Restriction map for pF1KB9607
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BsrDI       BsrFI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EaeI        
EagI        EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AfaI          291  GTAC                 GT^AC
AflIII        293  ACATGT               A^C[GA][CT]GT
AhdI          202  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        496  GTGCAC               G^TGCAC
AlwI          118  GGATC                GGATC(4/5)
ApaI           63  GGGCCC               GGGCC^C
ApaLI         496  GTGCAC               G^TGCAC
Asp700I       363  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         202  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         100  GGACC                G^G[AT]CC
BanI          395  GGCACC               G^G[CT][GA]CC
BbsI          283  GTCTTC               GAAGAC(2/6)
BbvCI          25  CCTCAGC              CCTCAGC(-5/-2)
BglI          229  GCCAGTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          239  CTCCAG               CTGGAG(16/14)
BpuAI         283  GTCTTC               GAAGAC(2/6)
BsaI           52  GAGACC               GGTCTC(1/5)
BsmAI          52  GAGAC                GTCTC(1/5)
BsmFI         181  GTCCC                GGGAC(10/14)
BsmI          367  GCATTC               GAATGC(1/-1)
BspLU11I      293  ACATGT               A^CATGT
BspMI         158  ACCTGC               ACCTGC(4/8)
BsrI          230  CCAGT                ACTGG(1/-1)
BssHII        375  GCGCGC               G^CGCGC
BstYI         118  GGATCT               [GA]^GATC[CT]
DpnII         119  GATC                 ^GATC
DraIII        573  CACGCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      202  GACGAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HinfI         208  GAGTC                G^A[ACGT]TC
MboI          119  GATC                 ^GATC
MboII         284  TCTTC                GAAGA(8/7)
MflI          118  GGATCT               [GA]^GATC[CT]
MspA1I        261  CAGCTG               C[AC]G^C[GT]G
NcoI           56  CCATGG               C^CATGG
NdeII         119  GATC                 ^GATC
NspI          293  ACATGT               [GA]CATG^[CT]
PflMI          91  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          208  GAGTC                GAGTC(4/5)
PstI          562  CTGCAG               CTGCA^G
PvuII         261  CAGCTG               CAG^CTG
RsaI          291  GTAC                 GT^AC
Sau3AI        119  GATC                 ^GATC
SmlI          276  CTCAAG               C^T[CT][GA]AG
Van91I         91  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         118  GGATCT               [GA]^GATC[CT]
XmnI          363  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII         376  CGCG                 CG^CG
              575  CGCG                
AluI          262  AGCT                 AG^CT
              476  AGCT                
AlwNI          28  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
               92  CAGAACCTG           
AspHI         273  GTGCTC               G[AT]GC[AT]^C
              496  GTGCAC              
BanII          63  GGGCCC               G[GA]GC[CT]^C
              236  GGGCTC              
BcnI          462  CCGGG                CC^[GC]GG
              492  CCGGG               
BsiHKAI       273  GTGCTC               G[AT]GC[AT]^C
              496  GTGCAC              
BsrBI          84  CCGCTC               CCGCTC(-3/-3)
              442  CCGCTC              
BstUI         376  CGCG                 CG^CG
              575  CGCG                
BstXI         380  CCAGCCACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              404  CCATTCACCTGG        
CfoI          375  GCGC                 GCG^C
              377  GCGC                
DdeI           26  CTCAG                C^T[ACGT]AG
              173  CTCAG               
DraII          62  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               63  GGGCCCT             
DsaI           56  CCATGG               C^C[GA][CT]GG
              432  CCACGG              
EcoO109I       62  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               63  GGGCCCT             
EcoT14I        56  CCATGG               C^C[AT][AT]GG
              142  CCAAGG              
HapII         462  CCGG                 C^CGG
              492  CCGG                
HhaI          375  GCGC                 GCG^C
              377  GCGC                
HinP1I        375  GCGC                 G^CGC
              377  GCGC                
HpaII         462  CCGG                 C^CGG
              492  CCGG                
MaeIII        210  GTCAC                ^GT[ACGT]AC
              350  GTGAC               
MspI          462  CCGG                 C^CGG
              492  CCGG                
MvnI          376  CGCG                 CG^CG
              575  CGCG                
NciI          462  CCGGG                CC^[GC]GG
              492  CCGGG               
NlaIII         57  CATG                 CATG^
              294  CATG                
SfcI          258  CTACAG               C^T[GA][CT]AG
              562  CTGCAG              
StyI           56  CCATGG               C^C[AT][AT]GG
              142  CCAAGG              
TaqI          287  TCGA                 T^CGA
              344  TCGA                
Tsp45I        210  GTCAC                ^GT[GC]AC
              350  GTGAC               
TthHB8I       287  TCGA                 T^CGA
              344  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        BbvI        BmyI        BsaJI       BseRI       
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HphI        ItaI        
MnlI        MvaI        MwoI        NlaIV       Sau96I      
ScrFI       SfaNI       TseI        TspRI       
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