Restriction map for
pF1KB9609
(Circular) (Six-base) MAPSORT of: pF1KB9609.seq Check: 5705 from: 1 to: 3836
pF1KB9609 3836 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 504 588 1661 2093 2840 3108 168
Size: 336 84 1073 432 747 268 896
Fragments arranged by size:
1073 896 747 432 336 268 84
AflIII A'CryG_T
Cuts at: 1188 2292 3269 1188
Size: 1104 977 1755
Fragments arranged by size:
1755 1104 977
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AhdI GACnn_n'nnGTC
Cuts at: 437 437
Size: 3836
AlwNI CAG_nnn'CTG
Cuts at: 1141 2708 1141
Size: 1567 2269
ApaLI G'TGCA_C
Cuts at: 355 2606 355
Size: 2251 1585
ApoI r'AATT_y
Cuts at: 218 386 470 722 820 840 3189 3445 218
Size: 168 84 252 98 20 2349 256 609
Fragments arranged by size:
2349 609 256 252 168 98 84 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 856 856
Size: 3836
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 852 1266 1514 1549 852
Size: 414 248 35 3139
Fragments arranged by size:
3139 414 248 35
BanII G_rGCy'C
Cuts at: 850 1880 850
Size: 1030 2806
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BbsI GAAGACnn'nnnn_
Cuts at: 356 356
Size: 3836
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 282 359 1461 1554 2610 282
Size: 77 1102 93 1056 1508
Fragments arranged by size:
1508 1102 1056 93 77
BmrI ACTGGGnnnn_n'
Cuts at: 1048 1129 1464 3608 1048
Size: 81 335 2144 1276
Fragments arranged by size:
2144 1276 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 416 332
Size: 84 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1891 2383 2681 1012
Size: 879 492 298 2167
Fragments arranged by size:
2167 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3540 3540
Size: 3836
BsaAI yAC'GTr
Cuts at: 1189 1189
Size: 3836
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 1267 1515 2217 3713 1267
Size: 248 702 1496 1390
Fragments arranged by size:
1496 1390 702 248
BsaWI w'CCGG_w
Cuts at: 1025 1546 2224 2498 2645 3056 1025
Size: 521 678 274 147 411 1805
Fragments arranged by size:
1805 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 281 1261 2019 2596 3092 281
Size: 980 758 577 496 1025
Fragments arranged by size:
1025 980 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 771 445
Size: 326 3510
BsiEI CG_ry'CG
Cuts at: 104 1378 1424 2632 104
Size: 1274 46 1208 1308
Fragments arranged by size:
1308 1274 1208 46
BsiHKAI G_wGCw'C
Cuts at: 359 850 1628 1818 2610 359
Size: 491 778 190 792 1585
Fragments arranged by size:
1585 792 778 491 190
BsmI GAATG_Cn'
Cuts at: 166 502 166
Size: 336 3500
Bsp1286I G_dGCh'C
Cuts at: 282 359 850 1461 1554 1628 1818 1880 2610
Size: 77 491 611 93 74 190 62 730
Cuts at: 2610 282
Size: 1508
Fragments arranged by size:
1508 730 611 491 190 93 77 74
62
BspEI T'CCGG_A
Cuts at: 3056 3056
Size: 3836
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 886 1100 1402 1783 886
Size: 214 302 381 2939
Fragments arranged by size:
2939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1748 1748
Size: 3836
BsrFI r'CCGG_y
Cuts at: 1834 2015 2224 1834
Size: 181 209 3446
Fragments arranged by size:
3446 209 181
BssSI C'ACGA_G
Cuts at: 2107 2465 2107
Size: 358 3478
BstAPI GCAn_nnn'nTGC
Cuts at: 758 1213 758
Size: 455 3381
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstXI CCAn_nnnn'nTGG
Cuts at: 676 676
Size: 3836
BstYI r'GATC_y
Cuts at: 1 861 1328 1351 1686 1932 2944 3030 3042
Size: 860 467 23 335 246 1012 86 12
Cuts at: 3042 3388 1
Size: 346 449
Fragments arranged by size:
1012 860 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 171 1079 171
Size: 908 2928
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 489 524 573 608 489
Size: 35 49 35 3717
Fragments arranged by size:
3717 49 35 35
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DraIII CAC_nnn'GTG
Cuts at: 562 646 562
Size: 84 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1542 2400 1542
Size: 858 2978
EaeI y'GGCC_r
Cuts at: 1421 1595 1986 2013 3358 1421
Size: 174 391 27 1345 1899
Fragments arranged by size:
1899 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 255 621 1859 2069 255
Size: 366 1238 210 2022
Fragments arranged by size:
2022 1238 366 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2044 2354 2500 3646 2044
Size: 310 146 1146 2234
Fragments arranged by size:
2234 1146 310 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 332 416 504 588 1661 2093 2840 3108
Size: 164 84 88 84 1073 432 747 268
Cuts at: 3108 168
Size: 896
Fragments arranged by size:
1073 896 747 432 268 164 88 84
84
EcoO109I rG'GnC_Cy
Cuts at: 975 975
Size: 3836
EcoRI G'AATT_C
Cuts at: 218 386 470 722 840 218
Size: 168 84 252 118 3214
Fragments arranged by size:
3214 252 168 118 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 611 1617 611
Size: 1006 2830
FspAI rTGC'GCAy
Cuts at: 611 611
Size: 3836
HaeII r_GCGC'y
Cuts at: 527 1270 1518 2540 3519 527
Size: 743 248 1022 979 844
Fragments arranged by size:
1022 979 844 743 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 423 455 1678 1708 1710 1740 423
Size: 32 1223 30 2 30 2519
Fragments arranged by size:
2519 1223 32 30 30 2
HincII GTy'rAC
Cuts at: 712 875 712
Size: 163 3673
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2691 2507
Size: 184 3652
MscI TGG'CCA
Cuts at: 1597 1597
Size: 3836
MslI CAynn'nnrTG
Cuts at: 411 479 674 731 1825 1952 411
Size: 68 195 57 1094 127 2295
Fragments arranged by size:
2295 1094 195 127 68 57
MspA1I CmG'CkG
Cuts at: 947 1261 1621 2634 2879 947
Size: 314 360 1013 245 1904
Fragments arranged by size:
1904 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 3836
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 3836
NspI r_CATG'y
Cuts at: 668 889 1389 2296 668
Size: 221 500 907 2208
Fragments arranged by size:
2208 907 500 221
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PstI C_TGCA'G
Cuts at: 781 883 1568 781
Size: 102 685 3049
Fragments arranged by size:
3049 685 102
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 1261 1621 1261
Size: 360 3476
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 255 1859 2069 255
Size: 1604 210 2022
Fragments arranged by size:
2022 1604 210
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SfcI C'TryA_G
Cuts at: 33 777 801 879 1564 2557 2748 33
Size: 744 24 78 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 191 78 24
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 991 1906 2398 2660 991
Size: 915 492 262 2167
Fragments arranged by size:
2167 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StuI AGG'CCT
Cuts at: 181 685 181
Size: 504 3332
StyI C'CwwG_G
Cuts at: 970 1324 970
Size: 354 3482
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1419 1552 2011 2220 1419
Size: 133 459 209 3035
Fragments arranged by size:
3035 459 209 133
TatI w'GTAC_w
Cuts at: 3351 3351
Size: 3836
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 349 381 3353 3385 349
Size: 32 2972 32 800
Fragments arranged by size:
2972 800 32 32
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI EcoNI EcoRV FseI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI