Restriction map for pF1KB9607
 (Linear) (Six-base) MAPSORT of: KIBB9607.seq  Check: 4154  from: 1  to: 591



KIBB9607.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     591 
   Size:        289     302
 
AflIII A'CryG_T

Cuts at:      0     293     591 
   Size:        293     298
 
AhdI GACnn_n'nnGTC

Cuts at:      0     207     591 
   Size:        207     384
 
AlwNI CAG_nnn'CTG

Cuts at:      0      33      97     591 
   Size:         33      64     494

  Fragments arranged by size: 

                 494      64      33
 
ApaI G_GGCC'C

Cuts at:      0      67     591 
   Size:         67     524
 
ApaLI G'TGCA_C

Cuts at:      0     496     591 
   Size:        496      95
 
BanI G'GyrC_C

Cuts at:      0     395     591 
   Size:        395     196
 
BanII G_rGCy'C

Cuts at:      0      67     240     591 
   Size:         67     173     351

  Fragments arranged by size: 

                 351     173      67
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     276     591 
   Size:        276     315
 
BbvCI CC'TCA_GC

Cuts at:      0      26     591 
   Size:         26     565
 
BglI GCCn_nnn'nGGC

Cuts at:      0     235     591 
   Size:        235     356
 
Bme1580I G_kGCm'C

Cuts at:      0      67     500     591 
   Size:         67     433      91

  Fragments arranged by size: 

                 433      91      67
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     591 
   Size:        224     367
 
Bpu10I CC'TnA_GC

Cuts at:      0      26     591 
   Size:         26     565
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261     591 
   Size:        261     330
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      46     591 
   Size:         46     545
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      99     128     220     591 
   Size:         99      29      92     371

  Fragments arranged by size: 

                 371      99      92      29
 
BseYI C'CCAG_C

Cuts at:      0     482     591 
   Size:        482     109
 
BsiHKAI G_wGCw'C

Cuts at:      0     277     500     591 
   Size:        277     223      91

  Fragments arranged by size: 

                 277     223      91
 
BsmI GAATG_Cn'

Cuts at:      0     367     591 
   Size:        367     224
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     240     277     500     591 
   Size:         67     173      37     223      91

  Fragments arranged by size: 

                 223     173      91      67      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167     591 
   Size:        167     424
 
BsrBI CCG'CTC

Cuts at:      0      86     444     591 
   Size:         86     358     147

  Fragments arranged by size: 

                 358     147      86
 
BssHII G'CGCG_C

Cuts at:      0     375     591 
   Size:        375     216
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     387     411     591 
   Size:        387      24     180

  Fragments arranged by size: 

                 387     180      24
 
BstYI r'GATC_y

Cuts at:      0     118     591 
   Size:        118     473
 
BtgI C'CryG_G

Cuts at:      0      56     432     591 
   Size:         56     376     159

  Fragments arranged by size: 

                 376     159      56
 
BtsI GCAGTG_nn'

Cuts at:      0     528     559     591 
   Size:        528      31      32

  Fragments arranged by size: 

                 528      32      31
 
DraIII CAC_nnn'GTG

Cuts at:      0     578     591 
   Size:        578      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     289     591 
   Size:        224      65     302

  Fragments arranged by size: 

                 302     224      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63      64     591 
   Size:         63       1     527

  Fragments arranged by size: 

                 527      63       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     193     225     280     302     312     334     591 
   Size:        193      32      55      22      10      22     257

  Fragments arranged by size: 

                 257     193      55      32      22      22      10
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     591 
   Size:         55     536
 
MspA1I CmG'CkG

Cuts at:      0     263     591 
   Size:        263     328
 
NcoI C'CATG_G

Cuts at:      0      56     591 
   Size:         56     535
 
NspI r_CATG'y

Cuts at:      0     297     591 
   Size:        297     294
 
PciI A'CATG_T

Cuts at:      0     293     591 
   Size:        293     298
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      97     591 
   Size:         97     494
 
PfoI T'CCnGG_A

Cuts at:      0     461     591 
   Size:        461     130
 
PspOMI G'GGCC_C

Cuts at:      0      63     591 
   Size:         63     528
 
PstI C_TGCA'G

Cuts at:      0     566     591 
   Size:        566      25
 
PvuII CAG'CTG

Cuts at:      0     263     591 
   Size:        263     328
 
SfcI C'TryA_G

Cuts at:      0     258     562     591 
   Size:        258     304      29

  Fragments arranged by size: 

                 304     258      29
 
SmlI C'TyrA_G

Cuts at:      0     276     591 
   Size:        276     315
 
StyI C'CwwG_G

Cuts at:      0      56     142     591 
   Size:         56      86     449

  Fragments arranged by size: 

                 449      86      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     337     591 
   Size:        337     254
 
TatI w'GTAC_w

Cuts at:      0     290     591 
   Size:        290     301
 
XmnI GAAnn'nnTTC

Cuts at:      0     367     591 
   Size:        367     224
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI    AlwNI     ApaI    ApaLI     BanI    BanII
     BbsI    BbvCI     BglI Bme1580I     BpmI   Bpu10I    BpuEI     BsaI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI   BssHII
    BstXI    BstYI     BtgI     BtsI   DraIII  Eco57MI EcoO109I    Hin4I
     MmeI   MspA1I     NcoI     NspI     PciI    PflMI     PfoI   PspOMI
     PstI    PvuII     SfcI     SmlI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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