(Circular) (Six-base) MAPSORT of: pF1KB9434.seq Check: 8117 from: 1 to: 3761 pF1KB9434 3761 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 667 667 Size: 3761 AccI GT'mk_AC Cuts at: 799 799 Size: 3761 Acc65I G'GTAC_C Cuts at: 777 777 Size: 3761 AclI AA'CG_TT Cuts at: 3601 3601 Size: 3761 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 142 259 535 1586 2018 2765 3033 142 Size: 117 276 1051 432 747 268 870 Fragments arranged by size: 1051 870 747 432 276 268 117 AfeI AGC'GCT Cuts at: 440 440 Size: 3761 AflIII A'CryG_T Cuts at: 294 517 1113 2217 3194 294 Size: 223 596 1104 977 861 Fragments arranged by size: 1104 977 861 596 223 AgeI A'CCGG_T Cuts at: 2149 2149 Size: 3761 AlwNI CAG_nnn'CTG Cuts at: 1066 2633 1066 Size: 1567 2194 ApaI G_GGCC'C Cuts at: 388 388 Size: 3761 ApaLI G'TGCA_C Cuts at: 2531 2531 Size: 3761 ApoI r'AATT_y Cuts at: 166 765 3114 3370 166 Size: 599 2349 256 557 Fragments arranged by size: 2349 599 557 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3761 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3761 AvaI C'yCGr_G Cuts at: 225 781 225 Size: 556 3205 BamHI G'GATC_C Cuts at: 786 786 Size: 3761 BanI G'GyrC_C Cuts at: 334 777 1191 1439 1474 334 Size: 443 414 248 35 2621 Fragments arranged by size: 2621 443 414 248 35 BanII G_rGCy'C Cuts at: 210 388 775 1805 210 Size: 178 387 1030 2166 Fragments arranged by size: 2166 1030 387 178 BbeI G_GCGC'C Cuts at: 1195 1443 1195 Size: 248 3513 BciVI GTATCCnnnnn_n' Cuts at: 1659 2420 3761 1659 Size: 761 1341 1659 Fragments arranged by size: 1659 1341 761 BclI T'GATC_A Cuts at: 1281 1281 Size: 3761 BglII A'GATC_T Cuts at: 554 1276 3313 554 Size: 722 2037 1002 Fragments arranged by size: 2037 1002 722 BlpI GC'TnA_GC Cuts at: 873 873 Size: 3761 Bme1580I G_kGCm'C Cuts at: 388 1386 1479 2535 388 Size: 998 93 1056 1614 Fragments arranged by size: 1614 1056 998 93 BmgBI CAC'GTC Cuts at: 600 600 Size: 3761 BmrI ACTGGGnnnn_n' Cuts at: 254 291 447 973 1054 1389 3533 254 Size: 37 156 526 81 335 2144 482 Fragments arranged by size: 2144 526 482 335 156 81 37 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 3761 Bpu10I CC'TnA_GC Cuts at: 209 209 Size: 3761 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 403 937 1816 2308 2606 403 Size: 534 879 492 298 1558 Fragments arranged by size: 1558 879 534 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3465 3465 Size: 3761 BsaAI yAC'GTr Cuts at: 1114 1114 Size: 3761 BsaBI GATnn'nnATC Cuts at: 6 1275 3312 6 Size: 1269 2037 455 Fragments arranged by size: 2037 1269 455 BsaHI Gr'CG_yC Cuts at: 1192 1440 2142 3638 1192 Size: 248 702 1496 1315 Fragments arranged by size: 1496 1315 702 248 BsaWI w'CCGG_w Cuts at: 950 1471 2149 2423 2570 2981 950 Size: 521 678 274 147 411 1730 Fragments arranged by size: 1730 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1186 1944 2521 3017 1186 Size: 758 577 496 1930 Fragments arranged by size: 1930 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 181 271 336 659 181 Size: 90 65 323 3283 Fragments arranged by size: 3283 323 90 65 BsiEI CG_ry'CG Cuts at: 104 621 1303 1349 2557 104 Size: 517 682 46 1208 1308 Fragments arranged by size: 1308 1208 682 517 46 BsiHKAI G_wGCw'C Cuts at: 775 1553 1743 2535 775 Size: 778 190 792 2001 Fragments arranged by size: 2001 792 778 190 Bsp1286I G_dGCh'C Cuts at: 210 388 775 1386 1479 1553 1743 1805 2535 Size: 178 387 611 93 74 190 62 730 Cuts at: 2535 210 Size: 1436 Fragments arranged by size: 1436 730 611 387 190 178 93 74 62 BspEI T'CCGG_A Cuts at: 2981 2981 Size: 3761 BspHI T'CATG_A Cuts at: 2937 2937 Size: 3761 BspMI ACCTGCnnnn'nnnn_ Cuts at: 117 667 811 1025 1327 1708 117 Size: 550 144 214 302 381 2170 Fragments arranged by size: 2170 550 381 302 214 144 BsrBI CCG'CTC Cuts at: 50 360 2053 2107 3591 3756 50 Size: 310 1693 54 1484 165 55 Fragments arranged by size: 1693 1484 310 165 55 54 BsrDI GCAATG_nn' Cuts at: 1673 1673 Size: 3761 BsrFI r'CCGG_y Cuts at: 1759 1940 2149 1759 Size: 181 209 3371 Fragments arranged by size: 3371 209 181 BssSI C'ACGA_G Cuts at: 2032 2390 2032 Size: 358 3403 BstAPI GCAn_nnn'nTGC Cuts at: 1138 1138 Size: 3761 BstBI TT'CG_AA Cuts at: 2122 2122 Size: 3761 BstYI r'GATC_y Cuts at: 1 554 566 786 1253 1276 1611 1857 2869 Size: 553 12 220 467 23 335 246 1012 Cuts at: 2869 2955 2967 3313 1 Size: 86 12 346 449 Fragments arranged by size: 1012 553 467 449 346 335 246 220 86 23 12 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1711 1892 2055 1711 Size: 181 163 3417 Fragments arranged by size: 3417 181 163 BtsI GCAGTG_nn' Cuts at: 1004 1004 Size: 3761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 503 538 503 Size: 35 3726 DraI TTT'AAA Cuts at: 760 760 Size: 3761 DrdI GACnn_nn'nnGTC Cuts at: 1467 2325 1467 Size: 858 2903 EaeI y'GGCC_r Cuts at: 1346 1520 1911 1938 3283 1346 Size: 174 391 27 1345 1824 Fragments arranged by size: 1824 1345 391 174 27 EagI C'GGCC_G Cuts at: 1346 1346 Size: 3761 EarI CTCTTCn'nnn_ Cuts at: 1784 1994 1784 Size: 210 3551 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1969 2279 2425 3571 1969 Size: 310 146 1146 2159 Fragments arranged by size: 2159 1146 310 146 EcoICRI GAG'CTC Cuts at: 773 773 Size: 3761 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 142 259 535 1586 2018 2765 3033 142 Size: 117 276 1051 432 747 268 870 Fragments arranged by size: 1051 870 747 432 276 268 117 EcoNI CCTnn'n_nnAGG Cuts at: 124 124 Size: 3761 EcoO109I rG'GnC_Cy Cuts at: 384 385 900 384 Size: 1 515 3245 Fragments arranged by size: 3245 515 1 EcoRI G'AATT_C Cuts at: 765 765 Size: 3761 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1780 1780 Size: 3761 FspI TGC'GCA Cuts at: 1542 1542 Size: 3761 HaeII r_GCGC'y Cuts at: 442 1195 1443 2465 3444 442 Size: 753 248 1022 979 759 Fragments arranged by size: 1022 979 759 753 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1603 1633 1635 1665 1603 Size: 30 2 30 3699 Fragments arranged by size: 3699 30 30 2 HincII GTy'rAC Cuts at: 654 800 654 Size: 146 3615 KasI G'GCGC_C Cuts at: 1191 1439 1191 Size: 248 3513 KpnI G_GTAC'C Cuts at: 781 781 Size: 3761 MluI A'CGCG_T Cuts at: 3194 3194 Size: 3761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 177 538 2432 2616 177 Size: 361 1894 184 1322 Fragments arranged by size: 1894 1322 361 184 MscI TGG'CCA Cuts at: 1522 1522 Size: 3761 MslI CAynn'nnrTG Cuts at: 631 691 1750 1877 631 Size: 60 1059 127 2515 Fragments arranged by size: 2515 1059 127 60 MspA1I CmG'CkG Cuts at: 872 1186 1546 2559 2804 872 Size: 314 360 1013 245 1829 Fragments arranged by size: 1829 1013 360 314 245 NaeI GCC'GGC Cuts at: 1942 1942 Size: 3761 NarI GG'CG_CC Cuts at: 1192 1440 1192 Size: 248 3513 NgoMIV G'CCGG_C Cuts at: 1940 1940 Size: 3761 NspI r_CATG'y Cuts at: 298 521 814 1314 2221 298 Size: 223 293 500 907 1838 Fragments arranged by size: 1838 907 500 293 223 PciI A'CATG_T Cuts at: 294 517 2217 294 Size: 223 1700 1838 Fragments arranged by size: 1838 1700 223 PmeI GTTT'AAAC Cuts at: 760 760 Size: 3761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2900 2932 2900 Size: 32 3729 PspOMI G'GGCC_C Cuts at: 384 384 Size: 3761 PstI C_TGCA'G Cuts at: 808 1493 808 Size: 685 3076 PvuI CG_AT'CG Cuts at: 104 104 Size: 3761 PvuII CAG'CTG Cuts at: 1186 1546 1186 Size: 360 3401 RsrII CG'GwC_CG Cuts at: 1956 1956 Size: 3761 SacI G_AGCT'C Cuts at: 775 775 Size: 3761 SalI G'TCGA_C Cuts at: 798 798 Size: 3761 SapI GCTCTTCn'nnn_ Cuts at: 1784 1994 1784 Size: 210 3551 SbfI CC_TGCA'GG Cuts at: 808 808 Size: 3761 ScaI AGT'ACT Cuts at: 3278 3278 Size: 3761 SfcI C'TryA_G Cuts at: 33 804 1489 2482 2673 33 Size: 771 685 993 191 1121 Fragments arranged by size: 1121 993 771 685 191 SfoI GGC'GCC Cuts at: 1193 1441 1193 Size: 248 3513 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3761 SmaI CCC'GGG Cuts at: 783 783 Size: 3761 SmlI C'TyrA_G Cuts at: 418 916 1831 2323 2585 418 Size: 498 915 492 262 1594 Fragments arranged by size: 1594 915 498 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3761 SphI G_CATG'C Cuts at: 814 1314 814 Size: 500 3261 SspI AAT'ATT Cuts at: 509 509 Size: 3761 StyI C'CwwG_G Cuts at: 895 1249 895 Size: 354 3407 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 273 514 625 1344 1477 1936 2145 273 Size: 241 111 719 133 459 209 1889 Fragments arranged by size: 1889 719 459 241 209 133 111 TatI w'GTAC_w Cuts at: 291 455 3276 291 Size: 164 2821 776 Fragments arranged by size: 2821 776 164 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3278 3310 3278 Size: 32 3729 Tth111I GACn'n_nGTC Cuts at: 622 1558 622 Size: 936 2825 XbaI T'CTAG_A Cuts at: 792 792 Size: 3761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1256 1256 Size: 3761 XmaI C'CCGG_G Cuts at: 781 781 Size: 3761 XmnI GAAnn'nnTTC Cuts at: 509 509 Size: 3761 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI