(Circular) (Six-base) MAPSORT of: pF1KB9559.seq Check: 6707 from: 1 to: 6371 pF1KB9559 6371 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AatII G_ACGT'C Cuts at: 1929 1929 Size: 6371 AccI GT'mk_AC Cuts at: 2052 2322 3409 2052 Size: 270 1087 5014 Fragments arranged by size: 5014 1087 270 Acc65I G'GTAC_C Cuts at: 2365 3387 2365 Size: 1022 5349 AclI AA'CG_TT Cuts at: 1080 6211 1080 Size: 5131 1240 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 580 2731 4196 4628 4981 5643 580 Size: 2151 1465 432 353 662 1308 Fragments arranged by size: 2151 1465 1308 662 432 353 AfeI AGC'GCT Cuts at: 1492 1492 Size: 6371 AflII C'TTAA_G Cuts at: 153 153 Size: 6371 AflIII A'CryG_T Cuts at: 526 1844 3041 3723 5523 5804 526 Size: 1318 1197 682 1800 281 1093 Fragments arranged by size: 1800 1318 1197 1093 682 281 AgeI A'CCGG_T Cuts at: 289 289 Size: 6371 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2021 2053 2633 2665 2735 2767 2021 Size: 32 580 32 70 32 5625 Fragments arranged by size: 5625 580 70 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 967 2817 2931 3676 5114 967 Size: 1850 114 745 1438 2224 Fragments arranged by size: 2224 1850 1438 745 114 ApaI G_GGCC'C Cuts at: 1549 1549 Size: 6371 ApaLI G'TGCA_C Cuts at: 5209 5209 Size: 6371 ApoI r'AATT_y Cuts at: 458 1177 2128 2264 3018 3136 3187 3375 5724 Size: 719 951 136 754 118 51 188 2349 Cuts at: 5724 5980 458 Size: 256 849 Fragments arranged by size: 2349 951 849 754 719 256 188 136 118 51 AseI AT'TA_AT Cuts at: 20 191 833 20 Size: 171 642 5558 Fragments arranged by size: 5558 642 171 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6371 AvaI C'yCGr_G Cuts at: 1765 3127 3391 1765 Size: 1362 264 4745 Fragments arranged by size: 4745 1362 264 BamHI G'GATC_C Cuts at: 510 3396 510 Size: 2886 3485 BanI G'GyrC_C Cuts at: 2365 2524 3387 3801 4049 4084 2365 Size: 159 863 414 248 35 4652 Fragments arranged by size: 4652 863 414 248 159 35 BanII G_rGCy'C Cuts at: 799 1549 3385 4415 799 Size: 750 1836 1030 2755 Fragments arranged by size: 2755 1836 1030 750 BbeI G_GCGC'C Cuts at: 3805 4053 3805 Size: 248 6123 BbsI GAAGACnn'nnnn_ Cuts at: 134 523 1961 3044 134 Size: 389 1438 1083 3461 Fragments arranged by size: 3461 1438 1083 389 BbvCI CC'TCA_GC Cuts at: 1676 2978 1676 Size: 1302 5069 BciVI GTATCCnnnnn_n' Cuts at: 3114 4269 5325 6371 3114 Size: 1155 1056 1046 3114 Fragments arranged by size: 3114 1155 1056 1046 BclI T'GATC_A Cuts at: 2376 3891 2376 Size: 1515 4856 BfrBI ATG'CAT Cuts at: 449 1920 449 Size: 1471 4900 BglI GCCn_nnn'nGGC Cuts at: 1749 1749 Size: 6371 BglII A'GATC_T Cuts at: 3153 3886 5923 3153 Size: 733 2037 3601 Fragments arranged by size: 3601 2037 733 BlpI GC'TnA_GC Cuts at: 3483 3483 Size: 6371 Bme1580I G_kGCm'C Cuts at: 1549 3996 4089 5213 1549 Size: 2447 93 1124 2707 Fragments arranged by size: 2707 2447 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 2218 3583 3664 3999 6143 2218 Size: 1365 81 335 2144 2446 Fragments arranged by size: 2446 2144 1365 335 81 BmtI G_CTAG'C Cuts at: 2303 2303 Size: 6371 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 478 4738 478 Size: 4260 2111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 448 640 1250 2090 2953 448 Size: 192 610 840 863 3866 Fragments arranged by size: 3866 863 840 610 192 Bpu10I CC'TnA_GC Cuts at: 1232 1676 2978 1232 Size: 444 1302 4625 Fragments arranged by size: 4625 1302 444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1107 3547 4426 5140 5438 1107 Size: 2440 879 714 298 2040 Fragments arranged by size: 2440 2040 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 298 687 6075 298 Size: 389 5388 594 Fragments arranged by size: 5388 594 389 BsaAI yAC'GTr Cuts at: 3724 3724 Size: 6371 BsaBI GATnn'nnATC Cuts at: 6 3885 5922 6 Size: 3879 2037 455 Fragments arranged by size: 3879 2037 455 BsaHI Gr'CG_yC Cuts at: 1926 2868 3802 4050 4752 6248 1926 Size: 942 934 248 702 1496 2049 Fragments arranged by size: 2049 1496 942 934 702 248 BsaWI w'CCGG_w Cuts at: 289 3560 4081 4759 5170 5317 5591 289 Size: 3271 521 678 411 147 274 1069 Fragments arranged by size: 3271 1069 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 605 635 1626 1656 2021 2051 2063 2093 605 Size: 30 991 30 365 30 12 30 4883 Fragments arranged by size: 4883 991 365 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1634 2042 3248 1634 Size: 408 1206 4757 Fragments arranged by size: 4757 1206 408 BseYI C'CCAG_C Cuts at: 361 1707 2316 2962 3796 4554 5219 5627 361 Size: 1346 609 646 834 758 665 408 1105 Fragments arranged by size: 1346 1105 834 758 665 646 609 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 2521 2459 Size: 62 6309 BsiEI CG_ry'CG Cuts at: 104 3913 3959 5189 104 Size: 3809 46 1230 1286 Fragments arranged by size: 3809 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 434 799 928 2202 3385 4163 4353 5213 434 Size: 365 129 1274 1183 778 190 860 1592 Fragments arranged by size: 1592 1274 1183 860 778 365 190 129 BsmI GAATG_Cn' Cuts at: 1787 1890 1787 Size: 103 6268 BsmBI CGTCTCn'nnnn_ Cuts at: 138 138 Size: 6371 Bsp1286I G_dGCh'C Cuts at: 434 799 928 1549 2202 3385 3996 4089 4163 Size: 365 129 621 653 1183 611 93 74 Cuts at: 4163 4353 4415 5213 434 Size: 190 62 798 1592 Fragments arranged by size: 1592 1183 798 653 621 611 365 190 129 93 74 62 BspHI T'CATG_A Cuts at: 4803 4803 Size: 6371 BspMI ACCTGCnnnn'nnnn_ Cuts at: 3421 3635 3937 4318 3421 Size: 214 302 381 5474 Fragments arranged by size: 5474 381 302 214 BsrBI CCG'CTC Cuts at: 50 4663 4717 6201 6366 50 Size: 4613 54 1484 165 55 Fragments arranged by size: 4613 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 262 4283 262 Size: 4021 2350 BsrFI r'CCGG_y Cuts at: 289 1973 4369 4550 289 Size: 1684 2396 181 2110 Fragments arranged by size: 2396 2110 1684 181 BssSI C'ACGA_G Cuts at: 4642 5350 4642 Size: 708 5663 BstAPI GCAn_nnn'nTGC Cuts at: 3748 3748 Size: 6371 BstBI TT'CG_AA Cuts at: 4732 4732 Size: 6371 BstEII G'GTnAC_C Cuts at: 319 319 Size: 6371 BstXI CCAn_nnnn'nTGG Cuts at: 2316 3114 2316 Size: 798 5573 BstYI r'GATC_y Cuts at: 1 335 510 888 1116 1254 2750 3153 3396 Size: 334 175 378 228 138 1496 403 243 Cuts at: 3396 3863 3886 4221 4467 4773 4785 4871 5923 Size: 467 23 335 246 306 12 86 1052 Cuts at: 5923 1 Size: 449 Fragments arranged by size: 1496 1052 467 449 403 378 335 334 306 246 243 228 175 138 86 23 12 BstZ17I GTA'TAC Cuts at: 2323 2323 Size: 6371 Bsu36I CC'TnA_GG Cuts at: 648 648 Size: 6371 BtgI C'CryG_G Cuts at: 107 2732 2754 107 Size: 2625 22 3724 Fragments arranged by size: 3724 2625 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1419 4321 4502 4665 1419 Size: 2902 181 163 3125 Fragments arranged by size: 3125 2902 181 163 BtsI GCAGTG_nn' Cuts at: 614 3614 614 Size: 3000 3371 ClaI AT'CG_AT Cuts at: 2876 2876 Size: 6371 DraI TTT'AAA Cuts at: 1077 3370 1077 Size: 2293 4078 DraIII CAC_nnn'GTG Cuts at: 2800 2800 Size: 6371 DrdI GACnn_nn'nnGTC Cuts at: 742 3166 4077 5421 742 Size: 2424 911 1344 1692 Fragments arranged by size: 2424 1692 1344 911 EaeI y'GGCC_r Cuts at: 1389 3956 4130 4521 4548 5893 1389 Size: 2567 174 391 27 1345 1867 Fragments arranged by size: 2567 1867 1345 391 174 27 EagI C'GGCC_G Cuts at: 3956 3956 Size: 6371 EarI CTCTTCn'nnn_ Cuts at: 2111 2394 4394 4604 2111 Size: 283 2000 210 3878 Fragments arranged by size: 3878 2000 283 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2774 4579 5321 5467 6181 2774 Size: 1805 742 146 714 2964 Fragments arranged by size: 2964 1805 742 714 146 EcoICRI GAG'CTC Cuts at: 797 3383 797 Size: 2586 3785 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 448 580 640 1250 2090 2731 2953 4196 4628 Size: 132 60 610 840 641 222 1243 432 Cuts at: 4628 4981 5643 448 Size: 353 662 1176 Fragments arranged by size: 1243 1176 840 662 641 610 432 353 222 132 60 EcoO109I rG'GnC_Cy Cuts at: 365 416 1545 2341 3510 365 Size: 51 1129 796 1169 3226 Fragments arranged by size: 3226 1169 1129 796 51 EcoRI G'AATT_C Cuts at: 458 3375 458 Size: 2917 3454 EcoRV GAT'ATC Cuts at: 1294 1294 Size: 6371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2790 4390 2790 Size: 1600 4771 FspI TGC'GCA Cuts at: 4152 4152 Size: 6371 HaeII r_GCGC'y Cuts at: 1494 3805 4053 5283 6054 1494 Size: 2311 248 1230 771 1811 Fragments arranged by size: 2311 1811 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 667 699 1659 1691 4213 4243 4245 4275 667 Size: 32 960 32 2522 30 2 30 2763 Fragments arranged by size: 2763 2522 960 32 32 30 30 2 HincII GTy'rAC Cuts at: 260 2229 3410 260 Size: 1969 1181 3221 Fragments arranged by size: 3221 1969 1181 HindIII A'AGCT_T Cuts at: 1694 1694 Size: 6371 KasI G'GCGC_C Cuts at: 3801 4049 3801 Size: 248 6123 KpnI G_GTAC'C Cuts at: 2369 3391 2369 Size: 1022 5349 MluI A'CGCG_T Cuts at: 5804 5804 Size: 6371 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 212 307 5130 5314 212 Size: 95 4823 184 1269 Fragments arranged by size: 4823 1269 184 95 MscI TGG'CCA Cuts at: 4132 4132 Size: 6371 MslI CAynn'nnrTG Cuts at: 4360 4487 4360 Size: 127 6244 MspA1I CmG'CkG Cuts at: 1144 1327 1707 2790 3482 3796 4156 4940 5185 Size: 183 380 1083 692 314 360 784 245 Cuts at: 5185 1144 Size: 2330 Fragments arranged by size: 2330 1083 784 692 380 360 314 245 183 NaeI GCC'GGC Cuts at: 4552 4552 Size: 6371 NarI GG'CG_CC Cuts at: 3802 4050 3802 Size: 248 6123 NcoI C'CATG_G Cuts at: 107 2732 107 Size: 2625 3746 NdeI CA'TA_TG Cuts at: 1623 1623 Size: 6371 NgoMIV G'CCGG_C Cuts at: 4550 4550 Size: 6371 NheI G'CTAG_C Cuts at: 2299 2299 Size: 6371 NsiI A_TGCA'T Cuts at: 451 1922 451 Size: 1471 4900 NspI r_CATG'y Cuts at: 314 530 1848 2700 3284 3424 3924 5527 314 Size: 216 1318 852 584 140 500 1603 1158 Fragments arranged by size: 1603 1318 1158 852 584 500 216 140 PasI CC'CwG_GG Cuts at: 3264 3264 Size: 6371 PciI A'CATG_T Cuts at: 526 1844 5523 526 Size: 1318 3679 1374 Fragments