(Circular) (Six-base) MAPSORT of: pF1KB9573.seq Check: 1099 from: 1 to: 4238 pF1KB9573 4238 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AccI GT'mk_AC Cuts at: 1276 1276 Size: 4238 Acc65I G'GTAC_C Cuts at: 1254 1254 Size: 4238 AclI AA'CG_TT Cuts at: 4078 4078 Size: 4238 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 821 2063 2495 2848 3510 821 Size: 1242 432 353 662 1549 Fragments arranged by size: 1549 1242 662 432 353 AflIII A'CryG_T Cuts at: 1590 3390 3671 1590 Size: 1800 281 2157 Fragments arranged by size: 2157 1800 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 700 700 Size: 4238 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 756 788 756 Size: 32 4206 AlwNI CAG_nnn'CTG Cuts at: 463 1543 2981 463 Size: 1080 1438 1720 Fragments arranged by size: 1720 1438 1080 ApaLI G'TGCA_C Cuts at: 3076 3076 Size: 4238 ApoI r'AATT_y Cuts at: 559 648 779 1242 3591 3847 559 Size: 89 131 463 2349 256 950 Fragments arranged by size: 2349 950 463 256 131 89 AseI AT'TA_AT Cuts at: 20 20 Size: 4238 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4238 AvaI C'yCGr_G Cuts at: 1258 1258 Size: 4238 BamHI G'GATC_C Cuts at: 1263 1263 Size: 4238 BanI G'GyrC_C Cuts at: 1254 1668 1916 1951 1254 Size: 414 248 35 3541 Fragments arranged by size: 3541 414 248 35 BanII G_rGCy'C Cuts at: 1252 2282 1252 Size: 1030 3208 BbeI G_GCGC'C Cuts at: 1672 1920 1672 Size: 248 3990 BbsI GAAGACnn'nnnn_ Cuts at: 827 827 Size: 4238 BciVI GTATCCnnnnn_n' Cuts at: 380 2136 3192 4238 380 Size: 1756 1056 1046 380 Fragments arranged by size: 1756 1056 1046 380 BclI T'GATC_A Cuts at: 1758 1758 Size: 4238 BfrBI ATG'CAT Cuts at: 660 660 Size: 4238 BglII A'GATC_T Cuts at: 1753 3790 1753 Size: 2037 2201 BlpI GC'TnA_GC Cuts at: 758 1350 758 Size: 592 3646 Bme1580I G_kGCm'C Cuts at: 528 1863 1956 3080 528 Size: 1335 93 1124 1686 Fragments arranged by size: 1686 1335 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 1450 1531 1866 4010 1450 Size: 81 335 2144 1678 Fragments arranged by size: 2144 1678 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 4238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1414 2293 3007 3305 1414 Size: 879 714 298 2347 Fragments arranged by size: 2347 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 3942 3942 Size: 4238 BsaAI yAC'GTr Cuts at: 1591 1591 Size: 4238 BsaBI GATnn'nnATC Cuts at: 6 1752 3789 6 Size: 1746 2037 455 Fragments arranged by size: 2037 1746 455 BsaHI Gr'CG_yC Cuts at: 1669 1917 2619 4115 1669 Size: 248 702 1496 1792 Fragments arranged by size: 1792 1496 702 248 BsaWI w'CCGG_w Cuts at: 1427 1948 2626 3037 3184 3458 1427 Size: 521 678 411 147 274 2207 Fragments arranged by size: 2207 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 211 220 244 247 250 253 256 880 211 Size: 9 24 3 3 3 3 624 3569 Fragments arranged by size: 3569 624 24 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 152 1663 2421 3086 3494 152 Size: 1511 758 665 408 896 Fragments arranged by size: 1511 896 758 665 408 BsiEI CG_ry'CG Cuts at: 104 1780 1826 3056 104 Size: 1676 46 1230 1286 Fragments arranged by size: 1676 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1252 2030 2220 3080 1252 Size: 778 190 860 2410 Fragments arranged by size: 2410 860 778 190 Bsp1286I G_dGCh'C Cuts at: 528 1252 1863 1956 2030 2220 2282 3080 528 Size: 724 611 93 74 190 62 798 1686 Fragments arranged by size: 1686 798 724 611 190 93 74 62 BspHI T'CATG_A Cuts at: 2670 2670 Size: 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1288 1502 1804 2185 1288 Size: 214 302 381 3341 Fragments arranged by size: 3341 381 302 214 BsrBI CCG'CTC Cuts at: 50 2530 2584 4068 4233 50 Size: 2480 54 1484 165 55 Fragments arranged by size: 2480 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 682 2150 682 Size: 1468 2770 BsrFI r'CCGG_y Cuts at: 2236 2417 2236 Size: 181 4057 BssSI C'ACGA_G Cuts at: 2509 3217 2509 Size: 708 3530 BstAPI GCAn_nnn'nTGC Cuts at: 1615 1615 Size: 4238 BstBI TT'CG_AA Cuts at: 2599 2599 Size: 4238 BstYI r'GATC_y Cuts at: 1 641 1263 1730 1753 2088 2334 2640 2652 Size: 640 622 467 23 335 246 306 12 Cuts at: 2652 2738 3790 1 Size: 86 1052 449 Fragments arranged by size: 1052 640 622 467 449 335 306 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2188 