Restriction map for pF1KB9557
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

Acc65I      AccIII      AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
BbrPI       BcgI        BfrI        BglII       BsaAI       
BsaWI       BseAI       BsiEI       BsiWI       BspEI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoT22I     FseI        
FspI        HpaI        KpnI        KspI        MluI        
MroI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       PvuI        RsrII       
SacI        SacII       SbfI        ScaI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          929  GTCGAC               GT^[AC][GT]AC
AccII        5665  CGCG                 CG^CG
AspHI        4671  GAGCAC               G[AT]GC[AT]^C
AspI         3497  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         934  CCTAGG               C^CTAGG
BamHI        4549  GGATCC               G^GATCC
BbeI         4321  GGCGCC               GGCGC^C
BciVI        5844  GTATCC               GTATCC(6/5)
BclI         5147  TGATCA               T^GATCA
BcnI         2244  CCGGG                CC^[GC]GG
BglI         5647  GCCATGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          934  CCTAGG               C^CTAGG
BlpI         5374  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     5374  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2302  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      4671  GAGCAC               G[AT]GC[AT]^C
BsmBI        2668  GAGACG               CGTCTC(1/5)
BspLU11I     5492  ACATGT               A^CATGT
BssSI        4008  CACGAG               CACGAG(-5/-1)
BstBI        1067  TTCGAA               TT^CGAA
BstUI        5665  CGCG                 CG^CG
CelII        5374  GCTCAGC              GC^T[ACGT]AGC
DrdI         2929  GACAGGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        3654  CCTACCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        3402  GAATTC               G^AATTC
EcoRV        1459  GATATC               GAT^ATC
FbaI         5147  TGATCA               T^GATCA
HindIII      2275  AAGCTT               A^AGCTT
KasI         4321  GGCGCC               G^GCGCC
MamI         2302  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          148  CAATTG               C^AATTG
MunI          148  CAATTG               C^AATTG
MvnI         5665  CGCG                 CG^CG
NaeI         5221  GCCGGC               GCC^GGC
NarI         4321  GGCGCC               GG^CGCC
NciI         2244  CCGGG                CC^[GC]GG
NgoMIV       5221  GCCGGC               G^CCGGC
NspV         1067  TTCGAA               TT^CGAA
PflFI        3497  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2328  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI          929  GTCGAC               G^TCGAC
SfoI         4321  GGCGCC               GGC^GCC
SfuI         1067  TTCGAA               TT^CGAA
SphI         3556  GCATGC               GCATG^C
Tth111I      3497  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AatII        1007  GACGTC               GACGT^C
             2670  GACGTC              
AclI          365  AACGTT               AA^CGTT
             2630  AACGTT              
Asp700I      1447  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4237  GAAGAGATTC          
AvaI         1277  CTCGGG               C^[CT]CG[GA]G
             3658  CCCGAG              
BanI         4321  GGCGCC               G^G[CT][GA]CC
             5218  GGTGCC              
BbvCI        5053  CCTCAGC              CCTCAGC(-5/-2)
             2380  GCTGAGG             
BsgI         2877  GTGCAG               GTGCAG(16/14)
             4282  GTGCAG              
BsoBI        1277  CTCGGG               C^[CT]CG[GA]G
             3658  CCCGAG              
Bsp1407I     4272  TGTACA               T^GTACA
             5930  TGTACA              
BspDI        1987  ATCGAT               AT^CGAT
             3199  ATCGAT              
BspMI        1976  GCAGGT               ACCTGC(4/8)
             4629  GCAGGT              
BsrBI        5571  CCGCTC               CCGCTC(-3/-3)
             1153  GAGCGG              
BsrDI          98  GCAATG               GCAATG(2/0)
             1187  GCAATG              
BsrFI         600  GCCGGT               [GA]^CCGG[CT]
             5221  GCCGGC              
BsrGI        4272  TGTACA               T^GTACA
             5930  TGTACA              
Cfr10I        600  GCCGGT               [GA]^CCGG[CT]
             5221  GCCGGC              
ClaI         1987  ATCGAT               AT^CGAT
             3199  ATCGAT              
DraI          180  TTTAAA               TTT^AAA
              723  TTTAAA              
HaeII         986  GGCGCT               [GA]GCGC^[CT]
             4321  GGCGCC              
HincII        929  GTCGAC               GT[CT]^[GA]AC
             2154  GTTGAC              
HindII        929  GTCGAC               GT[CT]^[GA]AC
             2154  GTTGAC              
PflMI         117  CCATGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5161  CCAGAATCTGG         
PpuMI        1050  AGGTCCC              [GA]G^G[AT]CC[CT]
             4391  AGGACCC             
Psp1406I      365  AACGTT               AA^CGTT
             2630  AACGTT              
SapI         2398  GAAGAGC              GCTCTTC(1/4)
             4742  GCTCTTC             
SexAI        1561  ACCTGGT              A^CC[AT]GGT
             3498  ACCTGGT             
SspBI        4272  TGTACA               T^GTACA
             5930  TGTACA              
SspI         1723  AATATT               AAT^ATT
             2094  AATATT              
Van91I        117  CCATGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5161  CCAGAATCTGG         
XmnI         1447  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4237  GAAGAGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
AvaII       BalI        BanII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspHI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       
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