Restriction map for pF1KB9616
 (Linear) (Six-base) MAPSORT of: KIBB9616.seq  Check: 1767  from: 1  to: 669



KIBB9616.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AvaI C'yCGr_G

Cuts at:      0     128     163     169     221     408     669 
   Size:        128      35       6      52     187     261

  Fragments arranged by size: 

                 261     187     128      52      35       6
 
BanI G'GyrC_C

Cuts at:      0     300     505     585     669 
   Size:        300     205      80      84

  Fragments arranged by size: 

                 300     205      84      80
 
BanII G_rGCy'C

Cuts at:      0     137     170     669 
   Size:        137      33     499

  Fragments arranged by size: 

                 499     137      33
 
BbeI G_GCGC'C

Cuts at:      0     304     509     589     669 
   Size:        304     205      80      80

  Fragments arranged by size: 

                 304     205      80      80
 
BglI GCCn_nnn'nGGC

Cuts at:      0     661     669 
   Size:        661       8
 
Bme1580I G_kGCm'C

Cuts at:      0     336     669 
   Size:        336     333
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     336     669 
   Size:        336     333
 
Bpu10I CC'TnA_GC

Cuts at:      0     379     669 
   Size:        379     290
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     310     669 
   Size:        310     359
 
BsaHI Gr'CG_yC

Cuts at:      0     301     506     586     669 
   Size:        301     205      80      83

  Fragments arranged by size: 

                 301     205      83      80
 
BsaWI w'CCGG_w

Cuts at:      0      92     276     669 
   Size:         92     184     393

  Fragments arranged by size: 

                 393     184      92
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     439     469     669 
   Size:        439      30     200

  Fragments arranged by size: 

                 439     200      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     328     460     669 
   Size:        328     132     209

  Fragments arranged by size: 

                 328     209     132
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28      73     196     669 
   Size:         28      45     123     473

  Fragments arranged by size: 

                 473     123      45      28
 
BsiEI CG_ry'CG

Cuts at:      0     188     242     474     669 
   Size:        188      54     232     195

  Fragments arranged by size: 

                 232     195     188      54
 
BsiHKAI G_wGCw'C

Cuts at:      0     137     269     647     669 
   Size:        137     132     378      22

  Fragments arranged by size: 

                 378     137     132      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     170     269     336     647     669 
   Size:        137      33      99      67     311      22

  Fragments arranged by size: 

                 311     137      99      67      33      22
 
BspEI T'CCGG_A

Cuts at:      0     276     669 
   Size:        276     393
 
BspHI T'CATG_A

Cuts at:      0      29     669 
   Size:         29     640
 
BsrFI r'CCGG_y

Cuts at:      0     183     669 
   Size:        183     486
 
BssSI C'ACGA_G

Cuts at:      0     646     669 
   Size:        646      23
 
BtgI C'CryG_G

Cuts at:      0     236     569     656     669 
   Size:        236     333      87      13

  Fragments arranged by size: 

                 333     236      87      13
 
EaeI y'GGCC_r

Cuts at:      0     146     185     239     669 
   Size:        146      39      54     430

  Fragments arranged by size: 

                 430     146      54      39
 
EagI C'GGCC_G

Cuts at:      0     185     239     669 
   Size:        185      54     430

  Fragments arranged by size: 

                 430     185      54
 
EarI CTCTTCn'nnn_

Cuts at:      0     437     669 
   Size:        437     232
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     312     640     669 
   Size:        312     328      29

  Fragments arranged by size: 

                 328     312      29
 
EcoICRI GAG'CTC

Cuts at:      0     135     669 
   Size:        135     534
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159     669 
   Size:        159     510
 
FspI TGC'GCA

Cuts at:      0     121     669 
   Size:        121     548
 
HaeII r_GCGC'y

Cuts at:      0     128     304     509     543     589     669 
   Size:        128     176     205      34      46      80

  Fragments arranged by size: 

                 205     176     128      80      46      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471     669 
   Size:        439      32     198

  Fragments arranged by size: 

                 439     198      32
 
KasI G'GCGC_C

Cuts at:      0     300     505     585     669 
   Size:        300     205      80      84

  Fragments arranged by size: 

                 300     205      84      80
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     669 
   Size:        604      65
 
MspA1I CmG'CkG

Cuts at:      0     105     571     669 
   Size:        105     466      98

  Fragments arranged by size: 

                 466     105      98
 
NaeI GCC'GGC

Cuts at:      0     185     669 
   Size:        185     484
 
NarI GG'CG_CC

Cuts at:      0     301     506     586     669 
   Size:        301     205      80      83

  Fragments arranged by size: 

                 301     205      83      80
 
NgoMIV G'CCGG_C

Cuts at:      0     183     669 
   Size:        183     486
 
NspI r_CATG'y

Cuts at:      0      69     636     669 
   Size:         69     567      33

  Fragments arranged by size: 

                 567      69      33
 
PspXI vC'TCGA_Gb

Cuts at:      0     163     669 
   Size:        163     506
 
PstI C_TGCA'G

Cuts at:      0     104     482     536     565     669 
   Size:        104     378      54      29     104

  Fragments arranged by size: 

                 378     104     104      54      29
 
PvuI CG_AT'CG

Cuts at:      0     474     669 
   Size:        474     195
 
PvuII CAG'CTG

Cuts at:      0     105     669 
   Size:        105     564
 
SacI G_AGCT'C

Cuts at:      0     137     669 
   Size:        137     532
 
SacII CC_GC'GG

Cuts at:      0     572     669 
   Size:        572      97
 
SfcI C'TryA_G

Cuts at:      0     100     478     532     561     669 
   Size:        100     378      54      29     108

  Fragments arranged by size: 

                 378     108     100      54      29
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     661     669 
   Size:        661       8
 
SfoI GGC'GCC

Cuts at:      0     302     507     587     669 
   Size:        302     205      80      82

  Fragments arranged by size: 

                 302     205      82      80
 
SmaI CCC'GGG

Cuts at:      0     130     669 
   Size:        130     539
 
SmlI C'TyrA_G

Cuts at:      0     163     669 
   Size:        163     506
 
SphI G_CATG'C

Cuts at:      0      69     636     669 
   Size:         69     567      33

  Fragments arranged by size: 

                 567      69      33
 
StyI C'CwwG_G

Cuts at:      0     149     669 
   Size:        149     520
 
Tth111I GACn'n_nGTC

Cuts at:      0      36     669 
   Size:         36     633
 
XhoI C'TCGA_G

Cuts at:      0     163     669 
   Size:        163     506
 
XmaI C'CCGG_G

Cuts at:      0     128     669 
   Size:        128     541
 

 Enzymes that do cut:

     AvaI     BanI    BanII     BbeI     BglI Bme1580I     BplI   Bpu10I
     BsaI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BsrFI    BssSI     BtgI     EaeI     EagI
     EarI     EciI  EcoICRI EcoO109I     FspI    HaeII    Hin4I     KasI
     MmeI   MspA1I     NaeI     NarI   NgoMIV     NspI    PspXI     PstI
     PvuI    PvuII     SacI    SacII     SfcI     SfiI     SfoI     SmaI
     SmlI     SphI     StyI  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI     BpmI    BpuEI    BsaAI    BsaBI    BseYI
    BsiWI     BsmI    BsmBI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XmnI     ZraI
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