Restriction map for pF1KB9581
 (Linear) (Six-base) MAPSORT of: KIBB9581.seq  Check: 8233  from: 1  to: 1062



KIBB9581.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     120    1062 
   Size:        120     942
 
AccI GT'mk_AC

Cuts at:      0     143     410    1062 
   Size:        143     267     652

  Fragments arranged by size: 

                 652     267     143
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53    1062 
   Size:         53    1009
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     128    1062 
   Size:        128     934
 
AlwNI CAG_nnn'CTG

Cuts at:      0     841    1062 
   Size:        841     221
 
ApaI G_GGCC'C

Cuts at:      0     441    1062 
   Size:        441     621
 
ApoI r'AATT_y

Cuts at:      0     927    1018    1062 
   Size:        927      91      44

  Fragments arranged by size: 

                 927      91      44
 
AvaI C'yCGr_G

Cuts at:      0     284     355     805     946    1062 
   Size:        284      71     450     141     116

  Fragments arranged by size: 

                 450     284     141     116      71
 
BanI G'GyrC_C

Cuts at:      0     262     334    1062 
   Size:        262      72     728

  Fragments arranged by size: 

                 728     262      72
 
BanII G_rGCy'C

Cuts at:      0     355     441     768    1062 
   Size:        355      86     327     294

  Fragments arranged by size: 

                 355     327     294      86
 
BfrBI ATG'CAT

Cuts at:      0     516    1062 
   Size:        516     546
 
BglI GCCn_nnn'nGGC

Cuts at:      0     193     271    1062 
   Size:        193      78     791

  Fragments arranged by size: 

                 791     193      78
 
BlpI GC'TnA_GC

Cuts at:      0     767    1062 
   Size:        767     295
 
Bme1580I G_kGCm'C

Cuts at:      0     441    1062 
   Size:        441     621
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     228    1062 
   Size:        228     834
 
BmtI G_CTAG'C

Cuts at:      0     487    1062 
   Size:        487     575
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    1062 
   Size:        665     397
 
Bpu10I CC'TnA_GC

Cuts at:      0     152    1062 
   Size:        152     910
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      44     589    1062 
   Size:         44     545     473

  Fragments arranged by size: 

                 545     473      44
 
BsaWI w'CCGG_w

Cuts at:      0     778    1062 
   Size:        778     284
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     336    1062 
   Size:        336     726
 
BseYI C'CCAG_C

Cuts at:      0      68     258     417     652     760     834    1062 
   Size:         68     190     159     235     108      74     228

  Fragments arranged by size: 

                 235     228     190     159     108      74      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     487     859     880     988    1062 
   Size:        201     286     372      21     108      74

  Fragments arranged by size: 

                 372     286     201     108      74      21
 
BsiEI CG_ry'CG

Cuts at:      0     303     755     796    1062 
   Size:        303     452      41     266

  Fragments arranged by size: 

                 452     303     266      41
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     471    1062 
   Size:        471     591
 
Bsp1286I G_dGCh'C

Cuts at:      0     355     441     768    1062 
   Size:        355      86     327     294

  Fragments arranged by size: 

                 355     327     294      86
 
BspEI T'CCGG_A

Cuts at:      0     778    1062 
   Size:        778     284
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     120    1062 
   Size:        120     942
 
BsrBI CCG'CTC

Cuts at:      0     504    1062 
   Size:        504     558
 
BsrDI GCAATG_nn'

Cuts at:      0     555    1062 
   Size:        555     507
 
BsrFI r'CCGG_y

Cuts at:      0     360    1062 
   Size:        360     702
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     966    1062 
   Size:        966      96
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     188    1062 
   Size:        188     874
 
BtgI C'CryG_G

Cuts at:      0     719    1062 
   Size:        719     343
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      43     925    1062 
   Size:         43     882     137

  Fragments arranged by size: 

                 882     137      43
 
EaeI y'GGCC_r

Cuts at:      0     300     793    1062 
   Size:        300     493     269

  Fragments arranged by size: 

                 493     300     269
 
EagI C'GGCC_G

Cuts at:      0     300     793    1062 
   Size:        300     493     269

  Fragments arranged by size: 

                 493     300     269
 
EarI CTCTTCn'nnn_

Cuts at:      0      17    1062 
   Size:         17    1045
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     665    1062 
   Size:         53     612     397

  Fragments arranged by size: 

                 612     397      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0     438    1062 
   Size:        438     624
 
FspI TGC'GCA

Cuts at:      0     547    1062 
   Size:        547     515
 
HaeII r_GCGC'y

Cuts at:      0     902    1062 
   Size:        902     160
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     120     152    1062 
   Size:        120      32     910

  Fragments arranged by size: 

                 910     120      32
 
MslI CAynn'nnrTG

Cuts at:      0    1040    1062 
   Size:       1040      22
 
MspA1I CmG'CkG

Cuts at:      0     277     329     381     543     656     721     760     792 
   Size:        277      52      52     162     113      65      39      32

Cuts at:    792     864    1062 
   Size:         72     198

  Fragments arranged by size: 

                 277     198     162     113      72      65      52      52
                  39      32
 
NheI G'CTAG_C

Cuts at:      0     483    1062 
   Size:        483     579
 
NsiI A_TGCA'T

Cuts at:      0     518    1062 
   Size:        518     544
 
NspI r_CATG'y

Cuts at:      0      19    1062 
   Size:         19    1043
 
PfoI T'CCnGG_A

Cuts at:      0     164    1062 
   Size:        164     898
 
PspOMI G'GGCC_C

Cuts at:      0     437    1062 
   Size:        437     625
 
PstI C_TGCA'G

Cuts at:      0     427     605    1062 
   Size:        427     178     457

  Fragments arranged by size: 

                 457     427     178
 
PvuII CAG'CTG

Cuts at:      0     381     543     760     864    1062 
   Size:        381     162     217     104     198

  Fragments arranged by size: 

                 381     217     198     162     104
 
SacII CC_GC'GG

Cuts at:      0     722    1062 
   Size:        722     340
 
SfcI C'TryA_G

Cuts at:      0     423     601    1062 
   Size:        423     178     461

  Fragments arranged by size: 

                 461     423     178
 
SmaI CCC'GGG

Cuts at:      0     286     357     807     948    1062 
   Size:        286      71     450     141     114

  Fragments arranged by size: 

                 450     286     141     114      71
 
SphI G_CATG'C

Cuts at:      0      19    1062 
   Size:         19    1043
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     250     986    1062 
   Size:        250     736      76

  Fragments arranged by size: 

                 736     250      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     367     399    1062 
   Size:        367      32     663

  Fragments arranged by size: 

                 663     367      32
 
XmaI C'CCGG_G

Cuts at:      0     284     355     805     946    1062 
   Size:        284      71     450     141     116

  Fragments arranged by size: 

                 450     284     141     116      71
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI
     BpmI   Bpu10I     BsaI    BsaWI    BseRI    BseYI     BsgI    BsiEI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI
    BstXI     BtgI    BtgZI     EaeI     EagI     EarI  Eco57MI EcoO109I
     FspI    HaeII    Hin4I     MslI   MspA1I     NheI     NsiI     NspI
     PfoI   PspOMI     PstI    PvuII    SacII     SfcI     SmaI     SphI
    TaqII     TstI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BglII
    BmgBI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI  BsiHKAI
    BsiWI     BsmI    BspHI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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