Restriction map for pF1KB9616
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        MaeI        
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MscI        MseI        MslI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    RsaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       Van91I      XbaI        
XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII        276  TCCGGA               T^CCGGA
AspI           33  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         405  GGTCC                G^G[AT]CC
AviII         119  TGCGCA               TGC^GCA
BglI          655  GCCGTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          316  GAGACC               GGTCTC(1/5)
BseAI         276  TCCGGA               T^CCGGA
BspEI         276  TCCGGA               T^CCGGA
BspHI          29  TCATGA               T^CATGA
BsrFI         183  GCCGGC               [GA]^CCGG[CT]
BsrI          402  ACTGG                ACTGG(1/-1)
BssSI         646  CTCGTG               CACGAG(-5/-1)
BstF5I        458  CATCC                GGATG(2/0)
BstNI         519  CCTGG                CC^[AT]GG
Cfr10I        183  GCCGGC               [GA]^CCGG[CT]
DdeI          379  CTGAG                C^T[ACGT]AG
DpnII         472  GATC                 ^GATC
DraII         158  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EarI          442  GAAGAG               CTCTTC(1/4)
EcoO109I      158  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        519  CCTGG                ^CC[AT]GG
EcoT14I       149  CCAAGG               C^C[AT][AT]GG
FokI          458  CATCC                GGATG(9/13)
FspI          119  TGCGCA               TGC^GCA
HphI          262  GGTGA                GGTGA(8/7)
Ksp632I       442  GAAGAG               CTCTTC(1/4)
KspI          569  CCGCGG               CCGC^GG
MaeII         641  ACGT                 A^CGT
MboI          472  GATC                 ^GATC
MroI          276  TCCGGA               T^CCGGA
MvaI          519  CCTGG                CC^[AT]GG
NaeI          183  GCCGGC               GCC^GGC
NdeII         472  GATC                 ^GATC
NgoMIV        183  GCCGGC               G^CCGGC
PaeR7I        163  CTCGAG               C^TCGAG
PflFI          33  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI          471  CGATCG               CGAT^CG
PvuII         103  CAGCTG               CAG^CTG
RcaI           29  TCATGA               T^CATGA
SacI          133  GAGCTC               GAGCT^C
SacII         569  CCGCGG               CCGC^GG
Sau3AI        472  GATC                 ^GATC
SfaNI          26  GCATC                GCATC(5/9)
SfiI          654  GGCCGTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          128  CCCGGG               CCC^GGG
SmlI          163  CTCGAG               C^T[CT][GA]AG
StyI          149  CCAAGG               C^C[AT][AT]GG
TaqI          164  TCGA                 T^CGA
Tth111I        33  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       164  TCGA                 T^CGA
XhoI          163  CTCGAG               C^TCGAG
XmaCI         128  CCCGGG               C^CCGGG
XmaI          128  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          104  AGCT                 AG^CT
              134  AGCT                
BanII         133  GAGCTC               G[GA]GC[CT]^C
              166  GAGCCC              
BsaWI          92  ACCGGA               [AT]^CCGG[AT]
              276  TCCGGA              
BseRI         313  GAGGAG               GAGGAG(10/8)
              445  GAGGAG              
BsmAI         244  GAGAC                GTCTC(1/5)
              316  GAGAC               
BsmFI         392  GGGAC                GGGAC(10/14)
              406  GTCCC               
EagI          185  CGGCCG               C^GGCCG
              239  CGGCCG              
EclXI         185  CGGCCG               C^GGCCG
              239  CGGCCG              
Eco52I        185  CGGCCG               C^GGCCG
              239  CGGCCG              
HinfI          96  GAGTC                G^A[ACGT]TC
              232  GAGTC               
MspA1I        103  CAGCTG               C[AC]G^C[GT]G
              569  CCGCGG              
NspI           65  GCATGC               [GA]CATG^[CT]
              632  GCATGC              
PleI           96  GAGTC                GAGTC(4/5)
              232  GAGTC               
SphI           65  GCATGC               GCATG^C
              632  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AccII       AciI        AcyI        AspHI       AvaI        
BanI        BbeI        BbvI        BcnI        BmyI        
BsaHI       BsaJI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsoBI       Bsp1286I    BssKI       
BstUI       Cac8I       CfoI        Cfr13I      DsaI        
EaeI        FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       ItaI        KasI        MboII       MnlI        
MspI        MvnI        MwoI        NarI        NciI        
NlaIII      NlaIV       PstI        Sau96I      ScrFI       
SfcI        SfoI        TseI        
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