2369 2532 2188 Size: 181 163 3894 Fragments arranged by size: 3894 181 163 BtsI GCAGTG_nn' Cuts at: 1481 1481 Size: 4238 DraI TTT'AAA Cuts at: 1237 1237 Size: 4238 DrdI GACnn_nn'nnGTC Cuts at: 1944 3288 1944 Size: 1344 2894 EaeI y'GGCC_r Cuts at: 1106 1823 1997 2388 2415 3760 1106 Size: 717 174 391 27 1345 1584 Fragments arranged by size: 1584 1345 717 391 174 27 EagI C'GGCC_G Cuts at: 1823 1823 Size: 4238 EarI CTCTTCn'nnn_ Cuts at: 206 324 631 824 857 1070 2261 2471 206 Size: 118 307 193 33 213 1191 210 1973 Fragments arranged by size: 1973 1191 307 213 210 193 118 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 102 2446 3188 3334 4048 102 Size: 2344 742 146 714 292 Fragments arranged by size: 2344 742 714 292 146 EcoICRI GAG'CTC Cuts at: 1250 1250 Size: 4238 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 821 2063 2495 2848 3510 821 Size: 1242 432 353 662 1549 Fragments arranged by size: 1549 1242 662 432 353 EcoO109I rG'GnC_Cy Cuts at: 1377 1377 Size: 4238 EcoRI G'AATT_C Cuts at: 1242 1242 Size: 4238 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 660 2257 660 Size: 1597 2641 FspI TGC'GCA Cuts at: 2019 2019 Size: 4238 HaeII r_GCGC'y Cuts at: 1672 1920 3150 3921 1672 Size: 248 1230 771 1989 Fragments arranged by size: 1989 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2080 2110 2112 2142 2080 Size: 30 2 30 4176 Fragments arranged by size: 4176 30 30 2 HincII GTy'rAC Cuts at: 305 1277 305 Size: 972 3266 KasI G'GCGC_C Cuts at: 1668 1916 1668 Size: 248 3990 KpnI G_GTAC'C Cuts at: 1258 1258 Size: 4238 MfeI C'AATT_G Cuts at: 344 344 Size: 4238 MluI A'CGCG_T Cuts at: 3671 3671 Size: 4238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2997 3181 2997 Size: 184 4054 MscI TGG'CCA Cuts at: 1108 1999 1108 Size: 891 3347 MslI CAynn'nnrTG Cuts at: 2227 2354 2227 Size: 127 4111 MspA1I CmG'CkG Cuts at: 1349 1663 2023 2807 3052 1349 Size: 314 360 784 245 2535 Fragments arranged by size: 2535 784 360 314 245 NaeI GCC'GGC Cuts at: 2419 2419 Size: 4238 NarI GG'CG_CC Cuts at: 1669 1917 1669 Size: 248 3990 NgoMIV G'CCGG_C Cuts at: 2417 2417 Size: 4238 NsiI A_TGCA'T Cuts at: 662 662 Size: 4238 NspI r_CATG'y Cuts at: 1291 1791 3394 1291 Size: 500 1603 2135 Fragments arranged by size: 2135 1603 500 PacI TTA_AT'TAA Cuts at: 1046 1046 Size: 4238 PciI A'CATG_T Cuts at: 3390 3390 Size: 4238 PmeI GTTT'AAAC Cuts at: 1237 1237 Size: 4238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2684 2716 2684 Size: 32 4206 PstI C_TGCA'G Cuts at: 1285 1970 1285 Size: 685 3553 PvuI CG_AT'CG Cuts at: 104 104 Size: 4238 PvuII CAG'CTG Cuts at: 1663 2023 1663 Size: 360 3878 RsrII CG'GwC_CG Cuts at: 2433 2433 Size: 4238 SacI G_AGCT'C Cuts at: 1252 1252 Size: 4238 SalI G'TCGA_C Cuts at: 1275 1275 Size: 4238 SapI GCTCTTCn'nnn_ Cuts at: 2261 2471 2261 Size: 210 4028 SbfI CC_TGCA'GG Cuts at: 1285 1285 Size: 4238 ScaI AGT'ACT Cuts at: 3755 3755 Size: 4238 SfcI C'TryA_G Cuts at: 33 1067 1281 1966 2934 3125 33 Size: 1034 214 685 968 191 1146 Fragments arranged by size: 1146 1034 968 685 214 191 SfoI GGC'GCC Cuts at: 1670 1918 1670 Size: 248 3990 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4238 SmaI CCC'GGG Cuts at: 1260 1260 Size: 4238 SmlI C'TyrA_G Cuts at: 1393 2308 3022 3284 1393 Size: 915 714 262 2347 Fragments arranged by size: 2347 915 714 262 SpeI A'CTAG_T Cuts at: 67 630 67 Size: 563 3675 SphI G_CATG'C Cuts at: 1291 1791 1291 Size: 500 3738 StyI C'CwwG_G Cuts at: 1372 1726 1372 Size: 354 3884 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 166 1821 1954 2413 2622 166 Size: 1655 133 459 209 1782 Fragments arranged by size: 1782 1655 459 209 133 TatI w'GTAC_w Cuts at: 3753 3753 Size: 4238 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3755 3787 3755 Size: 32 4206 Tth111I GACn'n_nGTC Cuts at: 2035 2035 Size: 4238 XbaI T'CTAG_A Cuts at: 1269 1269 Size: 4238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1116 1733 1116 Size: 617 3621 XmaI C'CCGG_G Cuts at: 1258 1258 Size: 4238 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PacI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AleI ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI NotI NruI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI