTTC              
BpuAI          73  GAAGAC               GAAGAC(2/6)
             1027  GTCTTC              
BsaHI         658  GGCGCC               G[GA]^CG[CT]C
             1054  GGCGTC              
BsiHKAI       726  GTGCTC               G[AT]GC[AT]^C
             1123  GAGCTC              
BsmBI         481  GAGACG               CGTCTC(1/5)
              823  GAGACG              
BsmFI         193  GGGAC                GGGAC(10/14)
              172  GTCCC               
BsrI          276  ACTGG                ACTGG(1/-1)
              169  CCAGT               
BstXI         599  CCACCAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              602  CCAATCTGGTGG        
DpnII         477  GATC                 ^GATC
              525  GATC                
EagI          672  CGGCCG               C^GGCCG
              758  CGGCCG              
EarI          187  GAAGAG               CTCTTC(1/4)
              256  GAAGAG              
EclXI         672  CGGCCG               C^GGCCG
              758  CGGCCG              
Eco52I        672  CGGCCG               C^GGCCG
              758  CGGCCG              
Hin1I         658  GGCGCC               G[GA]^CG[CT]C
             1054  GGCGTC              
Ksp632I       187  GAAGAG               CTCTTC(1/4)
              256  GAAGAG              
MboI          477  GATC                 ^GATC
              525  GATC                
NdeII         477  GATC                 ^GATC
              525  GATC                
PleI          570  GACTC                GAGTC(4/5)
              940  GACTC               
Sau3AI        477  GATC                 ^GATC
              525  GATC                
SmaI          130  CCCGGG               CCC^GGG
              155  CCCGGG              
XmaCI         130  CCCGGG               C^CCGGG
              155  CCCGGG              
XmaI          130  CCCGGG               C^CCGGG
              155  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        ApaI        
AvaI        BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BsaJI       BseRI       
BsiEI       BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
ItaI        MaeI        MaeII       MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NgoMIV      NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       TseI        XcmI        
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