Restriction map for pF1KB9553
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AfaI        
AflII       AflIII      AgeI        AhdI        AlwI        
AocI        ApaI        ApoI        AscI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmAI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco81I      EcoNI       
EcoO65I     EcoRI       EcoT22I     FbaI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp509I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         749  GACGTC               GACGT^C
AccI          696  GTCTAC               GT^[AC][GT]AC
AcyI          749  GACGTC               G[GA]^CG[CT]C
Alw44I        509  GTGCAC               G^TGCAC
Aor51HI       404  AGCGCT               AGC^GCT
ApaLI         509  GTGCAC               G^TGCAC
AseI          774  ATTAAT               AT^TAAT
AsnI          774  ATTAAT               AT^TAAT
AspI          633  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          205  CCCGAG               C^[CT]CG[GA]G
BanI          117  GGTGCC               G^G[CT][GA]CC
BbvCI         219  GCTGAGG              CCTCAGC(-5/-2)
BfaI          340  CTAG                 C^TAG
BglI           94  GCCGGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         506  TACGTG               [CT]AC^GT[GA]
BsaHI         749  GACGTC               G[GA]^CG[CT]C
BsgI          526  GTGCAG               GTGCAG(16/14)
BsmI          616  GCATTC               GAATGC(1/-1)
BsoBI         205  CCCGAG               C^[CT]CG[GA]G
BspMI         522  GCAGGT               ACCTGC(4/8)
BsrFI          94  GCCGGC               [GA]^CCGG[CT]
BssHII        172  GCGCGC               G^CGCGC
BstAPI        519  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I         94  GCCGGC               [GA]^CCGG[CT]
DpnII         265  GATC                 ^GATC
DsaI          562  CCATGG               C^C[GA][CT]GG
Eco47III      404  AGCGCT               AGC^GCT
EcoRV         603  GATATC               GAT^ATC
FseI           93  GGCCGGCC             GGCCGG^CC
Hin1I         749  GACGTC               G[GA]^CG[CT]C
HphI          541  TCACC                GGTGA(8/7)
MaeI          340  CTAG                 C^TAG
MaeIII        540  GTCAC                ^GT[ACGT]AC
MboI          265  GATC                 ^GATC
NaeI           94  GCCGGC               GCC^GGC
NcoI          562  CCATGG               C^CATGG
NdeII         265  GATC                 ^GATC
NgoMIV         94  GCCGGC               G^CCGGC
PflFI         633  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         774  ATTAAT               AT^TAAT
PstI          517  CTGCAG               CTGCA^G
SacI          684  GAGCTC               GAGCT^C
Sau3AI        265  GATC                 ^GATC
SfaNI         418  GATGC                GCATC(5/9)
SfcI          517  CTGCAG               C^T[GA][CT]AG
SfiI           93  GGCCGGCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp45I        540  GTCAC                ^GT[GC]AC
Tth111I       633  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII          18  CGCG                 CG^CG
              173  CGCG                
AspHI         509  GTGCAC               G[AT]GC[AT]^C
              684  GAGCTC              
BpmI          547  CTCCAG               CTGGAG(16/14)
              678  CTGGAG              
BsiEI          29  CGGCCG               CG[GA][CT]^CG
               92  CGGCCG              
BsiHKAI       509  GTGCAC               G[AT]GC[AT]^C
              684  GAGCTC              
BsmFI          37  GGGAC                GGGAC(10/14)
              747  GGGAC               
BsrBI         109  CCGCTC               CCGCTC(-3/-3)
              153  GAGCGG              
BstF5I        413  GGATG                GGATG(2/0)
              417  GGATG               
BstUI          18  CGCG                 CG^CG
              173  CGCG                
EagI           29  CGGCCG               C^GGCCG
               92  CGGCCG              
EclXI          29  CGGCCG               C^GGCCG
               92  CGGCCG              
Eco52I         29  CGGCCG               C^GGCCG
               92  CGGCCG              
EcoT14I       449  CCAAGG               C^C[AT][AT]GG
              562  CCATGG              
FauI           70  CCCGC                CCCGC(4/6)
              155  GCGGG               
FokI          413  GGATG                GGATG(9/13)
              417  GGATG               
MaeII         507  ACGT                 A^CGT
              750  ACGT                
MseI          397  TTAA                 T^TAA
              775  TTAA                
MvnI           18  CGCG                 CG^CG
              173  CGCG                
PpuMI          37  GGGACCC              [GA]G^G[AT]CC[CT]
              707  AGGACCC             
StyI          449  CCAAGG               C^C[AT][AT]GG
              562  CCATGG              
TaqI          267  TCGA                 T^CGA
              279  TCGA                
TfiI          567  GATTC                G^A[AT]TC
              591  GATTC               
Tru9I         397  TTAA                 T^TAA
              775  TTAA                
TspRI         470  CAGTG                CA[GC]TG[ACGT][ACGT]^
              742  CAGTG               
TthHB8I       267  TCGA                 T^CGA
              279  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        Ksp632I     
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PleI        Sau96I      
ScrFI       TseI        
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