Restriction map for pF1KB9581
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# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     FbaI        
FseI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MscI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tru9I       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII        778  TCCGGA               T^CCGGA
AfaI          852  GTAC                 GT^AC
AlwNI         836  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          437  GGGCCC               GGGCC^C
AvaII         998  GGTCC                G^G[AT]CC
AviII         545  TGCGCA               TGC^GCA
BfaI          484  CTAG                 C^TAG
BlpI          766  GCTCAGC              GC^T[ACGT]AGC
BpmI          680  CTCCAG               CTGGAG(16/14)
Bpu1102I      766  GCTCAGC              GC^T[ACGT]AGC
BsaWI         778  TCCGGA               [AT]^CCGG[AT]
BseAI         778  TCCGGA               T^CCGGA
BseRI         321  GAGGAG               GAGGAG(10/8)
BsmBI         477  GAGACG               CGTCTC(1/5)
BspEI         778  TCCGGA               T^CCGGA
BspMI         111  ACCTGC               ACCTGC(4/8)
BsrBI         502  GAGCGG               CCGCTC(-3/-3)
BsrDI         548  GCAATG               GCAATG(2/0)
BsrFI         360  GCCGGT               [GA]^CCGG[CT]
BstAPI        960  GCAGCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         181  CCACGCGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         766  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        360  GCCGGT               [GA]^CCGG[CT]
DraII         437  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          719  CCGCGG               C^C[GA][CT]GG
EarI           22  GAAGAG               CTCTTC(1/4)
EcoO109I      437  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       514  ATGCAT               ATGCA^T
FspI          545  TGCGCA               TGC^GCA
HaeII         898  AGCGCC               [GA]GCGC^[CT]
HgaI          868  GACGC                GACGC(5/10)
Ksp632I        22  GAAGAG               CTCTTC(1/4)
KspI          719  CCGCGG               CCGC^GG
MaeI          484  CTAG                 C^TAG
MaeIII       1043  GTGAC                ^GT[ACGT]AC
MboII          22  GAAGA                GAAGA(8/7)
MroI          778  TCCGGA               T^CCGGA
MslI         1036  CATTACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI          483  GCTAGC               G^CTAGC
NsiI          514  ATGCAT               ATGCA^T
NspI           15  GCATGC               [GA]CATG^[CT]
RsaI          852  GTAC                 GT^AC
SacII         719  CCGCGG               CCGC^GG
SphI           15  GCATGC               GCATG^C
Tsp45I       1043  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          142  GTCTAC               GT^[AC][GT]AC
              409  GTAGAC              
AcsI          927  GAATTT               [GA]^AATT[CT]
             1018  GAATTT              
ApoI          927  GAATTT               [GA]^AATT[CT]
             1018  GAATTT              
BanI          262  GGTGCC               G^G[CT][GA]CC
              334  GGTGCC              
BglI          187  GCCTGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              265  GCCTCAGAGGC         
BsaI           50  GAGACC               GGTCTC(1/5)
              595  GAGACC              
BsrI          234  CCAGT                ACTGG(1/-1)
              964  CCAGT               
EaeI          300  CGGCCG               [CT]^GGCC[GA]
              793  CGGCCG              
EagI          300  CGGCCG               C^GGCCG
              793  CGGCCG              
EclXI         300  CGGCCG               C^GGCCG
              793  CGGCCG              
Eco52I        300  CGGCCG               C^GGCCG
              793  CGGCCG              
HinfI         819  GACTC                G^A[ACGT]TC
             1045  GACTC               
HphI          363  GGTGA                GGTGA(8/7)
              456  GGTGA               
NlaIII         16  CATG                 CATG^
              517  CATG                
PleI          819  GACTC                GAGTC(4/5)
             1045  GACTC               
PstI          423  CTGCAG               CTGCA^G
              601  CTGCAG              
SfcI          423  CTGCAG               C^T[GA][CT]AG
              601  CTGCAG              
Tsp509I       928  AATT                 ^AATT
             1019  AATT                
TspRI         965  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1041  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        BanII       
BbvI        BcnI        BmyI        BsaJI       BsgI        
BsiEI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       PvuII       Sau96I      
ScrFI       SfaNI       SmaI        TaqI        TseI        
TthHB8I     XmaCI       XmaI        
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