Restriction map for pF1KB9606
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        AspI        AviII       AvrII       
BalI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsmI        BspDI       
BspEI       BspLU11I    BspMI       BsrDI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KspI        MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tru9I       Tsp45I      
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I        818  GGTACC               G^GTACC
AhdI          283  GACGACTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       525  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       818  GGTACC               G^GTACC
AspEI         283  GACGACTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         856  GAGCTC               G[AT]GC[AT]^C
AvaII         987  GGACC                G^G[AT]CC
BamHI         563  GGATCC               G^GATCC
BclI          633  TGATCA               T^GATCA
BcnI          175  CCGGG                CC^[GC]GG
BglI           95  GCCCCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI         1033  CTGCAC               GTGCAG(16/14)
BsiEI         596  CGGTCG               CG[GA][CT]^CG
BsiHKAI       856  GAGCTC               G[AT]GC[AT]^C
BsiWI         809  CGTACG               C^GTACG
Bsp1407I       52  TGTACA               T^GTACA
BspHI         366  TCATGA               T^CATGA
BsrGI          52  TGTACA               T^GTACA
BssHII        996  GCGCGC               G^CGCGC
Eam1105I      283  GACGACTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       584  CCAAGG               C^C[AT][AT]GG
FbaI          633  TGATCA               T^GATCA
HphI          736  GGTGA                GGTGA(8/7)
KpnI          818  GGTACC               GGTAC^C
MaeII         808  ACGT                 A^CGT
NaeI          315  GCCGGC               GCC^GGC
NciI          175  CCGGG                CC^[GC]GG
NgoMIV        315  GCCGGC               G^CCGGC
PshAI         464  GACGCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          366  TCATGA               T^CATGA
SacI          856  GAGCTC               GAGCT^C
SapI           76  GAAGAGC              GCTCTTC(1/4)
SspBI          52  TGTACA               T^GTACA
StuI          230  AGGCCT               AGG^CCT
StyI          584  CCAAGG               C^C[AT][AT]GG
XmnI          525  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI          333  GACGCC               G[GA]^CG[CT]C
              464  GACGCC              
AlwNI          56  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1012  CAGCTGCTG           
AvaI          121  CCCGAG               C^[CT]CG[GA]G
              337  CCCGAG              
BanI          117  GGTGCC               G^G[CT][GA]CC
              818  GGTACC              
BanII         392  GGGCTC               G[GA]GC[CT]^C
              856  GAGCTC              
BfaI           44  CTAG                 C^TAG
              245  CTAG                
BsaHI         333  GACGCC               G[GA]^CG[CT]C
              464  GACGCC              
BsmBI         462  GAGACG               CGTCTC(1/5)
              805  GAGACG              
BsoBI         121  CCCGAG               C^[CT]CG[GA]G
              337  CCCGAG              
BsrBI         112  GAGCGG               CCGCTC(-3/-3)
              814  GAGCGG              
BstXI         159  CCAGTTGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              380  CCAGTAAGTTGG        
BstYI         563  GGATCC               [GA]^GATC[CT]
              654  GGATCT              
DdeI           89  CTGAG                C^T[ACGT]AG
              253  CTGAG               
EaeI            2  TGGCCG               [CT]^GGCC[GA]
              168  TGGCCG              
EcoNI         252  CCTGAGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              397  CCTGCCGCAGG         
HaeII          92  AGCGCC               [GA]GCGC^[CT]
             1053  AGCGCC              
Hin1I         333  GACGCC               G[GA]^CG[CT]C
              464  GACGCC              
MaeI           44  CTAG                 C^TAG
              245  CTAG                
MflI          563  GGATCC               [GA]^GATC[CT]
              654  GGATCT              
PleI          286  GACTC                GAGTC(4/5)
              370  GACTC               
PstI          787  CTGCAG               CTGCA^G
              835  CTGCAG              
PvuII         439  CAGCTG               CAG^CTG
             1012  CAGCTG              
SfcI          787  CTGCAG               C^T[GA][CT]AG
              835  CTGCAG              
TfiI          202  GAATC                G^A[AT]TC
              241  GAATC               
XhoII         563  GGATCC               [GA]^GATC[CT]
              654  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DpnII       EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       ItaI        Ksp632I     
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
TaqI        TseI        TthHB8I     
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