Restriction map for pF1KB9584
 (Linear) (Six-base) MAPSORT of: KIBB9584.seq  Check: 3835  from: 1  to: 810



KIBB9584.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     361     437     810 
   Size:        361      76     373

  Fragments arranged by size: 

                 373     361      76
 
AvaI C'yCGr_G

Cuts at:      0      99     249     507     546     810 
   Size:         99     150     258      39     264

  Fragments arranged by size: 

                 264     258     150      99      39
 
BanI G'GyrC_C

Cuts at:      0      46     242     810 
   Size:         46     196     568

  Fragments arranged by size: 

                 568     196      46
 
BanII G_rGCy'C

Cuts at:      0     106     810 
   Size:        106     704
 
BglI GCCn_nnn'nGGC

Cuts at:      0     268     320     613     810 
   Size:        268      52     293     197

  Fragments arranged by size: 

                 293     268     197      52
 
Bme1580I G_kGCm'C

Cuts at:      0     245     810 
   Size:        245     565
 
BmgBI CAC'GTC

Cuts at:      0     621     810 
   Size:        621     189
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     282     810 
   Size:        282     528
 
BseYI C'CCAG_C

Cuts at:      0     131     741     793     810 
   Size:        131     610      52      17

  Fragments arranged by size: 

                 610     131      52      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     810 
   Size:         43     767
 
BsiHKAI G_wGCw'C

Cuts at:      0     164     810 
   Size:        164     646
 
BsmI GAATG_Cn'

Cuts at:      0     195     810 
   Size:        195     615
 
Bsp1286I G_dGCh'C

Cuts at:      0     106     164     245     810 
   Size:        106      58      81     565

  Fragments arranged by size: 

                 565     106      81      58
 
BsrBI CCG'CTC

Cuts at:      0     200     810 
   Size:        200     610
 
BsrDI GCAATG_nn'

Cuts at:      0     638     810 
   Size:        638     172
 
BsrFI r'CCGG_y

Cuts at:      0     330     810 
   Size:        330     480
 
BssHII G'CGCG_C

Cuts at:      0     254     810 
   Size:        254     556
 
BssSI C'ACGA_G

Cuts at:      0     195     810 
   Size:        195     615
 
BtgI C'CryG_G

Cuts at:      0      27     266     611     810 
   Size:         27     239     345     199

  Fragments arranged by size: 

                 345     239     199      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     229     810 
   Size:        229     581
 
EaeI y'GGCC_r

Cuts at:      0      17     269     308     332     810 
   Size:         17     252      39      24     478

  Fragments arranged by size: 

                 478     252      39      24      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     422     810 
   Size:        422     388
 
EcoO109I rG'GnC_Cy

Cuts at:      0     298     810 
   Size:        298     512
 
HaeII r_GCGC'y

Cuts at:      0      36     810 
   Size:         36     774
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      93     125     810 
   Size:         93      32     685

  Fragments arranged by size: 

                 685      93      32
 
MscI TGG'CCA

Cuts at:      0      19     310     810 
   Size:         19     291     500

  Fragments arranged by size: 

                 500     291      19
 
MslI CAynn'nnrTG

Cuts at:      0      85     810 
   Size:         85     725
 
MspA1I CmG'CkG

Cuts at:      0     268     345     482     575     587     593     628     810 
   Size:        268      77     137      93      12       6      35     182

  Fragments arranged by size: 

                 268     182     137      93      77      35      12       6
            
 
NaeI GCC'GGC

Cuts at:      0     332     810 
   Size:        332     478
 
NdeI CA'TA_TG

Cuts at:      0      11     810 
   Size:         11     799
 
NgoMIV G'CCGG_C

Cuts at:      0     330     810 
   Size:        330     480
 
NspI r_CATG'y

Cuts at:      0     214     342     405     603     810 
   Size:        214     128      63     198     207

  Fragments arranged by size: 

                 214     207     198     128      63
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      15      81     294     810 
   Size:         15      66     213     516

  Fragments arranged by size: 

                 516     213      66      15
 
PpuMI rG'GwC_Cy

Cuts at:      0     298     810 
   Size:        298     512
 
PstI C_TGCA'G

Cuts at:      0     350     426     810 
   Size:        350      76     384

  Fragments arranged by size: 

                 384     350      76
 
PvuII CAG'CTG

Cuts at:      0     345     810 
   Size:        345     465
 
SacII CC_GC'GG

Cuts at:      0     269     810 
   Size:        269     541
 
SanDI GG'GwC_CC

Cuts at:      0     298     810 
   Size:        298     512
 
SfcI C'TryA_G

Cuts at:      0     346     422     810 
   Size:        346      76     388

  Fragments arranged by size: 

                 388     346      76
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     268     810 
   Size:        268     542
 
SphI G_CATG'C

Cuts at:      0     214     342     810 
   Size:        214     128     468

  Fragments arranged by size: 

                 468     214     128
 
StyI C'CwwG_G

Cuts at:      0     689     810 
   Size:        689     121
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     763     795     810 
   Size:        763      32      15

  Fragments arranged by size: 

                 763      32      15
 

 Enzymes that do cut:

     AlfI     AvaI     BanI    BanII     BglI Bme1580I    BmgBI     BmrI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrDI    BsrFI
   BssHII    BssSI     BtgI    BtgZI     EaeI    EcoNI EcoO109I    HaeII
    Hin4I     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV     NspI
    PflMI    PpuMI     PstI    PvuII    SacII    SanDI     SfcI     SfiI
     SphI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NcoI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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