arranged by size: 3679 1374 1318 PflMI CCAn_nnn'nTGG Cuts at: 426 2733 426 Size: 2307 4064 PfoI T'CCnGG_A Cuts at: 338 1949 338 Size: 1611 4760 PmeI GTTT'AAAC Cuts at: 3370 3370 Size: 6371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2021 2053 4817 4849 2021 Size: 32 2764 32 3543 Fragments arranged by size: 3543 2764 32 32 PpuMI rG'GwC_Cy Cuts at: 416 416 Size: 6371 PshAI GACnn'nnGTC Cuts at: 3353 3353 Size: 6371 PsiI TTA'TAA Cuts at: 2597 2597 Size: 6371 PspOMI G'GGCC_C Cuts at: 1545 1545 Size: 6371 PstI C_TGCA'G Cuts at: 1895 2834 3418 4103 1895 Size: 939 584 685 4163 Fragments arranged by size: 4163 939 685 584 PvuI CG_AT'CG Cuts at: 104 104 Size: 6371 PvuII CAG'CTG Cuts at: 1144 1327 1707 2790 3796 4156 1144 Size: 183 380 1083 1006 360 3359 Fragments arranged by size: 3359 1083 1006 380 360 183 RsrII CG'GwC_CG Cuts at: 4566 4566 Size: 6371 SacI G_AGCT'C Cuts at: 799 3385 799 Size: 2586 3785 SalI G'TCGA_C Cuts at: 3408 3408 Size: 6371 SapI GCTCTTCn'nnn_ Cuts at: 2111 2394 4394 4604 2111 Size: 283 2000 210 3878 Fragments arranged by size: 3878 2000 283 210 SbfI CC_TGCA'GG Cuts at: 3418 3418 Size: 6371 ScaI AGT'ACT Cuts at: 1271 1312 5888 1271 Size: 41 4576 1754 Fragments arranged by size: 4576 1754 41 SfcI C'TryA_G Cuts at: 33 1891 2053 2394 2830 3414 4099 5067 5258 Size: 1858 162 341 436 584 685 968 191 Cuts at: 5258 33 Size: 1146 Fragments arranged by size: 1858 1146 968 685 584 436 341 191 162 SfiI GGCCn_nnn'nGGCC Cuts at: 1749 1749 Size: 6371 SfoI GGC'GCC Cuts at: 3803 4051 3803 Size: 248 6123 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6371 SmaI CCC'GGG Cuts at: 3393 3393 Size: 6371 SmlI C'TyrA_G Cuts at: 153 1086 3526 4441 5155 5417 153 Size: 933 2440 915 714 262 1107 Fragments arranged by size: 2440 1107 933 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6371 SphI G_CATG'C Cuts at: 2700 3424 3924 2700 Size: 724 500 5147 Fragments arranged by size: 5147 724 500 SspI AAT'ATT Cuts at: 1909 3054 1909 Size: 1145 5226 StyI C'CwwG_G Cuts at: 107 2732 2774 3505 3859 107 Size: 2625 42 731 354 2619 Fragments arranged by size: 2625 2619 731 354 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2272 2512 2623 2930 3954 4087 4546 4755 2272 Size: 240 111 307 1024 133 459 209 3888 Fragments arranged by size: 3888 1024 459 307 240 209 133 111 TatI w'GTAC_w Cuts at: 1239 1269 1310 3068 3111 5886 1239 Size: 30 41 1758 43 2775 1724 Fragments arranged by size: 2775 1758 1724 43 41 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 605 637 1787 1819 5888 5920 605 Size: 32 1150 32 4069 32 1056 Fragments arranged by size: 4069 1150 1056 32 32 32 Tth111I GACn'n_nGTC Cuts at: 4168 4168 Size: 6371 XbaI T'CTAG_A Cuts at: 822 3156 3402 822 Size: 2334 246 3791 Fragments arranged by size: 3791 2334 246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3866 3866 Size: 6371 XmaI C'CCGG_G Cuts at: 3391 3391 Size: 6371 XmnI GAAnn'nnTTC Cuts at: 1661 2120 2267 1661 Size: 459 147 5765 Fragments arranged by size: 5765 459 147 ZraI GAC'GTC Cuts at: 1927 1927 Size: 6371 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AhdI AleI AlfI AscI AvrII BaeI BcgI BmgBI BsiWI BspEI BsrGI BssHII CspCI EcoNI FseI FspAI HpaI MfeI NotI NruI PacI PmlI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI StuI SwaI XhoI