(Circular) (Six-base) MAPSORT of: pF1KB9571.seq Check: 9363 from: 1 to: 4067 pF1KB9571 4067 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 372 372 Size: 4067 AccI GT'mk_AC Cuts at: 118 1105 118 Size: 987 3080 Acc65I G'GTAC_C Cuts at: 1083 1083 Size: 4067 AclI AA'CG_TT Cuts at: 3907 3907 Size: 4067 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 530 1892 2324 2677 3339 530 Size: 1362 432 353 662 1258 Fragments arranged by size: 1362 1258 662 432 353 AflII C'TTAA_G Cuts at: 847 847 Size: 4067 AflIII A'CryG_T Cuts at: 1419 3219 3500 1419 Size: 1800 281 1986 Fragments arranged by size: 1986 1800 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 491 953 491 Size: 462 3605 AlwNI CAG_nnn'CTG Cuts at: 577 1372 2810 577 Size: 795 1438 1834 Fragments arranged by size: 1834 1438 795 ApaLI G'TGCA_C Cuts at: 2905 2905 Size: 4067 ApoI r'AATT_y Cuts at: 890 1071 3420 3676 890 Size: 181 2349 256 1281 Fragments arranged by size: 2349 1281 256 181 AseI AT'TA_AT Cuts at: 20 199 20 Size: 179 3888 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4067 AvaI C'yCGr_G Cuts at: 863 1087 863 Size: 224 3843 BamHI G'GATC_C Cuts at: 1011 1092 1011 Size: 81 3986 BanI G'GyrC_C Cuts at: 550 929 1083 1497 1745 1780 550 Size: 379 154 414 248 35 2837 Fragments arranged by size: 2837 414 379 248 154 35 BanII G_rGCy'C Cuts at: 1081 2111 1081 Size: 1030 3037 BbeI G_GCGC'C Cuts at: 1501 1749 1501 Size: 248 3819 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 907 941 907 Size: 34 4033 BciVI GTATCCnnnnn_n' Cuts at: 1965 3021 4067 1965 Size: 1056 1046 1965 Fragments arranged by size: 1965 1056 1046 BclI T'GATC_A Cuts at: 590 1587 590 Size: 997 3070 BglI GCCn_nnn'nGGC Cuts at: 398 613 398 Size: 215 3852 BglII A'GATC_T Cuts at: 1582 3619 1582 Size: 2037 2030 BlpI GC'TnA_GC Cuts at: 1179 1179 Size: 4067 Bme1580I G_kGCm'C Cuts at: 1692 1785 2909 1692 Size: 93 1124 2850 Fragments arranged by size: 2850 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 1279 1360 1695 3839 1279 Size: 81 335 2144 1507 Fragments arranged by size: 2144 1507 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2434 2434 Size: 4067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 544 806 544 Size: 262 3805 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1243 2122 2836 3134 1243 Size: 879 714 298 2176 Fragments arranged by size: 2176 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 3771 3771 Size: 4067 BsaAI yAC'GTr Cuts at: 819 1420 819 Size: 601 3466 BsaBI GATnn'nnATC Cuts at: 6 358 418 595 1581 3618 6 Size: 352 60 177 986 2037 455 Fragments arranged by size: 2037 986 455 352 177 60 BsaHI Gr'CG_yC Cuts at: 1498 1746 2448 3944 1498 Size: 248 702 1496 1621 Fragments arranged by size: 1621 1496 702 248 BsaWI w'CCGG_w Cuts at: 1256 1777 2455 2866 3013 3287 1256 Size: 521 678 411 147 274 2036 Fragments arranged by size: 2036 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 713 743 713 Size: 30 4037 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 867 867 Size: 4067 BseYI C'CCAG_C Cuts at: 407 600 1492 2250 2915 3323 407 Size: 193 892 758 665 408 1151 Fragments arranged by size: 1151 892 758 665 408 193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 226 400 406 226 Size: 174 6 3887 Fragments arranged by size: 3887 174 6 BsiEI CG_ry'CG Cuts at: 104 1609 1655 2885 104 Size: 1505 46 1230 1286 Fragments arranged by size: 1505 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 624 1081 1859 2049 2909 624 Size: 457 778 190 860 1782 Fragments arranged by size: 1782 860 778 457 190 BsmI GAATG_Cn' Cuts at: 828 828 Size: 4067 Bsp1286I G_dGCh'C Cuts at: 624 1081 1692 1785 1859 2049 2111 2909 624 Size: 457 611 93 74 190 62 798 1782 Fragments arranged by size: 1782 798 611 457 190 93 74 62 BspHI T'CATG_A Cuts at: 731 2499 731 Size: 1768 2299 BspMI ACCTGCnnnn'nnnn_ Cuts at: 171 198 372 923 1117 1331 1633 2014 171 Size: 27 174 551 194 214 302 381 2224 Fragments arranged by size: 2224 551 381 302 214 194 174 27 BsrBI CCG'CTC Cuts at: 50 2359 2413 3897 4062 50 Size: 2309 54 1484 165 55 Fragments arranged by size: 2309 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 711 1043 1979 711 Size: 332 936 2799 Fragments arranged by size: 2799 936 332 BsrFI r'CCGG_y Cuts at: 2065 2246 2065 Size: 181 3886 BssSI C'ACGA_G Cuts at: 2338 3046 2338 Size: 708 3359 BstAPI GCAn_nnn'nTGC Cuts at: 505 1444 505 Size: 939 3128 BstBI TT'CG_AA Cuts at: 2428 2428 Size: 4067 BstYI r'GATC_y Cuts at: 1 353 359 1011 1092 1559 1582 1917 2163 Size: 352 6 652 81 467 23 335 246 Cuts at: 2163 2469 2481 2567 3619 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1052 652 467 449 352 335 306 246 86 81 23 12 6 BstZ17I GTA'TAC Cuts at: 119 119 Size: 4067 BtgI C'CryG_G Cuts at: 107 941 107 Size: 834 3233 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2017 2198 2361 2017 Size: 181 163 3723 Fragments arranged by size: 3723 181 163 BtsI GCAGTG_nn' Cuts at: 443 669 1310 443 Size: 226 641 3200 Fragments arranged by size: 3200 641 226 DraI TTT'AAA Cuts at: 1066 1066 Size: 4067 DrdI GACnn_nn'nnGTC Cuts at: 1773 3117 1773 Size: 1344 2723 EaeI y'GGCC_r Cuts at: 240 450 1652 1826 2217 2244 3589 240 Size: 210 1202 174 391 27 1345 718 Fragments arranged by size: 1345 1202 718 391 210 174 27 EagI C'GGCC_G Cuts at: 1652 1652 Size: 4067 EarI CTCTTCn'nnn_ Cuts at: 800 2090 2300 800 Size: 1290 210 2567 Fragments arranged by size: 2567 1290 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2275 3017 3163 3877 2275 Size: 742 146 714 2465 Fragments arranged by size: 2465 742 714 146 EcoICRI GAG'CTC Cuts at: 1079 1079 Size: 4067 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 530 544 806 1892 2324 2677 3339 530 Size: 14 262 1086 432 353 662 1258 Fragments arranged by size: 1258 1086 662 432 353 262 14 EcoNI CCTnn'n_nnAGG Cuts at: 581 581 Size: 4067 EcoO109I rG'GnC_Cy Cuts at: 1206 1206 Size: 4067 EcoRI G'AATT_C Cuts at: 1071 1071 Size: 4067 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2086 2086 Size: 4067 FspI TGC'GCA Cuts at: 1848 1848 Size: 4067 HaeII r_GCGC'y Cuts at: 1501 1749 2979 3750 1501 Size: 248 1230 771 1818 Fragments arranged by size: 1818 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 713 745 1909 1939 1941 1971 713 Size: 32 1164 30 2 30 2809 Fragments arranged by size: 2809 1164 32 30 30 2 HincII GTy'rAC Cuts at: 1106 1106 Size: 4067 KasI G'GCGC_C Cuts at: 1497 1745 1497 Size: 248 3819 KpnI G_GTAC'C Cuts at: 1087 1087 Size: 4067 MluI A'CGCG_T Cuts at: 3500 3500 Size: 4067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2826 3010 2826 Size: 184 3883 MscI TGG'CCA Cuts at: 242 452 1828 242 Size: 210 1376 2481 Fragments arranged by size: 2481 1376 210 MslI CAynn'nnrTG Cuts at: 935 1054 2056 2183 935 Size: 119 1002 127 2819 Fragments arranged by size: 2819 1002 127 119 MspA1I CmG'CkG Cuts at: 1178 1492 1852 2636 2881 1178 Size: 314 360 784 245 2364 Fragments arranged by size: 2364 784 360 314 245 NaeI GCC'GGC Cuts at: 2248 2248 Size: 4067 NarI GG'CG_CC Cuts at: 1498 1746 1498 Size: 248 3819 NcoI C'CATG_G Cuts at: 107 941 107 Size: 834 3233 NdeI CA'TA_TG Cuts at: 1004 1004 Size: 4067 NgoMIV G'CCGG_C Cuts at: 2246 2246 Size: 4067 NspI r_CATG'y Cuts at: 1120 1620 3223 1120 Size: 500 1603 1964 Fragments arranged by size: 1964 1603 500 PciI A'CATG_T Cuts at: 3219 3219 Size: 4067 PmeI GTTT'AAAC Cuts at: 1066 1066 Size: 4067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2513 2545 2513 Size: 32 4035 PshAI GACnn'nnGTC Cuts at: 439 523 439 Size: 84 3983 PstI C_TGCA'G Cuts at: 1114 1799 1114 Size: 685 3382 PvuI CG_AT'CG Cuts at: 104 104 Size: 4067 PvuII CAG'CTG Cuts at: 1492 1852 1492 Size: 360 3707 RsrII CG'GwC_CG Cuts at: 2262 2262 Size: 4067 SacI G_AGCT'C Cuts at: 1081 1081 Size: 4067 SalI G'TCGA_C Cuts at: 1104 1104 Size: 4067 SapI GCTCTTCn'nnn_ Cuts at: 800 2090 2300 800 Size: 1290 210 2567 Fragments arranged by size: 2567 1290 210 SbfI CC_TGCA'GG Cuts at: 1114 1114 Size: 4067 ScaI AGT'ACT Cuts at: 3584 3584 Size: 4067 SfcI C'TryA_G Cuts at: 33 1110 1795 2763 2954 33 Size: 1077 685 968 191 1146 Fragments arranged by size: 1146 1077 968 685 191 SfiI GGCCn_nnn'nGGCC Cuts at: 398 398 Size: 4067 SfoI GGC'GCC Cuts at: 1499 1747 1499 Size: 248 3819 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4067 SmaI CCC'GGG Cuts at: 1089 1089 Size: 4067 SmlI C'TyrA_G Cuts at: 847 1222 2137 2851 3113 847 Size: 375 915 714 262 1801 Fragments arranged by size: 1801 915 714 375 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4067 SphI G_CATG'C Cuts at: 1120 1620 1120 Size: 500 3567 StyI C'CwwG_G Cuts at: 107 186 941 1201 1555 107 Size: 79 755 260 354 2619 Fragments arranged by size: 2619 755 354 260 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1650 1783 2242 2451 1650 Size: 133 459 209 3266 Fragments arranged by size: 3266 459 209 133 TatI w'GTAC_w Cuts at: 3582 3582 Size: 4067 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 362 394 479 511 942 974 1034 1066 3584 Size: 32 85 32 431 32 60 32 2518 Cuts at: 3584 3616 362 Size: 32 813 Fragments arranged by size: 2518 813 431 85 60 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 278 1864 278 Size: 1586 2481 XbaI T'CTAG_A Cuts at: 1098 1098 Size: 4067 XcmI CCAnnnn_n'nnnnTGG Cuts at: 265 364 1562 265 Size: 99 1198 2770 Fragments arranged by size: 2770 1198 99 XmaI C'CCGG_G Cuts at: 1087 1087 Size: 4067 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AgeI AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI