(Linear) (Six-base) MAPSORT of: KIBB9521.seq Check: 5911 from: 1 to: 1764 KIBB9521.orf With 182 enzymes: SgfI * March 3, 2008 11:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 319 1764 Size: 319 1445 Acc65I G'GTAC_C Cuts at: 0 700 720 1764 Size: 700 20 1044 Fragments arranged by size: 1044 700 20 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 531 1433 1764 Size: 531 902 331 Fragments arranged by size: 902 531 331 AfeI AGC'GCT Cuts at: 0 484 985 1764 Size: 484 501 779 Fragments arranged by size: 779 501 484 AleI CACnn'nnGTG Cuts at: 0 853 1764 Size: 853 911 AlwNI CAG_nnn'CTG Cuts at: 0 402 615 951 1764 Size: 402 213 336 813 Fragments arranged by size: 813 402 336 213 ApaI G_GGCC'C Cuts at: 0 624 1344 1732 1764 Size: 624 720 388 32 Fragments arranged by size: 720 624 388 32 AvaI C'yCGr_G Cuts at: 0 5 746 1268 1345 1764 Size: 5 741 522 77 419 Fragments arranged by size: 741 522 419 77 5 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 246 279 1764 Size: 246 33 1485 Fragments arranged by size: 1485 246 33 BamHI G'GATC_C Cuts at: 0 1720 1764 Size: 1720 44 BanI G'GyrC_C Cuts at: 0 700 720 855 900 1764 Size: 700 20 135 45 864 Fragments arranged by size: 864 700 135 45 20 BanII G_rGCy'C Cuts at: 0 74 624 639 1344 1732 1764 Size: 74 550 15 705 388 32 Fragments arranged by size: 705 550 388 74 32 15 BbeI G_GCGC'C Cuts at: 0 904 1764 Size: 904 860 BbsI GAAGACnn'nnnn_ Cuts at: 0 267 1764 Size: 267 1497 BbvCI CC'TCA_GC Cuts at: 0 106 379 1764 Size: 106 273 1385 Fragments arranged by size: 1385 273 106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 208 242 258 292 1555 1589 1764 Size: 208 34 16 34 1263 34 175 Fragments arranged by size: 1263 208 175 34 34 34 16 BglI GCCn_nnn'nGGC Cuts at: 0 1170 1764 Size: 1170 594 Bme1580I G_kGCm'C Cuts at: 0 172 197 624 860 1344 1568 1732 1764 Size: 172 25 427 236 484 224 164 32 Fragments arranged by size: 484 427 236 224 172 164 32 25 BmgBI CAC'GTC Cuts at: 0 999 1764 Size: 999 765 BmrI ACTGGGnnnn_n' Cuts at: 0 1600 1764 Size: 1600 164 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 48 1764 Size: 48 1716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 1764 Size: 163 1601 Bpu10I CC'TnA_GC Cuts at: 0 106 379 1764 Size: 106 273 1385 Fragments arranged by size: 1385 273 106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 871 1213 1764 Size: 871 342 551 Fragments arranged by size: 871 551 342 BsaHI Gr'CG_yC Cuts at: 0 901 1230 1764 Size: 901 329 534 Fragments arranged by size: 901 534 329 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 1054 1178 1408 1522 1562 1764 Size: 40 1014 124 230 114 40 202 Fragments arranged by size: 1014 230 202 124 114 40 40 BseYI C'CCAG_C Cuts at: 0 1326 1751 1764 Size: 1326 425 13 Fragments arranged by size: 1326 425 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 1273 1764 Size: 238 1035 491 Fragments arranged by size: 1035 491 238 BsiEI CG_ry'CG Cuts at: 0 323 1764 Size: 323 1441 BsiHKAI G_wGCw'C Cuts at: 0 937 1764 Size: 937 827 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1665 1764 Size: 1665 99 Bsp1286I G_dGCh'C Cuts at: 0 74 172 197 624 639 860 937 1344 Size: 74 98 25 427 15 221 77 407 Cuts at: 1344 1568 1732 1764 Size: 224 164 32 Fragments arranged by size: 427 407 224 221 164 98 77 74 32 25 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 319 792 1764 Size: 319 473 972 Fragments arranged by size: 972 473 319 BsrDI GCAATG_nn' Cuts at: 0 1024 1764 Size: 1024 740 BstAPI GCAn_nnn'nTGC Cuts at: 0 402 406 1764 Size: 402 4 1358 Fragments arranged by size: 1358 402 4 BstEII G'GTnAC_C Cuts at: 0 804 1764 Size: 804 960 BstXI CCAn_nnnn'nTGG Cuts at: 0 735 867 1764 Size: 735 132 897 Fragments arranged by size: 897 735 132 BstYI r'GATC_y Cuts at: 0 1720 1764 Size: 1720 44 BtgI C'CryG_G Cuts at: 0 212 495 938 1483 1764 Size: 212 283 443 545 281 Fragments arranged by size: 545 443 283 281 212 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 325 1764 Size: 325 1439 BtsI GCAGTG_nn' Cuts at: 0 400 448 1764 Size: 400 48 1316 Fragments arranged by size: 1316 400 48 ClaI AT'CG_AT Cuts at: 0 1526 1764 Size: 1526 238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 663 698 1764 Size: 663 35 1066 Fragments arranged by size: 1066 663 35 DraIII CAC_nnn'GTG Cuts at: 0 1010 1764 Size: 1010 754 DrdI GACnn_nn'nnGTC Cuts at: 0 1318 1764 Size: 1318 446 EaeI y'GGCC_r Cuts at: 0 86 116 305 593 706 1025 1427 1585 Size: 86 30 189 288 113 319 402 158 Cuts at: 1585 1764 Size: 179 Fragments arranged by size: 402 319 288 189 179 158 113 86 30 EarI CTCTTCn'nnn_ Cuts at: 0 1186 1400 1764 Size: 1186 214 364 Fragments arranged by size: 1186 364 214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 531 1433 1764 Size: 163 368 902 331 Fragments arranged by size: 902 368 331 163 EcoO109I rG'GnC_Cy Cuts at: 0 692 1140 1215 1320 1341 1351 1477 1489 Size: 692 448 75 105 21 10 126 12 Cuts at: 1489 1622 1728 1764 Size: 133 106 36 Fragments arranged by size: 692 448 133 126 106 105 75 36 21 12 10 FspI TGC'GCA Cuts at: 0 1003 1764 Size: 1003 761 FspAI rTGC'GCAy Cuts at: 0 1003 1764 Size: 1003 761 HaeII r_GCGC'y Cuts at: 0 82 304 486 824 904 987 1764 Size: 82 222 182 338 80 83 777 Fragments arranged by size: 777 338 222 182 83 82 80 HincII GTy'rAC Cuts at: 0 369 1764 Size: 369 1395 KasI G'GCGC_C Cuts at: 0 900 1764 Size: 900 864 KpnI G_GTAC'C Cuts at: 0 704 724 1764 Size: 704 20 1040 Fragments arranged by size: 1040 704 20 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 68 266 872 1364 1573 1623 1764 Size: 68 198 606 492 209 50 141 Fragments arranged by size: 606 492 209 198 141 68 50 MscI TGG'CCA Cuts at: 0 88 307 595 1027 1429 1764 Size: 88 219 288 432 402 335 Fragments arranged by size: 432 402 335 288 219 88 MslI CAynn'nnrTG Cuts at: 0 181 349 424 733 853 865 1764 Size: 181 168 75 309 120 12 899 Fragments arranged by size: 899 309 181 168 120 75 12 MspA1I CmG'CkG Cuts at: 0 190 222 497 1755 1764 Size: 190 32 275 1258 9 Fragments arranged by size: 1258 275 190 32 9 NarI GG'CG_CC Cuts at: 0 901 1764 Size: 901 863 NcoI C'CATG_G Cuts at: 0 938 1764 Size: 938 826 NspI r_CATG'y Cuts at: 0 348 409 1179 1764 Size: 348 61 770 585 Fragments arranged by size: 770 585 348 61 PflMI CCAn_nnn'nTGG Cuts at: 0 95 464 1446 1764 Size: 95 369 982 318 Fragments arranged by size: 982 369 318 95 PfoI T'CCnGG_A Cuts at: 0 1502 1764 Size: 1502 262 PpuMI rG'GwC_Cy Cuts at: 0 1140 1215 1320 1351 1477 1489 1622 1764 Size: 1140 75 105 31 126 12 133 142 Fragments arranged by size: 1140 142 133 126 105 75 31 12 PspOMI G'GGCC_C Cuts at: 0 620 1340 1728 1764 Size: 620 720 388 36 Fragments arranged by size: 720 620 388 36 PstI C_TGCA'G Cuts at: 0 803 1764 Size: 803 961 PvuII CAG'CTG Cuts at: 0 190 222 1755 1764 Size: 190 32 1533 9 Fragments arranged by size: 1533 190 32 9 SacII CC_GC'GG Cuts at: 0 498 1764 Size: 498 1266 SanDI GG'GwC_CC Cuts at: 0 1140 1477 1622 1764 Size: 1140 337 145 142 Fragments arranged by size: 1140 337 145 142 SapI GCTCTTCn'nnn_ Cuts at: 0 1186 1764 Size: 1186 578 SbfI CC_TGCA'GG Cuts at: 0 803 1764 Size: 803 961 SfcI C'TryA_G Cuts at: 0 799 1764 Size: 799 965 SfoI GGC'GCC Cuts at: 0 902 1764 Size: 902 862 SmlI C'TyrA_G Cuts at: 0 886 1192 1764 Size: 886 306 572 Fragments arranged by size: 886 572 306 SphI G_CATG'C Cuts at: 0 409 1764 Size: 409 1355 StuI AGG'CCT Cuts at: 0 31 1764 Size: 31 1733 StyI C'CwwG_G Cuts at: 0 695 938 1617 1764 Size: 695 243 679 147 Fragments arranged by size: 695 679 243 147 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 210 1764 Size: 210 1554 Enzymes that do cut: AarI Acc65I AcuI AfeI AleI AlwNI ApaI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EarI Eco57MI EcoO109I FspI FspAI HaeII HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SanDI SapI SbfI SfcI SfoI SmlI SphI StuI StyI XcmI Enzymes that do not cut: AatII AccI AclI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BciVI BclI BfrBI BglII BlpI BmtI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I DraI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI Hin4I HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI