(Circular) (Six-base) MAPSORT of: pF1KB9553.seq Check: 4368 from: 1 to: 3896 pF1KB9553 3896 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 621 621 Size: 3896 AatII G_ACGT'C Cuts at: 861 861 Size: 3896 AccI GT'mk_AC Cuts at: 805 934 805 Size: 129 3767 Acc65I G'GTAC_C Cuts at: 912 912 Size: 3896 AclI AA'CG_TT Cuts at: 890 3736 890 Size: 2846 1050 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 560 679 1721 2153 2900 3168 560 Size: 119 1042 432 747 268 1288 Fragments arranged by size: 1288 1042 747 432 268 119 AfeI AGC'GCT Cuts at: 514 514 Size: 3896 AflIII A'CryG_T Cuts at: 1248 2352 3329 1248 Size: 1104 977 1815 Fragments arranged by size: 1815 1104 977 AgeI A'CCGG_T Cuts at: 2284 2284 Size: 3896 AlwNI CAG_nnn'CTG Cuts at: 213 708 777 1201 2768 213 Size: 495 69 424 1567 1341 Fragments arranged by size: 1567 1341 495 424 69 ApaLI G'TGCA_C Cuts at: 617 2666 617 Size: 2049 1847 ApoI r'AATT_y Cuts at: 900 3249 3505 900 Size: 2349 256 1291 Fragments arranged by size: 2349 1291 256 AseI AT'TA_AT Cuts at: 20 883 20 Size: 863 3033 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3896 AvaI C'yCGr_G Cuts at: 313 916 313 Size: 603 3293 BamHI G'GATC_C Cuts at: 921 921 Size: 3896 BanI G'GyrC_C Cuts at: 225 912 1326 1574 1609 225 Size: 687 414 248 35 2512 Fragments arranged by size: 2512 687 414 248 35 BanII G_rGCy'C Cuts at: 198 313 796 910 1940 198 Size: 115 483 114 1030 2154 Fragments arranged by size: 2154 1030 483 115 114 BbeI G_GCGC'C Cuts at: 1330 1578 1330 Size: 248 3648 BbvCI CC'TCA_GC Cuts at: 328 328 Size: 3896 BciVI GTATCCnnnnn_n' Cuts at: 1794 2555 3896 1794 Size: 761 1341 1794 Fragments arranged by size: 1794 1341 761 BclI T'GATC_A Cuts at: 1416 1416 Size: 3896 BglI GCCn_nnn'nGGC Cuts at: 208 208 Size: 3896 BglII A'GATC_T Cuts at: 1411 3448 1411 Size: 2037 1859 BlpI GC'TnA_GC Cuts at: 1008 1008 Size: 3896 Bme1580I G_kGCm'C Cuts at: 621 1521 1614 2670 621 Size: 900 93 1056 1847 Fragments arranged by size: 1847 1056 900 93 BmrI ACTGGGnnnn_n' Cuts at: 636 738 814 1108 1189 1524 3668 636 Size: 102 76 294 81 335 2144 864 Fragments arranged by size: 2144 864 335 294 102 81 76 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 3896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 640 807 640 Size: 167 3729 Bpu10I CC'TnA_GC Cuts at: 307 328 307 Size: 21 3875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1072 1951 2443 2741 1072 Size: 879 492 298 2227 Fragments arranged by size: 2227 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3600 3600 Size: 3896 BsaAI yAC'GTr Cuts at: 616 1249 616 Size: 633 3263 BsaBI GATnn'nnATC Cuts at: 6 1410 3447 6 Size: 1404 2037 455 Fragments arranged by size: 2037 1404 455 BsaHI Gr'CG_yC Cuts at: 858 1327 1575 2277 3773 858 Size: 469 248 702 1496 981 Fragments arranged by size: 1496 981 702 469 248 BsaWI w'CCGG_w Cuts at: 1085 1606 2284 2558 2705 3116 1085 Size: 521 678 274 147 411 1865 Fragments arranged by size: 1865 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 331 345 559 804 331 Size: 14 214 245 3423 Fragments arranged by size: 3423 245 214 14 BseYI C'CCAG_C Cuts at: 1321 2079 2656 3152 1321 Size: 758 577 496 2065 Fragments arranged by size: 2065 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 655 655 Size: 3896 BsiEI CG_ry'CG Cuts at: 104 140 203 1438 1484 2692 104 Size: 36 63 1235 46 1208 1308 Fragments arranged by size: 1308 1235 1208 63 46 36 BsiHKAI G_wGCw'C Cuts at: 621 796 910 1688 1878 2670 621 Size: 175 114 778 190 792 1847 Fragments arranged by size: 1847 792 778 190 175 114 BsmI GAATG_Cn' Cuts at: 724 724 Size: 3896 Bsp1286I G_dGCh'C Cuts at: 198 313 621 796 910 1521 1614 1688 1878 Size: 115 308 175 114 611 93 74 190 Cuts at: 1878 1940 2670 198 Size: 62 730 1424 Fragments arranged by size: 1424 730 611 308 190 175 115 114 93 74 62 BspEI T'CCGG_A Cuts at: 3116 3116 Size: 3896 BspHI T'CATG_A Cuts at: 3072 3072 Size: 3896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 621 946 1160 1462 1843 621 Size: 325 214 302 381 2674 Fragments arranged by size: 2674 381 325 302 214 BsrBI CCG'CTC Cuts at: 50 219 263 2188 2242 3726 3891 50 Size: 169 44 1925 54 1484 165 55 Fragments arranged by size: 1925 1484 169 165 55 54 44 BsrDI GCAATG_nn' Cuts at: 1808 1808 Size: 3896 BsrFI r'CCGG_y Cuts at: 202 1894 2075 2284 202 Size: 1692 181 209 1814 Fragments arranged by size: 1814 1692 209 181 BssHII G'CGCG_C Cuts at: 280 280 Size: 3896 BssSI C'ACGA_G Cuts at: 2167 2525 2167 Size: 358 3538 BstAPI GCAn_nnn'nTGC Cuts at: 633 1273 633 Size: 640 3256 BstBI TT'CG_AA Cuts at: 2257 2257 Size: 3896 BstXI CCAn_nnnn'nTGG Cuts at: 114 114 Size: 3896 BstYI r'GATC_y Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102 Size: 920 467 23 335 246 1012 86 12 Cuts at: 3102 3448 1 Size: 346 449 Fragments arranged by size: 1012 920 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 670 107 Size: 563 3333 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1846 2027 2190 1846 Size: 181 163 3552 Fragments arranged by size: 3552 181 163 BtsI GCAGTG_nn' Cuts at: 1139 1139 Size: 3896 DraI TTT'AAA Cuts at: 895 895 Size: 3896 DrdI GACnn_nn'nnGTC Cuts at: 1602 2460 1602 Size: 858 3038 EaeI y'GGCC_r Cuts at: 137 200 204 581 1481 1655 2046 2073 3418 Size: 63 4 377 900 174 391 27 1345 Cuts at: 3418 137 Size: 615 Fragments arranged by size: 1345 900 615 391 377 174 63 27 4 EagI C'GGCC_G Cuts at: 137 200 1481 137 Size: 63 1281 2552 Fragments arranged by size: 2552 1281 63 EarI CTCTTCn'nnn_ Cuts at: 422 536 698 1919 2129 422 Size: 114 162 1221 210 2189 Fragments arranged by size: 2189 1221 210 162 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 174 565 2104 2414 2560 3706 174 Size: 391 1539 310 146 1146 364 Fragments arranged by size: 1539 1146 391 364 310 146 EcoICRI GAG'CTC Cuts at: 794 908 794 Size: 114 3782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 560 640 679 807 1721 2153 2900 3168 560 Size: 80 39 128 914 432 747 268 1288 Fragments arranged by size: 1288 914 747 432 268 128 80 39 EcoO109I rG'GnC_Cy Cuts at: 146 210 816 1035 146 Size: 64 606 219 3007 Fragments arranged by size: 3007 606 219 64 EcoRI G'AATT_C Cuts at: 900 900 Size: 3896 EcoRV GAT'ATC Cuts at: 713 713 Size: 3896 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 178 1915 178 Size: 1737 2159 FseI GG_CCGG'CC Cuts at: 206 206 Size: 3896 FspI TGC'GCA Cuts at: 1677 1677 Size: 3896 HaeII r_GCGC'y Cuts at: 297 328 516 1330 1578 2600 3579 297 Size: 31 188 814 248 1022 979 614 Fragments arranged by size: 1022 979 814 614 248 188 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 281 313 690 722 1738 1768 1770 1800 281 Size: 32 377 32 1016 30 2 30 2377 Fragments arranged by size: 2377 1016 377 32 32 30 30 2 HincII GTy'rAC Cuts at: 935 935 Size: 3896 KasI G'GCGC_C Cuts at: 1326 1574 1326 Size: 248 3648 KpnI G_GTAC'C Cuts at: 916 916 Size: 3896 MluI A'CGCG_T Cuts at: 3329 3329 Size: 3896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2567 2751 2567 Size: 184 3712 MscI TGG'CCA Cuts at: 1657 1657 Size: 3896 MslI CAynn'nnrTG Cuts at: 112 1885 2012 112 Size: 1773 127 1996 Fragments arranged by size: 1996 1773 127 MspA1I CmG'CkG Cuts at: 1007 1321 1681 2694 2939 1007 Size: 314 360 1013 245 1964 Fragments arranged by size: 1964 1013 360 314 245 NaeI GCC'GGC Cuts at: 204 2077 204 Size: 1873 2023 NarI GG'CG_CC Cuts at: 1327 1575 1327 Size: 248 3648 NcoI C'CATG_G Cuts at: 107 670 107 Size: 563 3333 NgoMIV G'CCGG_C Cuts at: 202 2075 202 Size: 1873 2023 NspI r_CATG'y Cuts at: 949 1449 2356 949 Size: 500 907 2489 Fragments arranged by size: 2489 907 500 PciI A'CATG_T Cuts at: 2352 2352 Size: 3896 PmeI GTTT'AAAC Cuts at: 895 895 Size: 3896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3035 3067 3035 Size: 32 3864 PpuMI rG'GwC_Cy Cuts at: 146 816 146 Size: 670 3226 PstI C_TGCA'G Cuts at: 629 943 1628 629 Size: 314 685 2897 Fragments arranged by size: 2897 685 314 PvuI CG_AT'CG Cuts at: 104 104 Size: 3896 PvuII CAG'CTG Cuts at: 1321 1681 1321 Size: 360 3536 RsrII CG'GwC_CG Cuts at: 2091 2091 Size: 3896 SacI G_AGCT'C Cuts at: 796 910 796 Size: 114 3782 SalI G'TCGA_C Cuts at: 933 933 Size: 3896 SanDI GG'GwC_CC Cuts at: 146 146 Size: 3896 SapI GCTCTTCn'nnn_ Cuts at: 1919 2129 1919 Size: 210 3686 SbfI CC_TGCA'GG Cuts at: 943 943 Size: 3896 ScaI AGT'ACT Cuts at: 3413 3413 Size: 3896 SfcI C'TryA_G Cuts at: 33 625 939 1624 2617 2808 33 Size: 592 314 685 993 191 1121 Fragments arranged by size: 1121 993 685 592 314 191 SfiI GGCCn_nnn'nGGCC Cuts at: 208 208 Size: 3896 SfoI GGC'GCC Cuts at: 1328 1576 1328 Size: 248 3648 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3896 SmaI CCC'GGG Cuts at: 918 918 Size: 3896 SmlI C'TyrA_G Cuts at: 1051 1966 2458 2720 1051 Size: 915 492 262 2227 Fragments arranged by size: 2227 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3896 SphI G_CATG'C Cuts at: 949 1449 949 Size: 500 3396 StyI C'CwwG_G Cuts at: 107 557 670 1030 1384 107 Size: 450 113 360 354 2619 Fragments arranged by size: 2619 450 360 354 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1479 1612 2071 2280 1479 Size: 133 459 209 3095 Fragments arranged by size: 3095 459 209 133 TatI w'GTAC_w Cuts at: 3411 3411 Size: 3896 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3413 3445 3413 Size: 32 3864 Tth111I GACn'n_nGTC Cuts at: 744 1693 744 Size: 949 2947 XbaI T'CTAG_A Cuts at: 927 927 Size: 3896 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 1391 114 Size: 1277 2619 XmaI C'CCGG_G Cuts at: 916 916 Size: 3896 ZraI GAC'GTC Cuts at: 859 859 Size: 3896 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI