################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BamHI BanI BbeI BcgI BciVI BfrI BglI BglII BlnI BpmI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI ClaI CpoI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AhdI 1437 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1373 GTGCAC G^TGCAC ApaLI 1373 GTGCAC G^TGCAC AspEI 1437 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 195 CCCGGG C^[CT]CG[GA]G BbrPI 970 CACGTG CAC^GTG BbsI 903 GAAGAC GAAGAC(2/6) BbvCI 927 CCTCAGC CCTCAGC(-5/-2) BclI 225 TGATCA T^GATCA BfaI 1474 CTAG C^TAG BpuAI 903 GAAGAC GAAGAC(2/6) BsaAI 970 CACGTG [CT]AC^GT[GA] BsaI 274 GAGACC GGTCTC(1/5) BsiYI 1388 CCAAGTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1388 CCAAGTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 195 CCCGGG C^[CT]CG[GA]G BspHI 270 TCATGA T^CATGA BspMI 1030 ACCTGC ACCTGC(4/8) BsrDI 452 CATTGC GCAATG(2/0) BssSI 506 CTCGTG CACGAG(-5/-1) BstBI 532 TTCGAA TT^CGAA DraI 919 TTTAAA TTT^AAA DraII 1157 AGGTCCT [GA]G^G[ACGT]CC[CT] Eam1105I 1437 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1157 AGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 225 TGATCA T^GATCA HaeIII 1357 GGCC GG^CC MaeI 1474 CTAG C^TAG NaeI 1394 GCCGGC GCC^GGC NgoMIV 1394 GCCGGC G^CCGGC NspV 532 TTCGAA TT^CGAA PmaCI 970 CACGTG CAC^GTG PmlI 970 CACGTG CAC^GTG PpuMI 1157 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 270 TCATGA T^CATGA SacI 85 GAGCTC GAGCT^C SfuI 532 TTCGAA TT^CGAA SmaI 195 CCCGGG CCC^GGG XmaCI 195 CCCGGG C^CCGGG XmaI 195 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 470 GTAC GT^AC 647 GTAC AflIII 108 ACATGT A^C[GA][CT]GT 1340 ACATGT AlwI 823 GATCC GGATC(4/5) 1537 GGATC AvaII 1158 GGTCC G^G[AT]CC 1179 GGACC BanII 85 GAGCTC G[GA]GC[CT]^C 1108 GAGCCC BcnI 195 CCCGG CC^[GC]GG 196 CCGGG BlpI 82 GCTGAGC GC^T[ACGT]AGC 124 GCTGAGC Bpu1102I 82 GCTGAGC GC^T[ACGT]AGC 124 GCTGAGC BsmAI 274 GAGAC GTCTC(1/5) 1310 GTCTC BsmFI 1301 GTCCC GGGAC(10/14) 1623 GTCCC BspLU11I 108 ACATGT A^CATGT 1340 ACATGT BsrFI 1046 ACCGGC [GA]^CCGG[CT] 1394 GCCGGC CelII 82 GCTGAGC GC^T[ACGT]AGC 124 GCTGAGC Cfr10I 1046 ACCGGC [GA]^CCGG[CT] 1394 GCCGGC MaeII 971 ACGT A^CGT 1270 ACGT MspA1I 930 CAGCTG C[AC]G^C[GT]G 1011 CAGCTG NciI 195 CCCGG CC^[GC]GG 196 CCGGG NspI 108 ACATGT [GA]CATG^[CT] 1340 ACATGT PvuII 930 CAGCTG CAG^CTG 1011 CAGCTG RsaI 470 GTAC GT^AC 647 GTAC SfcI 1072 CTACAG C^T[GA][CT]AG 1399 CTACAG XcmI 681 CCATGTTCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1182 CCATCATAAGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AcsI AluI ApoI Asp700I AspHI BbvI BmyI BsaJI BsiHKAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI