Restriction map for pF1KB9557
 (Linear) (Six-base) MAPSORT of: KIBB9557.seq  Check: 6569  from: 1  to: 6012



KIBB9557.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4620    6012 
   Size:       4620    1392
 
AatII G_ACGT'C

Cuts at:      0    1011    2674    6012 
   Size:       1011    1663    3338

  Fragments arranged by size: 

                3338    1663    1011
 
AccI GT'mk_AC

Cuts at:      0     930    6012 
   Size:        930    5082
 
AclI AA'CG_TT

Cuts at:      0     366    2631    6012 
   Size:        366    2265    3381

  Fragments arranged by size: 

                3381    2265     366
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65    1041    1331    2263    2660    2893    2934    3049 
   Size:         65     976     290     932     397     233      41     115

Cuts at:   3049    3146    3728    4632    5235    5467    6012 
   Size:         97     582     904     603     232     545

  Fragments arranged by size: 

                 976     932     904     603     582     545     397     290
                 233     232     115      97      65      41
 
AflIII A'CryG_T

Cuts at:      0    2530    4142    5492    6012 
   Size:       2530    1612    1350     520

  Fragments arranged by size: 

                2530    1612    1350     520
 
AleI CACnn'nnGTG

Cuts at:      0    3127    4147    6012 
   Size:       3127    1020    1865

  Fragments arranged by size: 

                3127    1865    1020
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4870    6012 
   Size:       4870    1142
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4729    4761    6012 
   Size:       4729      32    1251

  Fragments arranged by size: 

                4729    1251      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     337     800    3922    4365    4730    4844    5167    5274 
   Size:        337     463    3122     443     365     114     323     107

Cuts at:   5274    5619    6012 
   Size:        345     393

  Fragments arranged by size: 

                3122     463     443     393     365     345     337     323
                 114     107
 
ApoI r'AATT_y

Cuts at:      0    1243    1553    1621    2286    3402    6012 
   Size:       1243     310      68     665    1116    2610

  Fragments arranged by size: 

                2610    1243    1116     665     310      68
 
AvaI C'yCGr_G

Cuts at:      0    1277    3658    6012 
   Size:       1277    2381    2354

  Fragments arranged by size: 

                2381    2354    1277
 
AvrII C'CTAG_G

Cuts at:      0     934    6012 
   Size:        934    5078
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    5172    5205    6012 
   Size:       5172      33     807

  Fragments arranged by size: 

                5172     807      33
 
BamHI G'GATC_C

Cuts at:      0    4549    6012 
   Size:       4549    1463
 
BanI G'GyrC_C

Cuts at:      0    4321    5218    6012 
   Size:       4321     897     794

  Fragments arranged by size: 

                4321     897     794
 
BanII G_rGCy'C

Cuts at:      0    1226    2837    3572    6012 
   Size:       1226    1611     735    2440

  Fragments arranged by size: 

                2440    1611    1226     735
 
BbeI G_GCGC'C

Cuts at:      0    4325    6012 
   Size:       4325    1687
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     101    2557    2666    2820    3257    3650    4199    4421 
   Size:        101    2456     109     154     437     393     549     222

Cuts at:   4421    6012 
   Size:       1591

  Fragments arranged by size: 

                2456    1591     549     437     393     222     154     109
                 101
 
BbvCI CC'TCA_GC

Cuts at:      0    2381    5054    6012 
   Size:       2381    2673     958

  Fragments arranged by size: 

                2673    2381     958
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    5855    6012 
   Size:       5855     157
 
BclI T'GATC_A

Cuts at:      0    5147    6012 
   Size:       5147     865
 
BglI GCCn_nnn'nGGC

Cuts at:      0    5653    6012 
   Size:       5653     359
 
BlpI GC'TnA_GC

Cuts at:      0    5375    6012 
   Size:       5375     637
 
Bme1580I G_kGCm'C

Cuts at:      0    5561    5978    6012 
   Size:       5561     417      34

  Fragments arranged by size: 

                5561     417      34
 
BmgBI CAC'GTC

Cuts at:      0    4145    6012 
   Size:       4145    1867
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4925    5186    6012 
   Size:       4925     261     826

  Fragments arranged by size: 

                4925     826     261
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2866    6012 
   Size:       2866    3146
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     551    2018    2315    2501    3743    3991    4767    6012 
   Size:        551    1467     297     186    1242     248     776    1245

  Fragments arranged by size: 

                1467    1245    1242     776     551     297     248     186
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     248    2381    5054    5397    6012 
   Size:        248    2133    2673     343     615

  Fragments arranged by size: 

                2673    2133     615     343     248
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1568    1578    2156    2836    3433    3536    4280    4534 
   Size:       1568      10     578     680     597     103     744     254

Cuts at:   4534    5396    6012 
   Size:        862     616

  Fragments arranged by size: 

                1568     862     744     680     616     597     578     254
                 103      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2036    2361    3112    3778    4576    6012 
   Size:       2036     325     751     666     798    1436

  Fragments arranged by size: 

                2036    1436     798     751     666     325
 
BsaBI GATnn'nnATC

Cuts at:      0    2306    6012 
   Size:       2306    3706
 
BsaHI Gr'CG_yC

Cuts at:      0    1008    2671    4322    5431    6012 
   Size:       1008    1663    1651    1109     581

  Fragments arranged by size: 

                1663    1651    1109    1008     581
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3032    3062    5124    5154    6012 
   Size:       3032      30    2062      30     858

  Fragments arranged by size: 

                3032    2062     858      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1414    1560    2377    2389    2672    2785    2980    2983 
   Size:       1414     146     817      12     283     113     195       3

Cuts at:   2983    2986    2989    3075    3226    3398    3676    3688    3691 
   Size:          3       3      86     151     172     278      12       3

Cuts at:   3691    3694    3826    3856    3874    3877    3895    3910    3913 
   Size:          3     132      30      18       3      18      15       3

Cuts at:   3913    4042    4138    4246    4440    5399    5465    6012 
   Size:        129      96     108     194     959      66     547

  Fragments arranged by size: 

                1414     959     817     547     283     278     195     194
                 172     151     146     132     129     113     108      96
                  86      66      30      18      18      15      12      12
                   3       3       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1376    2600    3747    4168    4468    5024    5060    5087 
   Size:       1376    1224    1147     421     300     556      36      27

Cuts at:   5087    5136    5401    5891    6012 
   Size:         49     265     490     121

  Fragments arranged by size: 

                1376    1224    1147     556     490     421     300     265
                 121      49      36      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2898    4303    6012 
   Size:       2898    1405    1709

  Fragments arranged by size: 

                2898    1709    1405
 
BsiHKAI G_wGCw'C

Cuts at:      0    4675    6012 
   Size:       4675    1337
 
BsmI GAATG_Cn'

Cuts at:      0    2210    5513    5763    6012 
   Size:       2210    3303     250     249

  Fragments arranged by size: 

                3303    2210     250     249
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2662    6012 
   Size:       2662    3350
 
Bsp1286I G_dGCh'C

Cuts at:      0    1226    2837    3572    4675    5561    5978    6012 
   Size:       1226    1611     735    1103     886     417      34

  Fragments arranged by size: 

                1611    1226    1103     886     735     417      34
 
BspHI T'CATG_A

Cuts at:      0    1427    2454    2556    6012 
   Size:       1427    1027     102    3456

  Fragments arranged by size: 

                3456    1427    1027     102
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1967    4620    6012 
   Size:       1967    2653    1392

  Fragments arranged by size: 

                2653    1967    1392
 
BsrBI CCG'CTC

Cuts at:      0    1155    5573    6012 
   Size:       1155    4418     439

  Fragments arranged by size: 

                4418    1155     439
 
BsrDI GCAATG_nn'

Cuts at:      0     105    1194    6012 
   Size:        105    1089    4818

  Fragments arranged by size: 

                4818    1089     105
 
BsrFI r'CCGG_y

Cuts at:      0     600    5221    6012 
   Size:        600    4621     791

  Fragments arranged by size: 

                4621     791     600
 
BsrGI T'GTAC_A

Cuts at:      0    4272    5930    6012 
   Size:       4272    1658      82

  Fragments arranged by size: 

                4272    1658      82
 
BssSI C'ACGA_G

Cuts at:      0    4008    6012 
   Size:       4008    2004
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3922    4844    5532    5901    6012 
   Size:       3922     922     688     369     111

  Fragments arranged by size: 

                3922     922     688     369     111
 
BstBI TT'CG_AA

Cuts at:      0    1068    6012 
   Size:       1068    4944
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1885    2352    3485    6012 
   Size:       1885     467    1133    2527

  Fragments arranged by size: 

                2527    1885    1133     467
 
BstYI r'GATC_y

Cuts at:      0     216    2307    3608    4260    4549    6012 
   Size:        216    2091    1301     652     289    1463

  Fragments arranged by size: 

                2091    1463    1301     652     289     216
 
Bsu36I CC'TnA_GG

Cuts at:      0    2684    2864    2941    3803    6012 
   Size:       2684     180      77     862    2209

  Fragments arranged by size: 

                2684    2209     862     180      77
 
BtgI C'CryG_G

Cuts at:      0     117     261     468    4148    4406    6012 
   Size:        117     144     207    3680     258    1606

  Fragments arranged by size: 

                3680    1606     258     207     144     117
 
BtsI GCAGTG_nn'

Cuts at:      0     417     778    1474    1908    3099    3744    4286    4314 
   Size:        417     361     696     434    1191     645     542      28

Cuts at:   4314    5587    6012 
   Size:       1273     425

  Fragments arranged by size: 

                1273    1191     696     645     542     434     425     417
                 361      28
 
ClaI AT'CG_AT

Cuts at:      0    1988    3200    6012 
   Size:       1988    1212    2812

  Fragments arranged by size: 

                2812    1988    1212
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     506     541    5490    5525    6012 
   Size:        506      35    4949      35     487

  Fragments arranged by size: 

                4949     506     487      35      35
 
DraI TTT'AAA

Cuts at:      0     182     725    6012 
   Size:        182     543    5287

  Fragments arranged by size: 

                5287     543     182
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2935    6012 
   Size:       2935    3077
 
EaeI y'GGCC_r

Cuts at:      0     465     708    3963    4826    5625    6012 
   Size:        465     243    3255     863     799     387

  Fragments arranged by size: 

                3255     863     799     465     387     243
 
EarI CTCTTCn'nnn_

Cuts at:      0    2366    2393    2660    3203    3212    3300    3326    3695 
   Size:       2366      27     267     543       9      88      26     369

Cuts at:   3695    3704    3716    3815    4066    4115    4205    4232    4749 
   Size:          9      12      99     251      49      90      27     517

Cuts at:   4749    6012 
   Size:       1263

  Fragments arranged by size: 

                2366    1263     543     517     369     267     251      99
                  90      88      49      27      27      26      12       9
                   9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     872    1284    2230    6012 
   Size:        872     412     946    3782

  Fragments arranged by size: 

                3782     946     872     412
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     551    1041    1331    2018    2263    2315    2501 
   Size:         65     486     490     290     687     245      52     186

Cuts at:   2501    2660    2893    2934    3049    3146    3728    3743    3991 
   Size:        159     233      41     115      97     582      15     248

Cuts at:   3991    4632    4767    5235    5467    6012 
   Size:        641     135     468     232     545

  Fragments arranged by size: 

                 687     641     582     545     490     486     468     290
                 248     245     233     232     186     159     135     115
                  97      65      52      41      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3658    6012 
   Size:       3658    2354
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1051    4392    5938    6012 
   Size:       1051    3341    1546      74

  Fragments arranged by size: 

                3341    1546    1051      74
 
EcoRI G'AATT_C

Cuts at:      0    3402    6012 
   Size:       3402    2610
 
EcoRV GAT'ATC

Cuts at:      0    1461    6012 
   Size:       1461    4551
 
HaeII r_GCGC'y

Cuts at:      0     990    4325    6012 
   Size:        990    3335    1687

  Fragments arranged by size: 

                3335    1687     990
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     434     466    1935    1967    3142    3174    3319    3351 
   Size:        434      32    1469      32    1175      32     145      32

Cuts at:   3351    5700    5732    6012 
   Size:       2349      32     280

  Fragments arranged by size: 

                2349    1469    1175     434     280     145      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     931    2156    6012 
   Size:        931    1225    3856

  Fragments arranged by size: 

                3856    1225     931
 
HindIII A'AGCT_T

Cuts at:      0    2275    6012 
   Size:       2275    3737
 
KasI G'GCGC_C

Cuts at:      0    4321    6012 
   Size:       4321    1691
 
MfeI C'AATT_G

Cuts at:      0     148    6012 
   Size:        148    5864
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1467    2369    6012 
   Size:       1467     902    3643

  Fragments arranged by size: 

                3643    1467     902
 
MscI TGG'CCA

Cuts at:      0     467     710    3965    4828    6012 
   Size:        467     243    3255     863    1184

  Fragments arranged by size: 

                3255    1184     863     467     243
 
MslI CAynn'nnrTG

Cuts at:      0     895    2117    3127    4147    5287    5458    5692    5743 
   Size:        895    1222    1010    1020    1140     171     234      51

Cuts at:   5743    6012 
   Size:        269

  Fragments arranged by size: 

                1222    1140    1020    1010     895     269     234     171
                  51
 
MspA1I CmG'CkG

Cuts at:      0    1520    2283    2600    2757    3913    4643    4757    4805 
   Size:       1520     763     317     157    1156     730     114      48

Cuts at:   4805    4844    5103    5274    5379    5619    6012 
   Size:         39     259     171     105     240     393

  Fragments arranged by size: 

                1520    1156     763     730     393     317     259     240
                 171     157     114     105      48      39
 
NaeI GCC'GGC

Cuts at:      0    5223    6012 
   Size:       5223     789
 
NarI GG'CG_CC

Cuts at:      0    4322    6012 
   Size:       4322    1690
 
NcoI C'CATG_G

Cuts at:      0     117     261    4406    6012 
   Size:        117     144    4145    1606

  Fragments arranged by size: 

                4145    1606     144     117
 
NgoMIV G'CCGG_C

Cuts at:      0    5221    6012 
   Size:       5221     791
 
NspI r_CATG'y

Cuts at:      0     794     834     912    3560    3651    5496    6012 
   Size:        794      40      78    2648      91    1845     516

  Fragments arranged by size: 

                2648    1845     794     516      91      78      40
 
PciI A'CATG_T

Cuts at:      0    5492    6012 
   Size:       5492     520
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     123    5167    6012 
   Size:        123    5044     845

  Fragments arranged by size: 

                5044     845     123
 
PfoI T'CCnGG_A

Cuts at:      0    2258    3611    6012 
   Size:       2258    1353    2401

  Fragments arranged by size: 

                2401    2258    1353
 
PpuMI rG'GwC_Cy

Cuts at:      0    1051    4392    6012 
   Size:       1051    3341    1620

  Fragments arranged by size: 

                3341    1620    1051
 
PshAI GACnn'nnGTC

Cuts at:      0    2332    6012 
   Size:       2332    3680
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    5788    5820    5914    5946    6012 
   Size:       5788      32      94      32      66

  Fragments arranged by size: 

                5788      94      66      32      32
 
PstI C_TGCA'G

Cuts at:      0    1180    1481    1661    3918    4370    4849    4891    5270 
   Size:       1180     301     180    2257     452     479      42     379

Cuts at:   5270    6012 
   Size:        742

  Fragments arranged by size: 

                2257    1180     742     479     452     379     301     180
                  42
 
PvuII CAG'CTG

Cuts at:      0    2283    2757    3913    4757    4805    4844    5274    5379 
   Size:       2283     474    1156     844      48      39     430     105

Cuts at:   5379    5619    6012 
   Size:        240     393

  Fragments arranged by size: 

                2283    1156     844     474     430     393     240     105
                  48      39
 
SalI G'TCGA_C

Cuts at:      0     929    6012 
   Size:        929    5083
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2393    4749    6012 
   Size:       2393    2356    1263

  Fragments arranged by size: 

                2393    2356    1263
 
SexAI A'CCwGG_T

Cuts at:      0    1561    3498    6012 
   Size:       1561    1937    2514

  Fragments arranged by size: 

                2514    1937    1561
 
SfcI C'TryA_G

Cuts at:      0     624    1176    1477    1657    2297    2916    3914    4366 
   Size:        624     552     301     180     640     619     998     452

Cuts at:   4366    4845    4887    5266    6012 
   Size:        479      42     379     746

  Fragments arranged by size: 

                 998     746     640     624     619     552     479     452
                 379     301     180      42
 
SfoI GGC'GCC

Cuts at:      0    4323    6012 
   Size:       4323    1689
 
SmlI C'TyrA_G

Cuts at:      0    1583    1593    2171    2851    3412    3551    4295    4513 
   Size:       1583      10     578     680     561     139     744     218

Cuts at:   4513    5411    6012 
   Size:        898     601

  Fragments arranged by size: 

                1583     898     744     680     601     578     561     218
                 139      10
 
SphI G_CATG'C

Cuts at:      0    3560    6012 
   Size:       3560    2452
 
SspI AAT'ATT

Cuts at:      0    1725    2096    6012 
   Size:       1725     371    3916

  Fragments arranged by size: 

                3916    1725     371
 
StuI AGG'CCT

Cuts at:      0     760    4926    5884    6012 
   Size:        760    4166     958     128

  Fragments arranged by size: 

                4166     958     760     128
 
StyI C'CwwG_G

Cuts at:      0     117     261     934    3794    4406    6012 
   Size:        117     144     673    2860     612    1606

  Fragments arranged by size: 

                2860    1606     673     612     144     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     869    2740    2981    3802    4393    4476    4543    4810 
   Size:        869    1871     241     821     591      83      67     267

Cuts at:   4810    6012 
   Size:       1202

  Fragments arranged by size: 

                1871    1202     869     821     591     267     241      83
                  67
 
TatI w'GTAC_w

Cuts at:      0    1848    1922    3276    4272    5930    6012 
   Size:       1848      74    1354     996    1658      82

  Fragments arranged by size: 

                1848    1658    1354     996      82      74
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2432    2464    3958    3990    5124    5156    6012 
   Size:       2432      32    1494      32    1134      32     856

  Fragments arranged by size: 

                2432    1494    1134     856      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3500    6012 
   Size:       3500    2512
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     908    2632    4593    5286    5367    6012 
   Size:        908    1724    1961     693      81     645

  Fragments arranged by size: 

                1961    1724     908     693     645      81
 
XmnI GAAnn'nnTTC

Cuts at:      0    1451    4241    6012 
   Size:       1451    2790    1771

  Fragments arranged by size: 

                2790    1771    1451
 
ZraI GAC'GTC

Cuts at:      0    1009    2672    6012 
   Size:       1009    1663    3340

  Fragments arranged by size: 

                3340    1663    1009
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI   AflIII     AleI     AlfI
     AloI    AlwNI     ApoI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII  HindIII
     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI    PpuMI    PshAI
     PsrI     PstI    PvuII     SalI     SapI    SexAI     SfcI     SfoI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI    AflII     AgeI     AhdI     ApaI    ApaLI     AscI
     AseI    AsiSI     BcgI    BfrBI    BglII     BmtI    BsaAI    BsaWI
    BsiEI    BsiWI    BspEI   BssHII   BstEII  BstZ17I    BtgZI   DraIII
     EagI  EcoICRI     FalI     FseI     FspI    FspAI     HpaI     KpnI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PvuI    RsrII
     SacI    SacII    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SwaI     XbaI     XhoI     XmaI
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