Restriction map for
pF1KB9600
(Linear) (Six-base) MAPSORT of: KIBB9600.seq Check: 2540 from: 1 to: 1119
KIBB9600.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AccI GT'mk_AC
Cuts at: 0 632 1119
Size: 632 487
AfeI AGC'GCT
Cuts at: 0 256 442 451 1004 1119
Size: 256 186 9 553 115
Fragments arranged by size:
553 256 186 115 9
AflIII A'CryG_T
Cuts at: 0 404 1119
Size: 404 715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 1119
Size: 442 677
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 1119
Size: 391 32 696
Fragments arranged by size:
696 391 32
ApaI G_GGCC'C
Cuts at: 0 713 728 807 850 1119
Size: 713 15 79 43 269
Fragments arranged by size:
713 269 79 43 15
AvaI C'yCGr_G
Cuts at: 0 208 360 576 1119
Size: 208 152 216 543
Fragments arranged by size:
543 216 208 152
BanI G'GyrC_C
Cuts at: 0 128 552 580 757 871 1054 1119
Size: 128 424 28 177 114 183 65
Fragments arranged by size:
424 183 177 128 114 65 28
BanII G_rGCy'C
Cuts at: 0 713 728 807 850 1119
Size: 713 15 79 43 269
Fragments arranged by size:
713 269 79 43 15
BbeI G_GCGC'C
Cuts at: 0 132 584 761 875 1058 1119
Size: 132 452 177 114 183 61
Fragments arranged by size:
452 183 177 132 114 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 660 1119
Size: 660 459
BglI GCCn_nnn'nGGC
Cuts at: 0 811 832 854 1119
Size: 811 21 22 265
Fragments arranged by size:
811 265 22 21
BlpI GC'TnA_GC
Cuts at: 0 694 1119
Size: 694 425
Bme1580I G_kGCm'C
Cuts at: 0 557 713 728 807 850 1119
Size: 557 156 15 79 43 269
Fragments arranged by size:
557 269 156 79 43 15
BmgBI CAC'GTC
Cuts at: 0 983 1119
Size: 983 136
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 1119
Size: 387 732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1119
Size: 611 508
Bpu10I CC'TnA_GC
Cuts at: 0 683 1119
Size: 683 436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1119
Size: 319 800
BsaI GGTCTCn'nnnn_
Cuts at: 0 949 1119
Size: 949 170
BsaHI Gr'CG_yC
Cuts at: 0 129 581 646 758 872 1055 1119
Size: 129 452 65 112 114 183 64
Fragments arranged by size:
452 183 129 114 112 65 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 1119
Size: 391 30 698
Fragments arranged by size:
698 391 30
BseYI C'CCAG_C
Cuts at: 0 225 705 927 1005 1119
Size: 225 480 222 78 114
Fragments arranged by size:
480 225 222 114 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1070 1119
Size: 780 290 49
Fragments arranged by size:
780 290 49
BsiEI CG_ry'CG
Cuts at: 0 357 691 811 1119
Size: 357 334 120 308
Fragments arranged by size:
357 334 308 120
BsmBI CGTCTCn'nnnn_
Cuts at: 0 64 1119
Size: 64 1055
Bsp1286I G_dGCh'C
Cuts at: 0 557 713 728 807 850 1119
Size: 557 156 15 79 43 269
Fragments arranged by size:
557 269 156 79 43 15
BsrBI CCG'CTC
Cuts at: 0 14 416 687 693 1119
Size: 14 402 271 6 426
Fragments arranged by size:
426 402 271 14 6
BsrFI r'CCGG_y
Cuts at: 0 352 754 775 810 874 1119
Size: 352 402 21 35 64 245
Fragments arranged by size:
402 352 245 64 35 21
BssHII G'CGCG_C
Cuts at: 0 562 1119
Size: 562 557
BstYI r'GATC_y
Cuts at: 0 338 1119
Size: 338 781
BtgI C'CryG_G
Cuts at: 0 104 111 188 641 959 1119
Size: 104 7 77 453 318 160
Fragments arranged by size:
453 318 160 104 77 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 159 168 180 1119
Size: 159 9 12 939
Fragments arranged by size:
939 159 12 9
BtsI GCAGTG_nn'
Cuts at: 0 885 948 1119
Size: 885 63 171
Fragments arranged by size:
885 171 63
EaeI y'GGCC_r
Cuts at: 0 191 354 614 688 808 812 900 990
Size: 191 163 260 74 120 4 88 90
Cuts at: 990 1119
Size: 129
Fragments arranged by size:
260 191 163 129 120 90 88 74
4
EagI C'GGCC_G
Cuts at: 0 354 688 808 1119
Size: 354 334 120 311
Fragments arranged by size:
354 334 311 120
EarI CTCTTCn'nnn_
Cuts at: 0 53 1119
Size: 53 1066
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 292 741 813 1101 1119
Size: 292 449 72 288 18
Fragments arranged by size:
449 292 288 72 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1119
Size: 611 508
EcoO109I rG'GnC_Cy
Cuts at: 0 90 709 724 803 847 913 1119
Size: 90 619 15 79 44 66 206
Fragments arranged by size:
619 206 90 79 66 44 15
FseI GG_CCGG'CC
Cuts at: 0 356 814 1119
Size: 356 458 305
Fragments arranged by size:
458 356 305
HaeII r_GCGC'y
Cuts at: 0 132 141 216 258 385 444 453 584
Size: 132 9 75 42 127 59 9 131
Cuts at: 584 761 875 1006 1058 1119
Size: 177 114 131 52 61
Fragments arranged by size:
177 132 131 131 127 114 75 61
59 52 42 9 9
KasI G'GCGC_C
Cuts at: 0 128 580 757 871 1054 1119
Size: 128 452 177 114 183 65
Fragments arranged by size:
452 183 177 128 114 65
MscI TGG'CCA
Cuts at: 0 193 1119
Size: 193 926
MslI CAynn'nnrTG
Cuts at: 0 187 1119
Size: 187 932
MspA1I CmG'CkG
Cuts at: 0 113 643 895 931 1119
Size: 113 530 252 36 188
Fragments arranged by size:
530 252 188 113 36
NaeI GCC'GGC
Cuts at: 0 354 756 812 876 1119
Size: 354 402 56 64 243
Fragments arranged by size:
402 354 243 64 56
NarI GG'CG_CC
Cuts at: 0 129 581 758 872 1055 1119
Size: 129 452 177 114 183 64
Fragments arranged by size:
452 183 177 129 114 64
NcoI C'CATG_G
Cuts at: 0 188 1119
Size: 188 931
NgoMIV G'CCGG_C
Cuts at: 0 352 754 810 874 1119
Size: 352 402 56 64 245
Fragments arranged by size:
402 352 245 64 56
NotI GC'GGCC_GC
Cuts at: 0 688 1119
Size: 688 431
NspI r_CATG'y
Cuts at: 0 408 483 1119
Size: 408 75 636
Fragments arranged by size:
636 408 75
PasI CC'CwG_GG
Cuts at: 0 94 1119
Size: 94 1025
PciI A'CATG_T
Cuts at: 0 404 1119
Size: 404 715
PflMI CCAn_nnn'nTGG
Cuts at: 0 287 1119
Size: 287 832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 1119
Size: 391 32 696
Fragments arranged by size:
696 391 32
PpuMI rG'GwC_Cy
Cuts at: 0 90 913 1119
Size: 90 823 206
Fragments arranged by size:
823 206 90
PspOMI G'GGCC_C
Cuts at: 0 709 724 803 846 1119
Size: 709 15 79 43 273
Fragments arranged by size:
709 273 79 43 15
PstI C_TGCA'G
Cuts at: 0 149 1119
Size: 149 970
SacII CC_GC'GG
Cuts at: 0 114 644 1119
Size: 114 530 475
Fragments arranged by size:
530 475 114
SanDI GG'GwC_CC
Cuts at: 0 90 913 1119
Size: 90 823 206
Fragments arranged by size:
823 206 90
SapI GCTCTTCn'nnn_
Cuts at: 0 53 1119
Size: 53 1066
SfcI C'TryA_G
Cuts at: 0 145 165 1119
Size: 145 20 954
Fragments arranged by size:
954 145 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 811 1119
Size: 811 308
SfoI GGC'GCC
Cuts at: 0 130 582 759 873 1056 1119
Size: 130 452 177 114 183 63
Fragments arranged by size:
452 183 177 130 114 63
SgrAI Cr'CCGG_yG
Cuts at: 0 754 1119
Size: 754 365
SmaI CCC'GGG
Cuts at: 0 362 578 1119
Size: 362 216 541
Fragments arranged by size:
541 362 216
SmlI C'TyrA_G
Cuts at: 0 334 1119
Size: 334 785
StyI C'CwwG_G
Cuts at: 0 188 1119
Size: 188 931
XmaI C'CCGG_G
Cuts at: 0 360 576 1119
Size: 360 216 543
Fragments arranged by size:
543 360 216
Enzymes that do cut:
AccI AfeI AflIII AlfI AloI ApaI AvaI BanI
BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseYI BsgI
BsiEI BsmBI Bsp1286I BsrBI BsrFI BssHII BstYI BtgI
BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I
FseI HaeII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PpiI
PpuMI PspOMI PstI SacII SanDI SapI SfcI SfiI
SfoI SgrAI SmaI SmlI StyI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BmtI BplI BsaAI BsaBI BsaWI BseRI BsiHKAI BsiWI
BsmI BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NruI NsiI PacI PfoI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SalI SbfI ScaI SexAI SgfI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI