(Circular) (Six-base) MAPSORT of: pF1KB9560.seq Check: 1215 from: 1 to: 6521 pF1KB9560 6521 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AccI GT'mk_AC Cuts at: 3559 3559 Size: 6521 Acc65I G'GTAC_C Cuts at: 1958 2345 3537 1958 Size: 387 1192 4942 Fragments arranged by size: 4942 1192 387 AclI AA'CG_TT Cuts at: 6361 6361 Size: 6521 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 776 1620 1992 2446 2533 2673 2680 2713 3098 Size: 844 372 454 87 140 7 33 385 Cuts at: 3098 3285 4346 4778 5131 5793 776 Size: 187 1061 432 353 662 1504 Fragments arranged by size: 1504 1061 844 662 454 432 385 372 353 187 140 87 33 7 AfeI AGC'GCT Cuts at: 220 2440 220 Size: 2220 4301 AflII C'TTAA_G Cuts at: 775 775 Size: 6521 AflIII A'CryG_T Cuts at: 1613 3472 3873 5673 5954 1613 Size: 1859 401 1800 281 2180 Fragments arranged by size: 2180 1859 1800 401 281 AgeI A'CCGG_T Cuts at: 281 281 Size: 6521 AhdI GACnn_n'nnGTC Cuts at: 3168 3168 Size: 6521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2645 2645 Size: 6521 AlwNI CAG_nnn'CTG Cuts at: 1519 2632 3826 5264 1519 Size: 1113 1194 1438 2776 Fragments arranged by size: 2776 1438 1194 1113 ApaLI G'TGCA_C Cuts at: 5359 5359 Size: 6521 ApoI r'AATT_y Cuts at: 333 625 855 1031 2123 2411 2683 3525 5874 Size: 292 230 176 1092 288 272 842 2349 Cuts at: 5874 6130 333 Size: 256 724 Fragments arranged by size: 2349 1092 842 724 292 288 272 256 230 176 AseI AT'TA_AT Cuts at: 20 1319 20 Size: 1299 5222 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6521 AvaI C'yCGr_G Cuts at: 686 977 3541 686 Size: 291 2564 3666 Fragments arranged by size: 3666 2564 291 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3200 3233 3200 Size: 33 6488 BamHI G'GATC_C Cuts at: 1640 3546 1640 Size: 1906 4615 BanI G'GyrC_C Cuts at: 748 1958 2345 3537 3951 4199 4234 748 Size: 1210 387 1192 414 248 35 3035 Fragments arranged by size: 3035 1210 1192 414 387 248 35 BanII G_rGCy'C Cuts at: 352 764 3229 3271 3535 4565 352 Size: 412 2465 42 264 1030 2308 Fragments arranged by size: 2465 2308 1030 412 264 42 BbeI G_GCGC'C Cuts at: 3955 4203 3955 Size: 248 6273 BbsI GAAGACnn'nnnn_ Cuts at: 2204 2204 Size: 6521 BciVI GTATCCnnnnn_n' Cuts at: 4419 5475 6521 4419 Size: 1056 1046 4419 Fragments arranged by size: 4419 1056 1046 BclI T'GATC_A Cuts at: 1053 3309 4041 1053 Size: 2256 732 3533 Fragments arranged by size: 3533 2256 732 BglII A'GATC_T Cuts at: 4036 6073 4036 Size: 2037 4484 BlpI GC'TnA_GC Cuts at: 3633 3633 Size: 6521 Bme1580I G_kGCm'C Cuts at: 4146 4239 5363 4146 Size: 93 1124 5304 Fragments arranged by size: 5304 1124 93 BmgBI CAC'GTC Cuts at: 1616 1616 Size: 6521 BmrI ACTGGGnnnn_n' Cuts at: 437 958 3733 3814 4149 6293 437 Size: 521 2775 81 335 2144 665 Fragments arranged by size: 2775 2144 665 521 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3137 3313 4888 3137 Size: 176 1575 4770 Fragments arranged by size: 4770 1575 176 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 2239 2239 Size: 6521 Bpu10I CC'TnA_GC Cuts at: 1136 1136 Size: 6521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 196 802 2793 3697 4576 5290 5588 196 Size: 606 1991 904 879 714 298 1129 Fragments arranged by size: 1991 1129 904 879 714 606 298 BsaI GGTCTCn'nnnn_ Cuts at: 187 1519 1709 6225 187 Size: 1332 190 4516 483 Fragments arranged by size: 4516 1332 483 190 BsaAI yAC'GTr Cuts at: 2668 3874 2668 Size: 1206 5315 BsaBI GATnn'nnATC Cuts at: 6 2164 4035 6072 6 Size: 2158 1871 2037 455 Fragments arranged by size: 2158 2037 1871 455 BsaHI Gr'CG_yC Cuts at: 3952 4200 4902 6398 3952 Size: 248 702 1496 4075 Fragments arranged by size: 4075 1496 702 248 BsaWI w'CCGG_w Cuts at: 281 3710 4231 4909 5320 5467 5741 281 Size: 3429 521 678 411 147 274 1061 Fragments arranged by size: 3429 1061 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 494 524 494 Size: 30 6491 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 205 585 668 754 1396 2533 2620 205 Size: 380 83 86 642 1137 87 4106 Fragments arranged by size: 4106 1137 642 380 87 86 83 BseYI C'CCAG_C Cuts at: 3946 4704 5369 5777 3946 Size: 758 665 408 4690 Fragments arranged by size: 4690 758 665 408 BsiEI CG_ry'CG Cuts at: 104 4063 4109 5339 104 Size: 3959 46 1230 1286 Fragments arranged by size: 3959 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 764 1597 3229 3535 4313 4503 5363 764 Size: 833 1632 306 778 190 860 1922 Fragments arranged by size: 1922 1632 860 833 778 306 190 BsmI GAATG_Cn' Cuts at: 879 1075 879 Size: 196 6325 BsmBI CGTCTCn'nnnn_ Cuts at: 1622 2499 3499 1622 Size: 877 1000 4644 Fragments arranged by size: 4644 1000 877 Bsp1286I G_dGCh'C Cuts at: 352 764 1597 3229 3271 3535 4146 4239 4313 Size: 412 833 1632 42 264 611 93 74 Cuts at: 4313 4503 4565 5363 352 Size: 190 62 798 1510 Fragments arranged by size: 1632 1510 833 798 611 412 264 190 93 74 62 42 BspHI T'CATG_A Cuts at: 903 4953 903 Size: 4050 2471 BspMI ACCTGCnnnn'nnnn_ Cuts at: 788 1172 3571 3785 4087 4468 788 Size: 384 2399 214 302 381 2841 Fragments arranged by size: 2841 2399 384 381 302 214 BsrBI CCG'CTC Cuts at: 50 4813 4867 6351 6516 50 Size: 4763 54 1484 165 55 Fragments arranged by size: 4763 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2462 3071 4433 2462 Size: 609 1362 4550 Fragments arranged by size: 4550 1362 609 BsrFI r'CCGG_y Cuts at: 281 1045 4519 4700 281 Size: 764 3474 181 2102 Fragments arranged by size: 3474 2102 764 181 BssSI C'ACGA_G Cuts at: 4792 5500 4792 Size: 708 5813 BstAPI GCAn_nnn'nTGC Cuts at: 3898 3898 Size: 6521 BstBI TT'CG_AA Cuts at: 4882 4882 Size: 6521 BstXI CCAn_nnnn'nTGG Cuts at: 3172 3457 3172 Size: 285 6236 BstYI r'GATC_y Cuts at: 1 385 1640 2569 3546 4013 4036 4371 4617 Size: 384 1255 929 977 467 23 335 246 Cuts at: 4617 4923 4935 5021 6073 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1255 1052 977 929 467 449 384 335 306 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 2203 2203 Size: 6521 BtgI C'CryG_G Cuts at: 107 1838 2129 107 Size: 1731 291 4499 Fragments arranged by size: 4499 1731 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4471 4652 4815 4471 Size: 181 163 6177 Fragments arranged by size: 6177 181 163 BtsI GCAGTG_nn' Cuts at: 944 3312 3764 944 Size: 2368 452 3701 Fragments arranged by size: 3701 2368 452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1714 1749 1714 Size: 35 6486 DraI TTT'AAA Cuts at: 636 3520 636 Size: 2884 3637 DrdI GACnn_nn'nnGTC Cuts at: 4227 5571 4227 Size: 1344 5177 EaeI y'GGCC_r Cuts at: 110 824 1478 1841 2228 3326 4106 4280 4671 Size: 714 654 363 387 1098 780 174 391 Cuts at: 4671 4698 6043 110 Size: 27 1345 588 Fragments arranged by size: 1345 1098 780 714 654 588 391 387 363 174 27 EagI C'GGCC_G Cuts at: 4106 4106 Size: 6521 EarI CTCTTCn'nnn_ Cuts at: 752 1367 1487 2918 2953 3106 3304 4544 4754 Size: 615 120 1431 35 153 198 1240 210 Cuts at: 4754 752 Size: 2519 Fragments arranged by size: 2519 1431 1240 615 210 198 153 120 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2355 4729 5471 5617 6331 2355 Size: 2374 742 146 714 2545 Fragments arranged by size: 2545 2374 742 714 146 EcoICRI GAG'CTC Cuts at: 762 3227 3533 762 Size: 2465 306 3750 Fragments arranged by size: 3750 2465 306 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 776 1620 1992 2239 2446 2533 2673 2680 2713 Size: 844 372 247 207 87 140 7 33 Cuts at: 2713 3098 3285 4346 4778 5131 5793 776 Size: 385 187 1061 432 353 662 1504 Fragments arranged by size: 1504 1061 844 662 432 385 372 353 247 207 187 140 87 33 7 EcoNI CCTnn'n_nnAGG Cuts at: 1814 2319 3262 1814 Size: 505 943 5073 Fragments arranged by size: 5073 943 505 EcoO109I rG'GnC_Cy Cuts at: 2103 2483 3660 2103 Size: 380 1177 4964 Fragments arranged by size: 4964 1177 380 EcoRI G'AATT_C Cuts at: 3525 3525 Size: 6521 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 237 547 4540 237 Size: 310 3993 2218 Fragments arranged by size: 3993 2218 310 FspI TGC'GCA Cuts at: 2333 4302 2333 Size: 1969 4552 HaeII r_GCGC'y Cuts at: 222 2442 3955 4203 5433 6204 222 Size: 2220 1513 248 1230 771 539 Fragments arranged by size: 2220 1513 1230 771 539 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 580 612 3154 3186 4363 4393 4395 4425 580 Size: 32 2542 32 1177 30 2 30 2676 Fragments arranged by size: 2676 2542 1177 32 32 30 30 2 HincII GTy'rAC Cuts at: 273 1689 3560 273 Size: 1416 1871 3234 Fragments arranged by size: 3234 1871 1416 HindIII A'AGCT_T Cuts at: 463 557 778 463 Size: 94 221 6206 Fragments arranged by size: 6206 221 94 HpaI GTT'AAC Cuts at: 273 273 Size: 6521 KasI G'GCGC_C Cuts at: 3951 4199 3951 Size: 248 6273 KpnI G_GTAC'C Cuts at: 1962 2349 3541 1962 Size: 387 1192 4942 Fragments arranged by size: 4942 1192 387 MluI A'CGCG_T Cuts at: 5954 5954 Size: 6521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 566 2377 5280 5464 566 Size: 1811 2903 184 1623 Fragments arranged by size: 2903 1811 1623 184 MscI TGG'CCA Cuts at: 112 1480 1843 2230 3328 4282 112 Size: 1368 363 387 1098 954 2351 Fragments arranged by size: 2351 1368 1098 954 387 363 MslI CAynn'nnrTG Cuts at: 1849 4510 4637 1849 Size: 2661 127 3733 Fragments arranged by size: 3733 2661 127 MspA1I CmG'CkG Cuts at: 487 738 1631 3632 3946 4306 5090 5335 487 Size: 251 893 2001 314 360 784 245 1673 Fragments arranged by size: 2001 1673 893 784 360 314 251 245 NaeI GCC'GGC Cuts at: 4702 4702 Size: 6521 NarI GG'CG_CC Cuts at: 3952 4200 3952 Size: 248 6273 NcoI C'CATG_G Cuts at: 107 107 Size: 6521 NgoMIV G'CCGG_C Cuts at: 4700 4700 Size: 6521 NspI r_CATG'y Cuts at: 2539 2626 3476 3574 4074 5677 2539 Size: 87 850 98 500 1603 3383 Fragments arranged by size: 3383 1603 850 500 98 87 PciI A'CATG_T Cuts at: 3472 5673 3472 Size: 2201 4320 PfoI T'CCnGG_A Cuts at: 551 551 Size: 6521 PmeI GTTT'AAAC Cuts at: 3520 3520 Size: 6521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4967 4999 4967 Size: 32 6489 PpuMI rG'GwC_Cy Cuts at: 2103 2483 2103 Size: 380 6141 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 518 550 518 Size: 32 6489 PstI C_TGCA'G Cuts at: 3568 4253 3568 Size: 685 5836 PvuI CG_AT'CG Cuts at: 104 104 Size: 6521 PvuII CAG'CTG Cuts at: 487 738 1631 3946 4306 487 Size: 251 893 2315 360 2702 Fragments arranged by size: 2702 2315 893 360 251 RsrII CG'GwC_CG Cuts at: 4716 4716 Size: 6521 SacI G_AGCT'C Cuts at: 764 3229 3535 764 Size: 2465 306 3750 Fragments arranged by size: 3750 2465 306 SalI G'TCGA_C Cuts at: 3558 3558 Size: 6521 SanDI GG'GwC_CC Cuts at: 2483 2483 Size: 6521 SapI GCTCTTCn'nnn_ Cuts at: 752 1367 4544 4754 752 Size: 615 3177 210 2519 Fragments arranged by size: 3177 2519 615 210 SbfI CC_TGCA'GG Cuts at: 3568 3568 Size: 6521 ScaI AGT'ACT Cuts at: 6038 6038 Size: 6521 SexAI A'CCwGG_T Cuts at: 1670 1670 Size: 6521 SfcI C'TryA_G Cuts at: 33 1176 1811 1861 3564 4249 5217 5408 33 Size: 1143 635 50 1703 685 968 191 1146 Fragments arranged by size: 1703 1146 1143 968 685 635 191 50 SfoI GGC'GCC Cuts at: 3953 4201 3953 Size: 248 6273 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6521 SmaI CCC'GGG Cuts at: 3543 3543 Size: 6521 SmlI C'TyrA_G Cuts at: 211 775 781 977 2808 3676 4591 5305 5567 Size: 564 6 196 1831 868 915 714 262 Cuts at: 5567 211 Size: 1165 Fragments arranged by size: 1831 1165 915 868 714 564 262 196 6 SnaBI TAC'GTA Cuts at: 2668 2668 Size: 6521 SpeI A'CTAG_T Cuts at: 67 67 Size: 6521 SphI G_CATG'C Cuts at: 2626 3574 4074 2626 Size: 948 500 5073 Fragments arranged by size: 5073 948 500 SspI AAT'ATT Cuts at: 3378 3378 Size: 6521 StuI AGG'CCT Cuts at: 1806 2017 1806 Size: 211 6310 StyI C'CwwG_G Cuts at: 107 2997 3212 3655 4009 107 Size: 2890 215 443 354 2619 Fragments arranged by size: 2890 2619 443 354 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1246 1576 4104 4237 4696 4905 1246 Size: 330 2528 133 459 209 2862 Fragments arranged by size: 2862 2528 459 330 209 133 TatI w'GTAC_w Cuts at: 3115 6036 3115 Size: 2921 3600 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1480 1512 6038 6070 1480 Size: 32 4526 32 1931 Fragments arranged by size: 4526 1931 32 32 Tth111I GACn'n_nGTC Cuts at: 4318 4318 Size: 6521 XbaI T'CTAG_A Cuts at: 598 990 1065 3552 598 Size: 392 75 2487 3567 Fragments arranged by size: 3567 2487 392 75 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 549 885 1651 2094 4016 114 Size: 435 336 766 443 1922 2619 Fragments arranged by size: 2619 1922 766 443 435 336 XhoI C'TCGA_G Cuts at: 977 977 Size: 6521 XmaI C'CCGG_G Cuts at: 3541 3541 Size: 6521 XmnI GAAnn'nnTTC Cuts at: 2686 2686 Size: 6521 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AleI AloI ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmtI BsgI BsiWI BspEI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI SacII SfiI SgrAI SrfI SwaI ZraI