Restriction map for pF1KB9554
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        Sau3AI      ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AvaI          316  CCCGAG               C^[CT]CG[GA]G
BalI          427  TGGCCA               TGG^CCA
BanI          462  GGCACC               G^G[CT][GA]CC
BbsI          406  GTCTTC               GAAGAC(2/6)
BfaI          298  CTAG                 C^TAG
BpuAI         406  GTCTTC               GAAGAC(2/6)
BsaI          239  GAGACC               GGTCTC(1/5)
BsiEI          30  CGACCG               CG[GA][CT]^CG
BsmAI         239  GAGAC                GTCTC(1/5)
BsoBI         316  CCCGAG               C^[CT]CG[GA]G
BspMI         242  ACCTGC               ACCTGC(4/8)
BsrBI          33  CCGCTC               CCGCTC(-3/-3)
BstXI         137  CCATCTTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI          419  CCGTGG               C^C[GA][CT]GG
EaeI          427  TGGCCA               [CT]^GGCC[GA]
EarI          345  GAAGAG               CTCTTC(1/4)
FauI          459  GCGGG                CCCGC(4/6)
HinfI         497  GAGTC                G^A[ACGT]TC
Ksp632I       345  GAAGAG               CTCTTC(1/4)
MaeI          298  CTAG                 C^TAG
MaeIII         20  GTGAC                ^GT[ACGT]AC
MluNI         427  TGGCCA               TGG^CCA
MscI          427  TGGCCA               TGG^CCA
MslI          258  CATCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        131  CAGCTG               C[AC]G^C[GT]G
PflMI         284  CCAGGGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          497  GAGTC                GAGTC(4/5)
PvuII         131  CAGCTG               CAG^CTG
SmlI           91  CTCAAG               C^T[CT][GA]AG
Tsp45I         20  GTGAC                ^GT[GC]AC
Van91I        284  CCAGGGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          111  GTAC                 GT^AC
              414  GTAC                
AflIII        116  ACATGT               A^C[GA][CT]GT
              227  ACATGT              
AlwNI         247  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              439  CAGGAGCTG           
AspHI         310  GAGCTC               G[AT]GC[AT]^C
              363  GAGCTC              
BanII         310  GAGCTC               G[GA]GC[CT]^C
              363  GAGCTC              
BpmI          328  CTGGAG               CTGGAG(16/14)
              340  CTGGAG              
BseRI         268  GAGGAG               GAGGAG(10/8)
              271  GAGGAG              
BsiHKAI       310  GAGCTC               G[AT]GC[AT]^C
              363  GAGCTC              
BspLU11I      116  ACATGT               A^CATGT
              227  ACATGT              
Cfr13I        164  GGCCC                G^G[ACGT]CC
              321  GGGCC               
MboII         345  GAAGA                GAAGA(8/7)
              407  TCTTC               
NlaIII        117  CATG                 CATG^
              228  CATG                
NlaIV         205  GGCTCC               GG[ACGT]^[ACGT]CC
              462  GGCACC              
NspI          116  ACATGT               [GA]CATG^[CT]
              227  ACATGT              
RsaI          111  GTAC                 GT^AC
              414  GTAC                
SacI          310  GAGCTC               GAGCT^C
              363  GAGCTC              
Sau96I        164  GGCCC                G^G[ACGT]CC
              321  GGGCC               
SbfI          167  CCTGCAGG             CCTGCA^GG
              243  CCTGCAGG            
Sse8387I      167  CCTGCAGG             CCTGCA^GG
              243  CCTGCAGG            
XcmI          192  CCAGTGCGACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              469  CCACCTGGGGGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AluI        BbvI        BlpI        BmyI        
Bpu1102I    BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CelII       DdeI        EcoRII      Fnu4HI      FokI        
HaeIII      ItaI        MnlI        MvaI        MwoI        
PstI        ScrFI       SfaNI       SfcI        TseI        
TspRI       
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