(Linear) (Six-base) MAPSORT of: KIBB9559.seq Check: 2047 from: 1 to: 3258 KIBB9559.orf With 182 enzymes: SgfI * March 3, 2008 11:10 .. AatII G_ACGT'C Cuts at: 0 1821 3258 Size: 1821 1437 AccI GT'mk_AC Cuts at: 0 1944 2214 3258 Size: 1944 270 1044 Fragments arranged by size: 1944 1044 270 Acc65I G'GTAC_C Cuts at: 0 2257 3258 Size: 2257 1001 AclI AA'CG_TT Cuts at: 0 972 3258 Size: 972 2286 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 2623 3258 Size: 472 2151 635 Fragments arranged by size: 2151 635 472 AfeI AGC'GCT Cuts at: 0 1384 3258 Size: 1384 1874 AflII C'TTAA_G Cuts at: 0 45 3258 Size: 45 3213 AflIII A'CryG_T Cuts at: 0 418 1736 2933 3258 Size: 418 1318 1197 325 Fragments arranged by size: 1318 1197 418 325 AgeI A'CCGG_T Cuts at: 0 181 3258 Size: 181 3077 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1913 1945 2525 2557 2627 2659 3258 Size: 1913 32 580 32 70 32 599 Fragments arranged by size: 1913 599 580 70 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 859 2709 2823 3258 Size: 859 1850 114 435 Fragments arranged by size: 1850 859 435 114 ApaI G_GGCC'C Cuts at: 0 1441 3258 Size: 1441 1817 ApoI r'AATT_y Cuts at: 0 350 1069 2020 2156 2910 3028 3079 3258 Size: 350 719 951 136 754 118 51 179 Fragments arranged by size: 951 754 719 350 179 136 118 51 AseI AT'TA_AT Cuts at: 0 83 725 3258 Size: 83 642 2533 Fragments arranged by size: 2533 642 83 AvaI C'yCGr_G Cuts at: 0 1657 3019 3258 Size: 1657 1362 239 Fragments arranged by size: 1657 1362 239 BamHI G'GATC_C Cuts at: 0 402 3258 Size: 402 2856 BanI G'GyrC_C Cuts at: 0 2257 2416 3258 Size: 2257 159 842 Fragments arranged by size: 2257 842 159 BanII G_rGCy'C Cuts at: 0 691 1441 3258 Size: 691 750 1817 Fragments arranged by size: 1817 750 691 BbsI GAAGACnn'nnnn_ Cuts at: 0 26 415 1853 2936 3258 Size: 26 389 1438 1083 322 Fragments arranged by size: 1438 1083 389 322 26 BbvCI CC'TCA_GC Cuts at: 0 1568 2870 3258 Size: 1568 1302 388 Fragments arranged by size: 1568 1302 388 BciVI GTATCCnnnnn_n' Cuts at: 0 3006 3258 Size: 3006 252 BclI T'GATC_A Cuts at: 0 2268 3258 Size: 2268 990 BfrBI ATG'CAT Cuts at: 0 341 1812 3258 Size: 341 1471 1446 Fragments arranged by size: 1471 1446 341 BglI GCCn_nnn'nGGC Cuts at: 0 1641 3258 Size: 1641 1617 BglII A'GATC_T Cuts at: 0 3045 3258 Size: 3045 213 Bme1580I G_kGCm'C Cuts at: 0 1441 3258 Size: 1441 1817 BmrI ACTGGGnnnn_n' Cuts at: 0 2110 3258 Size: 2110 1148 BmtI G_CTAG'C Cuts at: 0 2195 3258 Size: 2195 1063 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 370 3258 Size: 370 2888 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 532 1142 1982 2845 3258 Size: 340 192 610 840 863 413 Fragments arranged by size: 863 840 610 413 340 192 Bpu10I CC'TnA_GC Cuts at: 0 1124 1568 2870 3258 Size: 1124 444 1302 388 Fragments arranged by size: 1302 1124 444 388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 999 3258 Size: 999 2259 BsaI GGTCTCn'nnnn_ Cuts at: 0 190 579 3258 Size: 190 389 2679 Fragments arranged by size: 2679 389 190 BsaHI Gr'CG_yC Cuts at: 0 1818 2760 3258 Size: 1818 942 498 Fragments arranged by size: 1818 942 498 BsaWI w'CCGG_w Cuts at: 0 181 3258 Size: 181 3077 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 497 527 1518 1548 1913 1943 1955 1985 Size: 497 30 991 30 365 30 12 30 Cuts at: 1985 3258 Size: 1273 Fragments arranged by size: 1273 991 497 365 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1526 1934 3140 3258 Size: 1526 408 1206 118 Fragments arranged by size: 1526 1206 408 118 BseYI C'CCAG_C Cuts at: 0 253 1599 2208 2854 3258 Size: 253 1346 609 646 404 Fragments arranged by size: 1346 646 609 404 253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2351 2413 3258 Size: 2351 62 845 Fragments arranged by size: 2351 845 62 BsiHKAI G_wGCw'C Cuts at: 0 326 691 820 2094 3258 Size: 326 365 129 1274 1164 Fragments arranged by size: 1274 1164 365 326 129 BsmI GAATG_Cn' Cuts at: 0 1679 1782 3258 Size: 1679 103 1476 Fragments arranged by size: 1679 1476 103 BsmBI CGTCTCn'nnnn_ Cuts at: 0 30 3258 Size: 30 3228 Bsp1286I G_dGCh'C Cuts at: 0 326 691 820 1441 2094 3258 Size: 326 365 129 621 653 1164 Fragments arranged by size: 1164 653 621 365 326 129 BsrDI GCAATG_nn' Cuts at: 0 154 3258 Size: 154 3104 BsrFI r'CCGG_y Cuts at: 0 181 1865 3258 Size: 181 1684 1393 Fragments arranged by size: 1684 1393 181 BstEII G'GTnAC_C Cuts at: 0 211 3258 Size: 211 3047 BstXI CCAn_nnnn'nTGG Cuts at: 0 2208 3006 3258 Size: 2208 798 252 Fragments arranged by size: 2208 798 252 BstYI r'GATC_y Cuts at: 0 227 402 780 1008 1146 2642 3045 3258 Size: 227 175 378 228 138 1496 403 213 Fragments arranged by size: 1496 403 378 228 227 213 175 138 BstZ17I GTA'TAC Cuts at: 0 2215 3258 Size: 2215 1043 Bsu36I CC'TnA_GG Cuts at: 0 540 3258 Size: 540 2718 BtgI C'CryG_G Cuts at: 0 2624 2646 3258 Size: 2624 22 612 Fragments arranged by size: 2624 612 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1311 3258 Size: 1311 1947 BtsI GCAGTG_nn' Cuts at: 0 506 3258 Size: 506 2752 ClaI AT'CG_AT Cuts at: 0 2768 3258 Size: 2768 490 DraI TTT'AAA Cuts at: 0 969 3258 Size: 969 2289 DraIII CAC_nnn'GTG Cuts at: 0 2692 3258 Size: 2692 566 DrdI GACnn_nn'nnGTC Cuts at: 0 634 3058 3258 Size: 634 2424 200 Fragments arranged by size: 2424 634 200 EaeI y'GGCC_r Cuts at: 0 1281 3258 Size: 1281 1977 EarI CTCTTCn'nnn_ Cuts at: 0 2003 2286 3258 Size: 2003 283 972 Fragments arranged by size: 2003 972 283 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2666 3258 Size: 2666 592 EcoICRI GAG'CTC Cuts at: 0 689 3258 Size: 689 2569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 472 532 1142 1982 2623 2845 3258 Size: 340 132 60 610 840 641 222 413 Fragments arranged by size: 840 641 610 413 340 222 132 60 EcoO109I rG'GnC_Cy Cuts at: 0 257 308 1437 2233 3258 Size: 257 51 1129 796 1025 Fragments arranged by size: 1129 1025 796 257 51 EcoRI G'AATT_C Cuts at: 0 350 3258 Size: 350 2908 EcoRV GAT'ATC Cuts at: 0 1186 3258 Size: 1186 2072 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2682 3258 Size: 2682 576 HaeII r_GCGC'y Cuts at: 0 1386 3258 Size: 1386 1872 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 559 591 1551 1583 3258 Size: 559 32 960 32 1675 Fragments arranged by size: 1675 960 559 32 32 HincII GTy'rAC Cuts at: 0 152 2121 3258 Size: 152 1969 1137 Fragments arranged by size: 1969 1137 152 HindIII A'AGCT_T Cuts at: 0 1586 3258 Size: 1586 1672 KpnI G_GTAC'C Cuts at: 0 2261 3258 Size: 2261 997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 104 199 3258 Size: 104 95 3059 Fragments arranged by size: 3059 104 95 MspA1I CmG'CkG Cuts at: 0 1036 1219 1599 2682 3258 Size: 1036 183 380 1083 576 Fragments arranged by size: 1083 1036 576 380 183 NcoI C'CATG_G Cuts at: 0 2624 3258 Size: 2624 634 NdeI CA'TA_TG Cuts at: 0 1515 3258 Size: 1515 1743 NheI G'CTAG_C Cuts at: 0 2191 3258 Size: 2191 1067 NsiI A_TGCA'T Cuts at: 0 343 1814 3258 Size: 343 1471 1444 Fragments arranged by size: 1471 1444 343 NspI r_CATG'y Cuts at: 0 206 422 1740 2592 3176 3258 Size: 206 216 1318 852 584 82 Fragments arranged by size: 1318 852 584 216 206 82 PasI CC'CwG_GG Cuts at: 0 3156 3258 Size: 3156 102 PciI A'CATG_T Cuts at: 0 418 1736 3258 Size: 418 1318 1522 Fragments arranged by size: 1522 1318 418 PflMI CCAn_nnn'nTGG Cuts at: 0 318 2625 3258 Size: 318 2307 633 Fragments arranged by size: 2307 633 318 PfoI T'CCnGG_A Cuts at: 0 230 1841 3258 Size: 230 1611 1417 Fragments arranged by size: 1611 1417 230 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1913 1945 3258 Size: 1913 32 1313 Fragments arranged by size: 1913 1313 32 PpuMI rG'GwC_Cy Cuts at: 0 308 3258 Size: 308 2950 PshAI GACnn'nnGTC Cuts at: 0 3245 3258 Size: 3245 13 PsiI TTA'TAA Cuts at: 0 2489 3258 Size: 2489 769 PspOMI G'GGCC_C Cuts at: 0 1437 3258 Size: 1437 1821 PstI C_TGCA'G Cuts at: 0 1787 2726 3258 Size: 1787 939 532 Fragments arranged by size: 1787 939 532 PvuII CAG'CTG Cuts at: 0 1036 1219 1599 2682 3258 Size: 1036 183 380 1083 576 Fragments arranged by size: 1083 1036 576 380 183 SacI G_AGCT'C Cuts at: 0 691 3258 Size: 691 2567 SapI GCTCTTCn'nnn_ Cuts at: 0 2003 2286 3258 Size: 2003 283 972 Fragments arranged by size: 2003 972 283 ScaI AGT'ACT Cuts at: 0 1163 1204 3258 Size: 1163 41 2054 Fragments arranged by size: 2054 1163 41 SfcI C'TryA_G Cuts at: 0 1783 1945 2286 2722 3258 Size: 1783 162 341 436 536 Fragments arranged by size: 1783 536 436 341 162 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1641 3258 Size: 1641 1617 SmlI C'TyrA_G Cuts at: 0 45 978 3258 Size: 45 933 2280 Fragments arranged by size: 2280 933 45 SphI G_CATG'C Cuts at: 0 2592 3258 Size: 2592 666 SspI AAT'ATT Cuts at: 0 1801 2946 3258 Size: 1801 1145 312 Fragments arranged by size: 1801 1145 312 StyI C'CwwG_G Cuts at: 0 2624 2666 3258 Size: 2624 42 592 Fragments arranged by size: 2624 592 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2164 2404 2515 2822 3258 Size: 2164 240 111 307 436 Fragments arranged by size: 2164 436 307 240 111 TatI w'GTAC_w Cuts at: 0 1131 1161 1202 2960 3003 3258 Size: 1131 30 41 1758 43 255 Fragments arranged by size: 1758 1131 255 43 41 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 497 529 1679 1711 3258 Size: 497 32 1150 32 1547 Fragments arranged by size: 1547 1150 497 32 32 XbaI T'CTAG_A Cuts at: 0 714 3048 3258 Size: 714 2334 210 Fragments arranged by size: 2334 714 210 XmnI GAAnn'nnTTC Cuts at: 0 1553 2012 2159 3258 Size: 1553 459 147 1099 Fragments arranged by size: 1553 1099 459 147 ZraI GAC'GTC Cuts at: 0 1819 3258 Size: 1819 1439 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AloI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KpnI MmeI MspA1I NcoI NdeI NheI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SapI ScaI SfcI SfiI SmlI SphI SspI StyI TaqII TatI TstI XbaI XmnI ZraI Enzymes that do not cut: AarI AhdI AleI AlfI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BlpI BmgBI BsaAI BsaBI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI CspCI EagI EcoNI FseI FspI FspAI HpaI KasI MfeI MluI MscI MslI NaeI NarI NgoMIV NotI NruI PacI PmeI PmlI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XcmI XhoI XmaI