Restriction map for pF1KB9385
 (Linear) (Six-base) MAPSORT of: KIBB9385.seq  Check: 9154  from: 1  to: 972



KIBB9385.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

Acc65I G'GTAC_C

Cuts at:      0     821     972 
   Size:        821     151
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     834     941     972 
   Size:        834     107      31

  Fragments arranged by size: 

                 834     107      31
 
AflIII A'CryG_T

Cuts at:      0     107     972 
   Size:        107     865
 
AgeI A'CCGG_T

Cuts at:      0       4     972 
   Size:          4     968
 
AleI CACnn'nnGTG

Cuts at:      0     112     972 
   Size:        112     860
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     436     972 
   Size:         87     349     536

  Fragments arranged by size: 

                 536     349      87
 
AvaI C'yCGr_G

Cuts at:      0      37     972 
   Size:         37     935
 
BamHI G'GATC_C

Cuts at:      0     369     783     972 
   Size:        369     414     189

  Fragments arranged by size: 

                 414     369     189
 
BanI G'GyrC_C

Cuts at:      0     169     821     972 
   Size:        169     652     151

  Fragments arranged by size: 

                 652     169     151
 
BbeI G_GCGC'C

Cuts at:      0     173     972 
   Size:        173     799
 
BclI T'GATC_A

Cuts at:      0     258     972 
   Size:        258     714
 
BfrBI ATG'CAT

Cuts at:      0     243     972 
   Size:        243     729
 
BmgBI CAC'GTC

Cuts at:      0     110     972 
   Size:        110     862
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      75     700     972 
   Size:         75     625     272

  Fragments arranged by size: 

                 625     272      75
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     458     972 
   Size:        231     227     514

  Fragments arranged by size: 

                 514     231     227
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     972 
   Size:        136     836
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     434     972 
   Size:        434     538
 
BsaAI yAC'GTr

Cuts at:      0     507     972 
   Size:        507     465
 
BsaHI Gr'CG_yC

Cuts at:      0     170     972 
   Size:        170     802
 
BsaWI w'CCGG_w

Cuts at:      0       4     972 
   Size:          4     968
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      42      87     972 
   Size:         42      45     885

  Fragments arranged by size: 

                 885      45      42
 
BseYI C'CCAG_C

Cuts at:      0     391     972 
   Size:        391     581
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     529     972 
   Size:        529     443
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     124     972 
   Size:        124     848
 
BsrFI r'CCGG_y

Cuts at:      0       4     132     166     443     972 
   Size:          4     128      34     277     529

  Fragments arranged by size: 

                 529     277     128      34       4
 
BssSI C'ACGA_G

Cuts at:      0     828     972 
   Size:        828     144
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     844     972 
   Size:        844     128
 
BstYI r'GATC_y

Cuts at:      0     369     783     972 
   Size:        369     414     189

  Fragments arranged by size: 

                 414     369     189
 
Bsu36I CC'TnA_GG

Cuts at:      0     635     972 
   Size:        635     337
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     819     972 
   Size:        819     153
 
EaeI y'GGCC_r

Cuts at:      0     134     972 
   Size:        134     838
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     458     834     941     972 
   Size:        231     227     376     107      31

  Fragments arranged by size: 

                 376     231     227     107      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0     278     395     972 
   Size:        278     117     577

  Fragments arranged by size: 

                 577     278     117
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     286     675     972 
   Size:        286     389     297

  Fragments arranged by size: 

                 389     297     286
 
HaeII r_GCGC'y

Cuts at:      0     173     972 
   Size:        173     799
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     387     419     972 
   Size:         34      32     321      32     553

  Fragments arranged by size: 

                 553     321      34      32      32
 
KasI G'GCGC_C

Cuts at:      0     169     972 
   Size:        169     803
 
KpnI G_GTAC'C

Cuts at:      0     825     972 
   Size:        825     147
 
MfeI C'AATT_G

Cuts at:      0     232     972 
   Size:        232     740
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     972 
   Size:        223     749
 
MslI CAynn'nnrTG

Cuts at:      0     112     972 
   Size:        112     860
 
NaeI GCC'GGC

Cuts at:      0     134     972 
   Size:        134     838
 
NarI GG'CG_CC

Cuts at:      0     170     972 
   Size:        170     802
 
NgoMIV G'CCGG_C

Cuts at:      0     132     972 
   Size:        132     840
 
NsiI A_TGCA'T

Cuts at:      0     245     972 
   Size:        245     727
 
NspI r_CATG'y

Cuts at:      0     208     972 
   Size:        208     764
 
PfoI T'CCnGG_A

Cuts at:      0     176     972 
   Size:        176     796
 
PpuMI rG'GwC_Cy

Cuts at:      0     278     395     972 
   Size:        278     117     577

  Fragments arranged by size: 

                 577     278     117
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     487     519     972 
   Size:        487      32     453

  Fragments arranged by size: 

                 487     453      32
 
SanDI GG'GwC_CC

Cuts at:      0     278     972 
   Size:        278     694
 
SfoI GGC'GCC

Cuts at:      0     171     972 
   Size:        171     801
 
SgrAI Cr'CCGG_yG

Cuts at:      0     166     972 
   Size:        166     806
 
SmlI C'TyrA_G

Cuts at:      0     151     972 
   Size:        151     821
 
SphI G_CATG'C

Cuts at:      0     208     972 
   Size:        208     764
 
SspI AAT'ATT

Cuts at:      0     690     777     972 
   Size:        690      87     195

  Fragments arranged by size: 

                 690     195      87
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     900     932     972 
   Size:        900      32      40

  Fragments arranged by size: 

                 900      40      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     206     972 
   Size:        206     766
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AgeI     AleI    AlwNI     AvaI    BamHI
     BanI     BbeI     BclI    BfrBI    BmgBI     BmrI     BpmI    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsmBI
    BsrFI    BssSI   BstAPI    BstYI   Bsu36I    BtgZI     EaeI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I     KasI     KpnI     MfeI     MmeI
     MslI     NaeI     NarI   NgoMIV     NsiI     NspI     PfoI    PpuMI
     PsrI    SanDI     SfoI    SgrAI     SmlI     SphI     SspI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AhdI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BanII     BbsI    BbvCI     BcgI    BciVI     BglI    BglII
     BlpI Bme1580I     BmtI     BplI   Bpu10I    BsaBI    BsaXI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I     BtgI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     MluI     MscI   MspA1I     NcoI     NdeI
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI     SmaI    SnaBI     SpeI     SrfI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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