Restriction map for
pF1KB9587
(Circular) (Six-base) MAPSORT of: pF1KB9587.seq Check: 1778 from: 1 to: 3821
pF1KB9587 3821 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 396 396
Size: 3821
AccI GT'mk_AC
Cuts at: 170 600 859 170
Size: 430 259 3132
Fragments arranged by size:
3132 430 259
Acc65I G'GTAC_C
Cuts at: 837 837
Size: 3821
AclI AA'CG_TT
Cuts at: 3661 3661
Size: 3821
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1646 2078 2825 3093 145
Size: 1501 432 747 268 873
Fragments arranged by size:
1501 873 747 432 268
AflIII A'CryG_T
Cuts at: 1173 2277 3254 1173
Size: 1104 977 1740
Fragments arranged by size:
1740 1104 977
AgeI A'CCGG_T
Cuts at: 2209 2209
Size: 3821
AleI CACnn'nnGTG
Cuts at: 202 202
Size: 3821
AlwNI CAG_nnn'CTG
Cuts at: 592 679 700 789 1126 2693 592
Size: 87 21 89 337 1567 1720
Fragments arranged by size:
1720 1567 337 89 87 21
ApaI G_GGCC'C
Cuts at: 586 586
Size: 3821
ApaLI G'TGCA_C
Cuts at: 2591 2591
Size: 3821
ApoI r'AATT_y
Cuts at: 825 3174 3430 825
Size: 2349 256 1216
Fragments arranged by size:
2349 1216 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3821
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3821
AvaI C'yCGr_G
Cuts at: 841 841
Size: 3821
BamHI G'GATC_C
Cuts at: 846 846
Size: 3821
BanI G'GyrC_C
Cuts at: 213 302 351 565 669 837 1251 1499 1534
Size: 89 49 214 104 168 414 248 35
Cuts at: 1534 213
Size: 2500
Fragments arranged by size:
2500 414 248 214 168 104 89 49
35
BanII G_rGCy'C
Cuts at: 586 835 1865 586
Size: 249 1030 2542
Fragments arranged by size:
2542 1030 249
BbeI G_GCGC'C
Cuts at: 1255 1503 1255
Size: 248 3573
BbvCI CC'TCA_GC
Cuts at: 472 472
Size: 3821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3787
BciVI GTATCCnnnnn_n'
Cuts at: 1719 2480 3821 1719
Size: 761 1341 1719
Fragments arranged by size:
1719 1341 761
BclI T'GATC_A
Cuts at: 1341 1341
Size: 3821
BglI GCCn_nnn'nGGC
Cuts at: 451 451
Size: 3821
BglII A'GATC_T
Cuts at: 1336 3373 1336
Size: 2037 1784
BlpI GC'TnA_GC
Cuts at: 112 933 112
Size: 821 3000
Bme1580I G_kGCm'C
Cuts at: 586 672 781 1446 1539 2595 586
Size: 86 109 665 93 1056 1812
Fragments arranged by size:
1812 1056 665 109 93 86
BmrI ACTGGGnnnn_n'
Cuts at: 392 1033 1114 1449 3593 392
Size: 641 81 335 2144 620
Fragments arranged by size:
2144 641 620 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 515 2188 515
Size: 1673 2148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 811 484
Size: 327 3494
Bpu10I CC'TnA_GC
Cuts at: 472 472
Size: 3821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 637 997 1876 2368 2666 244
Size: 393 360 879 492 298 1399
Fragments arranged by size:
1399 879 492 393 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 3525 3525
Size: 3821
BsaAI yAC'GTr
Cuts at: 1174 1174
Size: 3821
BsaBI GATnn'nnATC
Cuts at: 6 1335 3372 6
Size: 1329 2037 455
Fragments arranged by size:
2037 1329 455
BsaHI Gr'CG_yC
Cuts at: 343 1252 1500 2202 3698 343
Size: 909 248 702 1496 466
Fragments arranged by size:
1496 909 702 466 248
BsaWI w'CCGG_w
Cuts at: 729 1010 1531 2209 2483 2630 3041 729
Size: 281 521 678 274 147 411 1509
Fragments arranged by size:
1509 678 521 411 281 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 157 160 163 154
Size: 3 3 3 3812
Fragments arranged by size:
3812 3 3 3
BseYI C'CCAG_C
Cuts at: 556 579 585 1246 2004 2581 3077 556
Size: 23 6 661 758 577 496 1300
Fragments arranged by size:
1300 758 661 577 496 23 6
BsiEI CG_ry'CG
Cuts at: 104 224 799 1363 1409 2617 104
Size: 120 575 564 46 1208 1308
Fragments arranged by size:
1308 1208 575 564 120 46
BsiHKAI G_wGCw'C
Cuts at: 260 835 1613 1803 2595 260
Size: 575 778 190 792 1486
Fragments arranged by size:
1486 792 778 575 190
Bsp1286I G_dGCh'C
Cuts at: 260 586 672 781 835 1446 1539 1613 1803
Size: 326 86 109 54 611 93 74 190
Cuts at: 1803 1865 2595 260
Size: 62 730 1486
Fragments arranged by size:
1486 730 611 326 190 109 93 86
74 62 54
BspEI T'CCGG_A
Cuts at: 729 3041 729
Size: 2312 1509
BspHI T'CATG_A
Cuts at: 2997 2997
Size: 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 396 584 871 1085 1387 1768 305
Size: 91 188 287 214 302 381 2358
Fragments arranged by size:
2358 381 302 287 214 188 91
BsrBI CCG'CTC
Cuts at: 50 2113 2167 3651 3816 50
Size: 2063 54 1484 165 55
Fragments arranged by size:
2063 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1733 1733
Size: 3821
BsrFI r'CCGG_y
Cuts at: 1819 2000 2209 1819
Size: 181 209 3431
Fragments arranged by size:
3431 209 181
BssSI C'ACGA_G
Cuts at: 2092 2450 2092
Size: 358 3463
BstAPI GCAn_nnn'nTGC
Cuts at: 309 1198 309
Size: 889 2932
BstBI TT'CG_AA
Cuts at: 2182 2182
Size: 3821
BstXI CCAn_nnnn'nTGG
Cuts at: 462 462
Size: 3821
BstYI r'GATC_y
Cuts at: 1 186 846 1313 1336 1671 1917 2929 3015
Size: 185 660 467 23 335 246 1012 86
Cuts at: 3015 3027 3373 1
Size: 12 346 449
Fragments arranged by size:
1012 660 467 449 346 335 246 185
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 701 701
Size: 3821
BtgI C'CryG_G
Cuts at: 224 347 356 449 224
Size: 123 9 93 3596
Fragments arranged by size:
3596 123 93 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1771 1952 2115 1771
Size: 181 163 3477
Fragments arranged by size:
3477 181 163
BtsI GCAGTG_nn'
Cuts at: 1064 1064
Size: 3821
DraI TTT'AAA
Cuts at: 820 820
Size: 3821
DraIII CAC_nnn'GTG
Cuts at: 309 309
Size: 3821
DrdI GACnn_nn'nnGTC
Cuts at: 1527 2385 1527
Size: 858 2963
EaeI y'GGCC_r
Cuts at: 192 221 452 1406 1580 1971 1998 3343 192
Size: 29 231 954 174 391 27 1345 670
Fragments arranged by size:
1345 954 670 391 231 174 29 27
EagI C'GGCC_G
Cuts at: 221 1406 221
Size: 1185 2636
EarI CTCTTCn'nnn_
Cuts at: 1844 2054 1844
Size: 210 3611
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2029 2339 2485 3631 2029
Size: 310 146 1146 2219
Fragments arranged by size:
2219 1146 310 146
EcoICRI GAG'CTC
Cuts at: 833 833
Size: 3821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 484 811 1646 2078 2825 3093 145
Size: 339 327 835 432 747 268 873
Fragments arranged by size:
873 835 747 432 339 327 268
EcoNI CCTnn'n_nnAGG
Cuts at: 463 493 628 463
Size: 30 135 3656
Fragments arranged by size:
3656 135 30
EcoO109I rG'GnC_Cy
Cuts at: 468 486 621 684 697 960 468
Size: 18 135 63 13 263 3329
Fragments arranged by size:
3329 263 135 63 18 13
EcoRI G'AATT_C
Cuts at: 825 825
Size: 3821
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1840 1840
Size: 3821
FspI TGC'GCA
Cuts at: 1602 1602
Size: 3821
HaeII r_GCGC'y
Cuts at: 556 1255 1503 2525 3504 556
Size: 699 248 1022 979 873
Fragments arranged by size:
1022 979 873 699 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 1663 1693 1695 1725 105
Size: 32 1526 30 2 30 2201
Fragments arranged by size:
2201 1526 32 30 30 2
HincII GTy'rAC
Cuts at: 171 860 171
Size: 689 3132
KasI G'GCGC_C
Cuts at: 1251 1499 1251
Size: 248 3573
KpnI G_GTAC'C
Cuts at: 841 841
Size: 3821
MluI A'CGCG_T
Cuts at: 3254 3254
Size: 3821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2676 2492
Size: 184 3637
MscI TGG'CCA
Cuts at: 194 454 1582 194
Size: 260 1128 2433
Fragments arranged by size:
2433 1128 260
MslI CAynn'nnrTG
Cuts at: 202 646 766 1810 1937 202
Size: 444 120 1044 127 2086
Fragments arranged by size:
2086 1044 444 127 120
MspA1I CmG'CkG
Cuts at: 202 358 932 1246 1606 2619 2864 202
Size: 156 574 314 360 1013 245 1159
Fragments arranged by size:
1159 1013 574 360 314 245 156
NaeI GCC'GGC
Cuts at: 2002 2002
Size: 3821
NarI GG'CG_CC
Cuts at: 1252 1500 1252
Size: 248 3573
NcoI C'CATG_G
Cuts at: 347 449 347
Size: 102 3719
NgoMIV G'CCGG_C
Cuts at: 2000 2000
Size: 3821
NspI r_CATG'y
Cuts at: 874 1374 2281 874
Size: 500 907 2414
Fragments arranged by size:
2414 907 500
PciI A'CATG_T
Cuts at: 2277 2277
Size: 3821
PflMI CCAn_nnn'nTGG
Cuts at: 510 592 510
Size: 82 3739
PfoI T'CCnGG_A
Cuts at: 461 461
Size: 3821
PmeI GTTT'AAAC
Cuts at: 820 820
Size: 3821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 3789
PpuMI rG'GwC_Cy
Cuts at: 468 468
Size: 3821
PspOMI G'GGCC_C
Cuts at: 582 582
Size: 3821
PstI C_TGCA'G
Cuts at: 281 393 868 1553 281
Size: 112 475 685 2549
Fragments arranged by size:
2549 685 475 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3821
PvuII CAG'CTG
Cuts at: 1246 1606 1246
Size: 360 3461
RsrII CG'GwC_CG
Cuts at: 2016 2016
Size: 3821
SacI G_AGCT'C
Cuts at: 835 835
Size: 3821
SacII CC_GC'GG
Cuts at: 359 359
Size: 3821
SalI G'TCGA_C
Cuts at: 169 858 169
Size: 689 3132
SapI GCTCTTCn'nnn_
Cuts at: 1844 2054 1844
Size: 210 3611
SbfI CC_TGCA'GG
Cuts at: 868 868
Size: 3821
ScaI AGT'ACT
Cuts at: 3338 3338
Size: 3821
SexAI A'CCwGG_T
Cuts at: 305 305
Size: 3821
SfcI C'TryA_G
Cuts at: 33 277 389 864 1549 2542 2733 33
Size: 244 112 475 685 993 191 1121
Fragments arranged by size:
1121 993 685 475 244 191 112
SfoI GGC'GCC
Cuts at: 1253 1501 1253
Size: 248 3573
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3821
SmaI CCC'GGG
Cuts at: 843 843
Size: 3821
SmlI C'TyrA_G
Cuts at: 259 616 976 1891 2383 2645 259
Size: 357 360 915 492 262 1435
Fragments arranged by size:
1435 915 492 360 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3821
SphI G_CATG'C
Cuts at: 874 1374 874
Size: 500 3321
StyI C'CwwG_G
Cuts at: 347 449 509 569 955 1309 347
Size: 102 60 60 386 354 2859
Fragments arranged by size:
2859 386 354 102 60 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 553 1404 1537 1996 2205 553
Size: 851 133 459 209 2169
Fragments arranged by size:
2169 851 459 209 133
TatI w'GTAC_w
Cuts at: 3336 3336
Size: 3821
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 3789
Tth111I GACn'n_nGTC
Cuts at: 1618 1618
Size: 3821
XbaI T'CTAG_A
Cuts at: 852 852
Size: 3821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1316 1316
Size: 3821
XmaI C'CCGG_G
Cuts at: 841 841
Size: 3821
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI