(Circular) (Six-base) MAPSORT of: pF1KB9545.seq Check: 9138 from: 1 to: 4742 pF1KB9545 4742 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1147 1147 Size: 4742 AccI GT'mk_AC Cuts at: 1780 1780 Size: 4742 Acc65I G'GTAC_C Cuts at: 1758 1758 Size: 4742 AclI AA'CG_TT Cuts at: 4582 4582 Size: 4742 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 506 689 704 1621 2567 2999 3352 4014 506 Size: 183 15 917 946 432 353 662 1234 Fragments arranged by size: 1234 946 917 662 432 353 183 15 AflIII A'CryG_T Cuts at: 216 1448 2094 3894 4175 216 Size: 1232 646 1800 281 783 Fragments arranged by size: 1800 1232 783 646 281 AhdI GACnn_n'nnGTC Cuts at: 1550 1550 Size: 4742 AleI CACnn'nnGTG Cuts at: 1488 1488 Size: 4742 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1682 1714 1682 Size: 32 4710 AlwNI CAG_nnn'CTG Cuts at: 2047 3485 2047 Size: 1438 3304 ApaLI G'TGCA_C Cuts at: 1481 3580 1481 Size: 2099 2643 ApoI r'AATT_y Cuts at: 261 699 1096 1398 1424 1718 1746 4095 4351 Size: 438 397 302 26 294 28 2349 256 Cuts at: 4351 261 Size: 652 Fragments arranged by size: 2349 652 438 397 302 294 256 28 26 AseI AT'TA_AT Cuts at: 20 20 Size: 4742 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4742 AvaI C'yCGr_G Cuts at: 303 1762 303 Size: 1459 3283 BamHI G'GATC_C Cuts at: 1767 1767 Size: 4742 BanI G'GyrC_C Cuts at: 1758 2172 2420 2455 1758 Size: 414 248 35 4045 Fragments arranged by size: 4045 414 248 35 BanII G_rGCy'C Cuts at: 197 1220 1756 2786 197 Size: 1023 536 1030 2153 Fragments arranged by size: 2153 1030 1023 536 BbeI G_GCGC'C Cuts at: 2176 2424 2176 Size: 248 4494 BbsI GAAGACnn'nnnn_ Cuts at: 1018 1018 Size: 4742 BbvCI CC'TCA_GC Cuts at: 1036 1036 Size: 4742 BciVI GTATCCnnnnn_n' Cuts at: 2640 3696 4742 2640 Size: 1056 1046 2640 Fragments arranged by size: 2640 1056 1046 BclI T'GATC_A Cuts at: 333 2262 333 Size: 1929 2813 BglII A'GATC_T Cuts at: 2257 4294 2257 Size: 2037 2705 BlpI GC'TnA_GC Cuts at: 191 233 1854 191 Size: 42 1621 3079 Fragments arranged by size: 3079 1621 42 Bme1580I G_kGCm'C Cuts at: 1485 2367 2460 3584 1485 Size: 882 93 1124 2643 Fragments arranged by size: 2643 1124 882 93 BmrI ACTGGGnnnn_n' Cuts at: 1479 1542 1954 2035 2370 4514 1479 Size: 63 412 81 335 2144 1707 Fragments arranged by size: 2144 1707 412 335 81 63 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3109 3109 Size: 4742 Bpu10I CC'TnA_GC Cuts at: 1036 1574 1036 Size: 538 4204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1918 2797 3511 3809 1918 Size: 879 714 298 2851 Fragments arranged by size: 2851 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 376 4446 376 Size: 4070 672 BsaAI yAC'GTr Cuts at: 1080 2095 1080 Size: 1015 3727 BsaBI GATnn'nnATC Cuts at: 6 2256 4293 6 Size: 2250 2037 455 Fragments arranged by size: 2250 2037 455 BsaHI Gr'CG_yC Cuts at: 2173 2421 3123 4619 2173 Size: 248 702 1496 2296 Fragments arranged by size: 2296 1496 702 248 BsaWI w'CCGG_w Cuts at: 1931 2452 3130 3541 3688 3962 1931 Size: 521 678 411 147 274 2711 Fragments arranged by size: 2711 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1682 1712 1682 Size: 30 4712 BseYI C'CCAG_C Cuts at: 1121 2167 2925 3590 3998 1121 Size: 1046 758 665 408 1865 Fragments arranged by size: 1865 1046 758 665 408 BsiEI CG_ry'CG Cuts at: 104 2284 2330 3560 104 Size: 2180 46 1230 1286 Fragments arranged by size: 2180 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 197 807 1485 1541 1678 1756 2534 2724 3584 Size: 610 678 56 137 78 778 190 860 Cuts at: 3584 197 Size: 1355 Fragments arranged by size: 1355 860 778 678 610 190 137 78 56 Bsp1286I G_dGCh'C Cuts at: 197 807 1220 1485 1541 1678 1756 2367 2460 Size: 610 413 265 56 137 78 611 93 Cuts at: 2460 2534 2724 2786 3584 197 Size: 74 190 62 798 1355 Fragments arranged by size: 1355 798 611 610 413 265 190 137 93 78 74 62 56 BspHI T'CATG_A Cuts at: 378 3174 378 Size: 2796 1946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1147 1792 2006 2308 2689 1147 Size: 645 214 302 381 3200 Fragments arranged by size: 3200 645 381 302 214 BsrBI CCG'CTC Cuts at: 50 3034 3088 4572 4737 50 Size: 2984 54 1484 165 55 Fragments arranged by size: 2984 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 559 2654 559 Size: 2095 2647 BsrFI r'CCGG_y Cuts at: 1154 1502 2740 2921 1154 Size: 348 1238 181 2975 Fragments arranged by size: 2975 1238 348 181 BssSI C'ACGA_G Cuts at: 614 3013 3721 614 Size: 2399 708 1635 Fragments arranged by size: 2399 1635 708 BstAPI GCAn_nnn'nTGC Cuts at: 2119 2119 Size: 4742 BstBI TT'CG_AA Cuts at: 641 3103 641 Size: 2462 2280 BstYI r'GATC_y Cuts at: 1 1767 2234 2257 2592 2838 3144 3156 3242 Size: 1766 467 23 335 246 306 12 86 Cuts at: 3242 4294 1 Size: 1052 449 Fragments arranged by size: 1766 1052 467 449 335 306 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4742 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2692 2873 3036 2692 Size: 181 163 4398 Fragments arranged by size: 4398 181 163 BtsI GCAGTG_nn' Cuts at: 407 1146 1205 1985 407 Size: 739 59 780 3164 Fragments arranged by size: 3164 780 739 59 DraI TTT'AAA Cuts at: 1029 1741 1029 Size: 712 4030 DrdI GACnn_nn'nnGTC Cuts at: 2448 3792 2448 Size: 1344 3398 EaeI y'GGCC_r Cuts at: 2327 2501 2892 2919 4264 2327 Size: 174 391 27 1345 2805 Fragments arranged by size: 2805 1345 391 174 27 EagI C'GGCC_G Cuts at: 2327 2327 Size: 4742 EarI CTCTTCn'nnn_ Cuts at: 122 525 1640 2765 2975 122 Size: 403 1115 1125 210 1889 Fragments arranged by size: 1889 1125 1115 403 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2950 3692 3838 4552 2950 Size: 742 146 714 3140 Fragments arranged by size: 3140 742 714 146 EcoICRI GAG'CTC Cuts at: 195 1754 195 Size: 1559 3183 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 506 689 704 1621 2567 2999 3352 4014 506 Size: 183 15 917 946 432 353 662 1234 Fragments arranged by size: 1234 946 917 662 432 353 183 15 EcoO109I rG'GnC_Cy Cuts at: 1266 1881 1266 Size: 615 4127 EcoRI G'AATT_C Cuts at: 1746 1746 Size: 4742 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2761 2761 Size: 4742 FspI TGC'GCA Cuts at: 2523 2523 Size: 4742 HaeII r_GCGC'y Cuts at: 2176 2424 3654 4425 2176 Size: 248 1230 771 2493 Fragments arranged by size: 2493 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 454 486 915 947 1331 1363 1536 1568 1645 Size: 32 429 32 384 32 173 32 77 Cuts at: 1645 1677 2584 2614 2616 2646 454 Size: 32 907 30 2 30 2550 Fragments arranged by size: 2550 907 429 384 173 77 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1781 1781 Size: 4742 KasI G'GCGC_C Cuts at: 2172 2420 2172 Size: 248 4494 KpnI G_GTAC'C Cuts at: 1762 1762 Size: 4742 MluI A'CGCG_T Cuts at: 4175 4175 Size: 4742 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3501 3685 3501 Size: 184 4558 MscI TGG'CCA Cuts at: 2503 2503 Size: 4742 MslI CAynn'nnrTG Cuts at: 221 1449 1488 1669 2731 2858 221 Size: 1228 39 181 1062 127 2105 Fragments arranged by size: 2105 1228 1062 181 127 39 MspA1I CmG'CkG Cuts at: 1040 1121 1853 2167 2527 3311 3556 1040 Size: 81 732 314 360 784 245 2226 Fragments arranged by size: 2226 784 732 360 314 245 81 NaeI GCC'GGC Cuts at: 1504 2923 1504 Size: 1419 3323 NarI GG'CG_CC Cuts at: 2173 2421 2173 Size: 248 4494 NcoI C'CATG_G Cuts at: 107 107 Size: 4742 NgoMIV G'CCGG_C Cuts at: 1502 2921 1502 Size: 1419 3323 NspI r_CATG'y Cuts at: 220 1452 1795 2295 3898 220 Size: 1232 343 500 1603 1064 Fragments arranged by size: 1603 1232 1064 500 343 PciI A'CATG_T Cuts at: 216 1448 3894 216 Size: 1232 2446 1064 Fragments arranged by size: 2446 1232 1064 PmeI GTTT'AAAC Cuts at: 1741 1741 Size: 4742 PmlI CAC'GTG Cuts at: 1080 1080 Size: 4742 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1682 1714 3188 3220 1682 Size: 32 1474 32 3204 Fragments arranged by size: 3204 1474 32 32 PpuMI rG'GwC_Cy Cuts at: 1266 1266 Size: 4742 PstI C_TGCA'G Cuts at: 1789 2474 1789 Size: 685 4057 PvuI CG_AT'CG Cuts at: 104 104 Size: 4742 PvuII CAG'CTG Cuts at: 1040 1121 2167 2527 1040 Size: 81 1046 360 3255 Fragments arranged by size: 3255 1046 360 81 RsrII CG'GwC_CG Cuts at: 2937 2937 Size: 4742 SacI G_AGCT'C Cuts at: 197 1756 197 Size: 1559 3183 SalI G'TCGA_C Cuts at: 1779 1779 Size: 4742 SapI GCTCTTCn'nnn_ Cuts at: 2765 2975 2765 Size: 210 4532 SbfI CC_TGCA'GG Cuts at: 1789 1789 Size: 4742 ScaI AGT'ACT Cuts at: 4259 4259 Size: 4742 SfcI C'TryA_G Cuts at: 33 1180 1507 1785 2470 3438 3629 33 Size: 1147 327 278 685 968 191 1146 Fragments arranged by size: 1147 1146 968 685 327 278 191 SfoI GGC'GCC Cuts at: 2174 2422 2174 Size: 248 4494 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4742 SmaI CCC'GGG Cuts at: 305 1764 305 Size: 1459 3283 SmlI C'TyrA_G Cuts at: 1897 2812 3526 3788 1897 Size: 915 714 262 2851 Fragments arranged by size: 2851 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4742 SphI G_CATG'C Cuts at: 1795 2295 1795 Size: 500 4242 StyI C'CwwG_G Cuts at: 107 1876 2230 107 Size: 1769 354 2619 Fragments arranged by size: 2619 1769 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1335 2325 2458 2917 3126 1335 Size: 990 133 459 209 2951 Fragments arranged by size: 2951 990 459 209 133 TatI w'GTAC_w Cuts at: 577 754 4257 577 Size: 177 3503 1062 Fragments arranged by size: 3503 1062 177 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1477 1509 4259 4291 1477 Size: 32 2750 32 1928 Fragments arranged by size: 2750 1928 32 32 Tth111I GACn'n_nGTC Cuts at: 2539 2539 Size: 4742 XbaI T'CTAG_A Cuts at: 1773 1773 Size: 4742 XcmI CCAnnnn_n'nnnnTGG Cuts at: 796 1297 2237 796 Size: 501 940 3301 Fragments arranged by size: 3301 940 501 XmaI C'CCGG_G Cuts at: 303 1762 303 Size: 1459 3283 XmnI GAAnn'nnTTC Cuts at: 492 702 1634 492 Size: 210 932 3600 Fragments arranged by size: 3600 932 210 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AgeI AlfI ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BpmI BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI