Restriction map for
pF1KB9560
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI Alw44I ApaI ApaLI AscI
AspI AvrII BbeI BbrPI BbvCI
BcgI BciVI BglI BglII BlnI
BlpI Bpu1102I BsaHI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraIII
DrdI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HgaI
Hin1I KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SwaI Tth111I Van91I XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflII 667 CTTAAG C^TTAAG
AgeI 173 ACCGGT A^CCGGT
AhdI 3055 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2094 CCTGAGG CC^T[ACGT]AGG
AseI 1210 ATTAAT AT^TAAT
AsnI 1210 ATTAAT AT^TAAT
Asp700I 2574 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3055 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2223 TGCGCA TGC^GCA
BamHI 1532 GGATCC G^GATCC
BbsI 2089 GAAGAC GAAGAC(2/6)
BfrI 667 CTTAAG C^TTAAG
BpmI 2110 CTGGAG CTGGAG(16/14)
BpuAI 2089 GAAGAC GAAGAC(2/6)
BsaAI 2558 TACGTA [CT]AC^GT[GA]
BsaBI 2052 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 173 ACCGGT [AT]^CCGG[AT]
BspHI 795 TCATGA T^CATGA
BspLU11I 3364 ACATGT A^CATGT
Bsu36I 2094 CCTGAGG CC^T[ACGT]AGG
DraI 526 TTTAAA TTT^AAA
Eam1105I 3055 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2094 CCTGAGG CC^T[ACGT]AGG
FspI 2223 TGCGCA TGC^GCA
HpaI 163 GTTAAC GTT^AAC
MamI 2052 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1737 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 869 CTCGAG C^TCGAG
PinAI 173 ACCGGT A^CCGGT
PshBI 1210 ATTAAT AT^TAAT
RcaI 795 TCATGA T^CATGA
SexAI 1562 ACCAGGT A^CC[AT]GGT
SnaBI 2558 TACGTA TAC^GTA
SphI 2514 GCATGC GCATG^C
SspI 3268 AATATT AAT^ATT
XhoI 869 CTCGAG C^TCGAG
XmnI 2574 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1850 GGTACC G^GTACC
2237 GGTACC
AflIII 1505 ACACGT A^C[GA][CT]GT
3364 ACATGT
AlwNI 1406 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
2519 CAGTTGCTG
Aor51HI 110 AGCGCT AGC^GCT
2330 AGCGCT
Asp718I 1850 GGTACC G^GTACC
2237 GGTACC
AvaI 578 CTCGGG C^[CT]CG[GA]G
869 CTCGAG
BclI 945 TGATCA T^GATCA
3201 TGATCA
BcnI 438 CCCGG CC^[GC]GG
2283 CCGGG
BsmI 765 GAATGC GAATGC(1/-1)
967 GCATTC
BsoBI 578 CTCGGG C^[CT]CG[GA]G
869 CTCGAG
BspMI 689 GCAGGT ACCTGC(4/8)
1073 GCAGGT
BsrDI 2355 CATTGC GCAATG(2/0)
2964 CATTGC
BsrFI 173 ACCGGT [GA]^CCGG[CT]
937 ACCGGC
BstXI 3057 CCAAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3342 CCAGAAAAATGG
Cfr10I 173 ACCGGT [GA]^CCGG[CT]
937 ACCGGC
DraII 1994 AGGTCCT [GA]G^G[ACGT]CC[CT]
2374 GGGACCC
DsaI 1730 CCGTGG C^C[GA][CT]GG
2021 CCGTGG
Eco47III 110 AGCGCT AGC^GCT
2330 AGCGCT
EcoO109I 1994 AGGTCCT [GA]G^G[ACGT]CC[CT]
2374 GGGACCC
EcoT14I 2889 CCAAGG C^C[AT][AT]GG
3104 CCAAGG
FauI 1675 CCCGC CCCGC(4/6)
2310 GCGGG
FbaI 945 TGATCA T^GATCA
3201 TGATCA
HaeII 110 AGCGCT [GA]GCGC^[CT]
2330 AGCGCT
HincII 163 GTTAAC GT[CT]^[GA]AC
1579 GTTGAC
HindII 163 GTTAAC GT[CT]^[GA]AC
1579 GTTGAC
KpnI 1850 GGTACC GGTAC^C
2237 GGTACC
NciI 438 CCCGG CC^[GC]GG
2283 CCGGG
PpuMI 1994 AGGTCCT [GA]G^G[AT]CC[CT]
2374 GGGACCC
SacI 652 GAGCTC GAGCT^C
3117 GAGCTC
SapI 649 GAAGAGC GCTCTTC(1/4)
1264 GAAGAGC
StuI 1696 AGGCCT AGG^CCT
1907 AGGCCT
StyI 2889 CCAAGG C^C[AT][AT]GG
3104 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanI
BanII BbvI BfaI BmyI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII